Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,605,180 |
A→G |
S44G (AGC→GGC) |
lsrB → |
autoinducer 2‑binding protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,605,180 | 0 | A | G | 100.0%
| 95.7
/ NA
| 28 | S44G (AGC→GGC) | lsrB | autoinducer 2‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/15); total (13/15) |
TTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAG > NC_000913/1605058‑1605300
|
ttCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcgc > 1:167105/1‑139 (MQ=255)
tCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAAGGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGTCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa < 2:392470/139‑1 (MQ=255)
cGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaac > 1:602736/1‑139 (MQ=255)
cTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGGGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCAcaacaa < 1:208499/139‑1 (MQ=255)
cgcTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAt > 1:1159050/1‑139 (MQ=255)
gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGTGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg > 1:769478/1‑127 (MQ=255)
gCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtg < 2:769478/127‑1 (MQ=255)
tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcg > 1:621514/1‑75 (MQ=255)
tgCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGgcg < 2:621514/75‑1 (MQ=255)
cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGa < 2:637253/124‑1 (MQ=255)
cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGa > 1:637253/1‑124 (MQ=255)
cAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGttt > 2:1244364/1‑139 (MQ=255)
gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTc < 2:1159050/139‑1 (MQ=255)
gCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTc < 2:167105/139‑1 (MQ=255)
gggCGTATTACATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCCACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAgg < 2:763388/137‑1 (MQ=255)
cGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTAc < 2:602736/139‑1 (MQ=255)
cATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacga < 1:837127/97‑1 (MQ=255)
cATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTacga > 2:837127/1‑97 (MQ=255)
ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgt < 2:204606/85‑1 (MQ=255)
ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGAtgt > 1:204606/1‑85 (MQ=255)
ttATTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATtaa > 1:1059346/1‑139 (MQ=255)
aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa < 2:246907/54‑1 (MQ=255)
aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCacaa > 1:246907/1‑54 (MQ=255)
aTTCCCAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGCTGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATtaata > 1:744894/1‑139 (MQ=255)
cAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAg > 1:750981/1‑105 (MQ=255)
cAAACTGGTTGGCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCCACAGAATCCAg < 2:750981/105‑1 (MQ=255)
ttGGCGTGGAATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGtcaatca < 2:869442/139‑1 (MQ=255)
ggCGTGGGATTTTTTACCGGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAg < 2:1059346/139‑1 (MQ=255)
|
TTCATCGCTTTAAGAAAATCGCCTTACTTAGCGCTCTTGGCATTGCCGCAATCTCTATGAATGTGCAGGCCGCAGAGCGTATTGCATTTATTCCCAAACTGGTTGGCGTGGGATTTTTTACCAGCGGTGGCAACGGCGCACAACAAGCGGGTAAAGAGCTGGGCGTTGATGTGACCTACGACGGGCCGACAGAACCCAGTGTTTCTGGTCAGGTACAGTTGATTAATAACTTCGTCAATCAAG > NC_000913/1605058‑1605300
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
