Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,626,825	C→T	V186I (GTT→ATT)	dcp ←	dipeptidyl carboxypeptidase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,626,825	0	C	T	100.0%	84.3 / NA	27	V186I (GTT→ATT)	dcp	dipeptidyl carboxypeptidase II
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/12); total (15/12)

AGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACA  >  NC_000913/1626716‑1626952

agcagcGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTa                                                                                                    >  1:994517/1‑139 (MQ=255)

     cGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGc                                                                                               >  2:143879/1‑139 (MQ=255)

     cGGAATCAGCAATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGt                                                                                                     >  2:833437/1‑133 (MQ=255)

     cGGAATCAGCAATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGt                                                                                                     <  1:833437/133‑1 (MQ=255)

                 tttGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAActgg                                                                                   <  2:685617/139‑1 (MQ=255)

                              ccTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                      <  1:953876/139‑1 (MQ=255)

                                 ttCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                   <  1:338524/139‑1 (MQ=255)

                                  tctcGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGc                                                                  >  2:1199633/1‑139 (MQ=255)

                                                         aGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGCTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAAtt                                           >  2:766690/1‑139 (MQ=255)

                                                                  tctTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTAt                                  <  1:1199633/139‑1 (MQ=255)

                                                                     tGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAg                               >  1:446836/1‑139 (MQ=255)

                                                                            tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTa                                                           >  1:1187552/1‑104 (MQ=255)

                                                                            tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTa                                                           <  2:1279863/104‑1 (MQ=255)

                                                                            tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTa                                                           <  2:1187552/104‑1 (MQ=255)

                                                                            tcaTTCCTGCCAGCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTa                                                           >  1:1279863/1‑104 (MQ=255)

                                                                                        gCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                   >  2:1097054/1‑84 (MQ=255)

                                                                                        gCTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAg                                                                   <  1:1097054/84‑1 (MQ=255)

                                                                                         cTGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCg           >  1:577604/1‑139 (MQ=255)

                                                                                          tGCGCGATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCgg          >  2:967482/1‑139 (MQ=255)

                                                                                             gcgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCa                                                       <  2:1240015/91‑1 (MQ=255)

                                                                                             gcgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCa                                                       >  1:1240015/1‑91 (MQ=255)

                                                                                              cgATATCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAg      >  2:291994/1‑139 (MQ=255)

                                                                                                  atCGTTCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGCTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACa  <  1:766690/139‑1 (MQ=255)

                                                                                                      ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                      <  1:65970/67‑1 (MQ=255)

                                                                                                      ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCg                                                                      >  2:65970/1‑67 (MQ=255)

                                                                                                      ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTGTGGCTCCGGc         >  1:761484/1‑128 (MQ=255)

                                                                                                      ttCACAATCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCATGCGCAAGTTTGGCTCCGGc         <  2:761484/128‑1 (MQ=255)

AGCAGCGGAATCAGCCATTTGTTATCCAGACCTTTCTCGCGAGCCGCCTCTGCCGCCAGCGCAATCTCTTGCTCACTCATTCCTGCCAGCTGCGCGATATCGTTCACAACCAGACCGCCGGATTTATTTGCTGCCAGTAATCGCTGGTTAAACTGGCTGGTCAGGGTCGCAGCTTCTGTATTCAGTACTTTTAATTTTGCTTTATCAGCTTGCGCAAGTTTGGCTCCGGCAAGGACA  >  NC_000913/1626716‑1626952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A