Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,153,047 A→G D370G (GAT→GGT)  fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,153,0470AG98.2% 187.3 / ‑4.7 54D370G (GAT→GGT) fabF3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (28/25);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

TTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTA  >  NC_000913/1152929‑1153179
                                                                                                                      |                                                                                                                                    
ttGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCg                                                                                                                  <  2:1003708/139‑1 (MQ=255)
        cTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAt                                                                                                          >  2:192691/1‑139 (MQ=255)
                     aTGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGc                                                                                                                   <  2:958630/117‑1 (MQ=255)
                     aTGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGc                                                                                                                   >  1:958630/1‑117 (MQ=255)
                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAtt                                                                                                         <  2:895142/120‑1 (MQ=255)
                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGAtt                                                                                                         >  1:895142/1‑120 (MQ=255)
                            gTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGcgcg                                                                                      >  2:617977/1‑139 (MQ=255)
                                     tAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                             <  1:1202600/139‑1 (MQ=255)
                                     tAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCACACGAAGCGCGTCAGGTTAg                                                                             <  2:519031/139‑1 (MQ=255)
                                      aGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGa                                                                                            >  1:237628/1‑123 (MQ=255)
                                      aGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGa                                                                                            <  2:237628/123‑1 (MQ=255)
                                          gcggcgGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaa                                                                        <  2:258897/139‑1 (MQ=255)
                                          gcggcgGGTGCAGTAGAAACTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaa                                                                        >  1:258897/1‑139 (MQ=255)
                                           cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                       <  1:617977/139‑1 (MQ=255)
                                           cggcggGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCAACAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                       >  2:325385/1‑139 (MQ=255)
                                                ggTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaatggaat                                                                  >  2:1130635/1‑139 (MQ=255)
                                                     aGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACAct                                                             <  1:325385/139‑1 (MQ=255)
                                                           atctatctACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTgtgt                                                       <  1:192691/139‑1 (MQ=255)
                                                            tctatctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTa                                                      >  2:1004530/1‑139 (MQ=255)
                                                               atctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCa                                                                                               <  1:154636/95‑1 (MQ=255)
                                                               atctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCa                                                                                               >  2:154636/1‑95 (MQ=255)
                                                                 ctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAAc                                                    <  1:772256/136‑1 (MQ=255)
                                                                 ctaCTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTcc                                                 >  2:548203/1‑139 (MQ=255)
                                                                 ctaCTCCATCCTGGCGCCGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTTCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAAc                                                    >  2:772256/1‑136 (MQ=255)
                                                                       cATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                       >  2:1042044/1‑111 (MQ=255)
                                                                       cATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCggaat                                                                       <  1:1042044/111‑1 (MQ=255)
                                                                         tCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                              >  2:314880/1‑70 (MQ=255)
                                                                         tCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                              <  1:314880/70‑1 (MQ=255)
                                                                          ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                              >  1:12114/1‑69 (MQ=255)
                                                                          ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCt                                                                                                              <  2:12114/69‑1 (MQ=255)
                                                                          ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATa                                                                 >  1:1093363/1‑114 (MQ=255)
                                                                          ccTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATa                                                                 <  2:1093363/114‑1 (MQ=255)
                                                                             ggCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGt                                     <  1:1130635/139‑1 (MQ=255)
                                                                              gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                             >  1:755800/1‑98 (MQ=255)
                                                                              gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                             <  2:755800/98‑1 (MQ=255)
                                                                              gcgcTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTg                                    >  1:1338569/1‑139 (MQ=255)
                                                                                 cTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCa                                 >  2:402724/1‑139 (MQ=255)
                                                                                    tgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                              >  2:840996/2‑139 (MQ=255)
                                                                                    cgcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTa                              >  1:396699/1‑139 (MQ=255)
                                                                                     gcgATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                             <  2:1338569/139‑1 (MQ=255)
                                                                                       gATCAGGCTGTTCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATg                           <  1:548203/139‑1 (MQ=255)
                                                                                                 ttCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                         >  2:331422/1‑83 (MQ=255)
                                                                                                 ttCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCgga                                                                         <  1:331422/83‑1 (MQ=255)
                                                                                                 ttCCGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGAt                 >  1:1174211/1‑139 (MQ=255)
                                                                                                   ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                                    >  1:1224846/1‑54 (MQ=255)
                                                                                                   ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTa                                                                                                    <  2:1224846/54‑1 (MQ=255)
                                                                                                   ccGCCAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTACTTTGATCt               >  2:795448/1‑139 (MQ=255)
                                                                                                      ccAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                             >  1:964635/1‑122 (MQ=255)
                                                                                                      ccAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTaa                             <  2:964635/122‑1 (MQ=255)
                                                                                                       cAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                             <  1:243388/73‑1 (MQ=255)
                                                                                                       cAACCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAg                                                                             >  2:243388/1‑73 (MQ=255)
                                                                                                        aaCCATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaa          <  1:530597/139‑1 (MQ=255)
                                                                                                          ccATCAACCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTaaaaa        <  2:1174211/139‑1 (MQ=255)
                                                                                                                aCCTGGGTAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTa  >  1:216346/1‑139 (MQ=255)
                                                                                                                      |                                                                                                                                    
TTGGTAAGCTCCACGAAATCTATGACCGGTCACCTGTTAGGTGCGGCGGGTGCAGTAGAATCTATCTACTCCATCCTGGCGCTGCGCGATCAGGCTGTTCCGCCAACCATCAACCTGGATAACCCGGATGAAGGTTGCGATCTGGATTTCGTACCGCACGAAGCGCGTCAGGTTAGCGGAATGGAATACACTCTGTGTAACTCCTTCGGCTTCGGTGGCACTAATGGTTCTTTGATCTTTAAAAAGATCTA  >  NC_000913/1152929‑1153179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A