Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I2 R1 780 68.3 3343050 93.5% 3125751 102.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,153,047 A→G D370G (GAT→GGT)  fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,0470AG100.0% 238.8 / NA 65D370G (GAT→GGT) fabF3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/33);  total (32/33)

TGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152927‑1153171
                                                                                                                          |                                                                                                                            
tgtTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTg                                                                                                            <  1:854376/139‑1 (MQ=255)
    ggTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTaa                                                                                                                           <  2:1102758/122‑1 (MQ=255)
    ggTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTaa                                                                                                                           >  1:1102758/1‑122 (MQ=255)
    ggTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                             >  2:1226053/1‑134 (MQ=255)
    ggTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGtt                                                                                                             <  1:1226053/134‑1 (MQ=255)
     gTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATg                                                                                                                   >  2:1485827/1‑127 (MQ=255)
     gTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATg                                                                                                                   <  1:1485827/127‑1 (MQ=255)
          cTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAt                                                                                                  >  2:1401825/1‑139 (MQ=255)
          cTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAt                                                                                                  >  1:635181/1‑139 (MQ=255)
              aCGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGa                                                                                                                  <  2:798388/119‑1 (MQ=255)
              aCGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGa                                                                                                                  >  1:798388/1‑119 (MQ=255)
                gAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                            <  2:1282407/139‑1 (MQ=255)
                          aCCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  2:1405699/139‑1 (MQ=255)
                            cGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAgcg                                                                                >  2:1240939/1‑139 (MQ=255)
                              gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      <  2:2271/115‑1 (MQ=255)
                              gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                      >  1:2271/1‑115 (MQ=255)
                               tCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                             <  1:1401825/139‑1 (MQ=255)
                               tCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGt                                                                             <  1:1240939/139‑1 (MQ=255)
                                           tGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 >  2:721768/1‑139 (MQ=255)
                                             cggcggGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGAt                                                                                                        <  2:933925/98‑1 (MQ=255)
                                             cggcggGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGAt                                                                                                        >  1:933925/1‑98 (MQ=255)
                                             cggcggGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                               >  2:118967/1‑139 (MQ=255)
                                                cggGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatgga                                                            >  1:138254/1‑139 (MQ=255)
                                                   gTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATa                                                         <  1:721768/139‑1 (MQ=255)
                                                   gTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATa                                                         <  2:892916/139‑1 (MQ=255)
                                                                   ctaCTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAcc                                                                                                                         >  1:1108566/1‑59 (MQ=255)
                                                                   ctaCTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAAcc                                                                                                                         <  2:1108566/59‑1 (MQ=255)
                                                                   ctaCTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTcc                                         >  1:1558536/1‑139 (MQ=255)
                                                                      cTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttc                                      >  2:39232/1‑139 (MQ=255)
                                                                         cATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggc                                   >  2:1447243/1‑139 (MQ=255)
                                                                           tCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                           <  1:1050635/81‑1 (MQ=255)
                                                                           tCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTAc                                                                                           >  2:1050635/1‑81 (MQ=255)
                                                                            ccTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttc                                >  2:496076/1‑139 (MQ=255)
                                                                               g‑‑gCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGt                             <  2:138254/139‑1 (MQ=255)
                                                                               g‑‑gCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGt                             <  1:1447243/139‑1 (MQ=255)
                                                                                  gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTg                            <  1:496076/139‑1 (MQ=255)
                                                                                   cgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAg                                                                                  <  1:1036893/84‑1 (MQ=255)
                                                                                   cgcTGCGCGATCAGGCTGTTCCGCCAACAATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATATCGTACCGCACGAAg                                                                                  >  2:1036893/1‑84 (MQ=255)
                                                                                       gcgcGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGt                                                                        <  2:1621226/90‑1 (MQ=255)
                                                                                       gcgcGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGt                                                                        >  1:1621226/1‑90 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 >  2:1566734/1‑96 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                 <  1:1566734/96‑1 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  2:1353037/1‑139 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  1:1001780/1‑139 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  1:1636023/1‑139 (MQ=255)
                                                                                        cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                      >  2:1457771/1‑139 (MQ=255)
                                                                                         gcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                     <  2:1558536/139‑1 (MQ=255)
                                                                                           gATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                   <  2:1001780/139‑1 (MQ=255)
                                                                                            aTCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTa                                                                      <  1:1201796/87‑1 (MQ=255)
                                                                                            aTCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTa                                                                      >  2:1201796/1‑87 (MQ=255)
                                                                                                 gCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                               <  2:1365131/89‑1 (MQ=255)
                                                                                                 gCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                               >  1:1365131/1‑89 (MQ=255)
                                                                                                   tGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTg           <  1:1457771/139‑1 (MQ=255)
                                                                                                     ttCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGAt         >  2:1573991/1‑139 (MQ=255)
                                                                                                       ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                     >  2:443796/1‑125 (MQ=255)
                                                                                                       ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                     <  1:443796/125‑1 (MQ=255)
                                                                                                       ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCt       >  1:744053/1‑139 (MQ=255)
                                                                                                           cAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                   >  2:1344511/1‑75 (MQ=255)
                                                                                                           cAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCg                                                                   <  1:1344511/75‑1 (MQ=255)
                                                                                                           cAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaa   <  1:1353037/139‑1 (MQ=255)
                                                                                                            aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa  <  2:1636023/139‑1 (MQ=255)
                                                                                                            aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa  <  1:1573991/139‑1 (MQ=255)
                                                                                                            aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa  <  2:744053/139‑1 (MQ=255)
                                                                                                                    aCCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTTTAACTCctgcggcttc                                >  1:5997/1‑101 (MQ=255)
                                                                                                                    aCCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCcttcggcttc                                <  2:5997/101‑1 (MQ=255)
                                                                                                                          |                                                                                                                            
TGTTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTG‑‑GCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAA  >  NC_000913/1152927‑1153171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A