Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,696,452 (A)5→4 intergenic (‑381/+10) uidA ← / ← uidR beta‑D‑glucuronidase/transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,696,4480A.100.0% 216.7 / NA 51intergenic (‑377/+14)uidA/uidRbeta‑D‑glucuronidase/transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (24/27);  total (24/27)

GGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NC_000913/1696317‑1696582
                                                                                                                                   |                                                                                                                                      
ggAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                                 <  1:851184/139‑5 (MQ=255)
  aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAagagaga                                                                                                                              <  2:570666/139‑1 (MQ=255)
     gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGaa                                                                                                                           >  2:580337/1‑139 (MQ=255)
     gATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGaa                                                                                                                           >  2:474447/1‑139 (MQ=255)
      aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAAt                                                                                                                          >  2:799809/1‑139 (MQ=255)
      aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAAt                                                                                                                          >  2:216218/1‑139 (MQ=255)
           ctctCATGACATGTGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCTGCCCGCCG‑AAAAGAGAGAGAATCAGGa                                                                                                                     <  2:770475/139‑1 (MQ=255)
            tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGAt                                                                                                                    <  1:474447/139‑1 (MQ=255)
               cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCg                                                                                                                 >  1:524393/1‑139 (MQ=255)
                 tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGt                                                                                                               <  2:201614/139‑1 (MQ=255)
                 tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGt                                                                                                               <  1:471814/139‑1 (MQ=255)
                          gCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATAcc                                                                                                      >  1:742089/1‑139 (MQ=255)
                               aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                                 >  2:1139931/1‑139 (MQ=255)
                               aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                                 >  2:941007/1‑139 (MQ=255)
                                   aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                             >  1:35947/1‑139 (MQ=255)
                                    atatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcatc                                                                                            <  1:941007/139‑1 (MQ=255)
                                          tGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  1:216218/139‑1 (MQ=255)
                                             ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                                 <  2:872474/94‑5 (MQ=255)
                                             ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaagaga                                                                                                                                 >  1:872474/1‑90 (MQ=255)
                                                              cGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                                 >  2:1128220/1‑124 (MQ=255)
                                                              cGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                                 <  1:1128220/124‑1 (MQ=255)
                                                                ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaag                                                                                                                                    <  1:332870/72‑2 (MQ=255)
                                                                ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGaaaag                                                                                                                                    >  2:332870/1‑71 (MQ=255)
                                                                ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGc                                                                                                                  <  1:911456/89‑1 (MQ=255)
                                                                ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGc                                                                                                                  >  2:911456/1‑89 (MQ=255)
                                                                ggAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCtt                                                                >  1:876624/1‑139 (MQ=255)
                                                                      aTAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCAcgcg                                                          <  2:876624/139‑1 (MQ=255)
                                                                              tGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCt                                                  <  1:1139931/139‑1 (MQ=255)
                                                                               gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCtt                                                 >  1:937957/1‑139 (MQ=255)
                                                                                cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  1:799809/139‑1 (MQ=255)
                                                                                cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                                <  2:742089/139‑1 (MQ=255)
                                                                                          ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  1:580337/139‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      <  2:1206226/86‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGt                                                                                      >  1:1206226/1‑86 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:859170/139‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:937957/139‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  1:630653/139‑1 (MQ=255)
                                                                                               atatGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCCGc                                 >  2:859170/1‑139 (MQ=255)
                                                                                                 atGAAACGCGGGTACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                              <  2:232909/108‑1 (MQ=255)
                                                                                                 atGAAACGCGGGTACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                              >  1:232909/1‑108 (MQ=255)
                                                                                                 atGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:1088291/137‑1 (MQ=255)
                                                                                                 atGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:1088291/1‑137 (MQ=255)
                                                                                                    aaaCGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  2:35947/139‑1 (MQ=255)
                                                                                                    aaaCGCGGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                            <  2:524393/139‑1 (MQ=255)
                                                                                                        gcgGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 >  1:1222932/1‑130 (MQ=255)
                                                                                                        gcgGGCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                                 <  2:1222932/130‑1 (MQ=255)
                                                                                                           ggCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGAt                                                                                                                    <  2:329299/44‑1 (MQ=38)
                                                                                                           ggCACAGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGAt                                                                                                                    >  1:329299/1‑44 (MQ=255)
                                                                                                                aGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGAt                                                                                                                    <  2:286709/39‑1 (MQ=38)
                                                                                                                aGTTATCACCAGCCCGCCG‑AAAAGAGAGAGAATCAGGAt                                                                                                                    >  1:286709/1‑39 (MQ=38)
                                                                                                                            cccGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      >  1:432367/1‑105 (MQ=255)
                                                                                                                            cccGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      >  1:113360/1‑105 (MQ=255)
                                                                                                                            cccGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:113360/105‑1 (MQ=255)
                                                                                                                            cccGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                      <  2:432367/105‑1 (MQ=255)
                                                                                                                            cccGCCG‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa    >  2:616115/1‑139 (MQ=255)
                                                                                                                              cgccg‑AAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTaaa  >  2:1238277/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTAAA  >  NC_000913/1696317‑1696582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A