Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,862,634 |
A→G |
intergenic (‑205/‑137) |
msrB ← / → gapA |
methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,862,634 | 0 | A | G | 100.0%
| 51.0
/ NA
| 16 | intergenic (‑205/‑137) | msrB/gapA | methionine sulfoxide reductase B/glyceraldehyde‑3‑phosphate dehydrogenase A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/10); total (6/10) |
TTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGAA > NC_000913/1862505‑1862767
|
ttttGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAACGG‑AGAGTGGGGCGagtcag < 1:862966/139‑1 (MQ=255)
tcGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca < 2:1021817/139‑1 (MQ=255)
tCGTGATGTAAATCACATTTTTTTAGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGGGGTCTCAAGCCTAAATGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGAt < 2:326827/139‑1 (MQ=255)
tAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt < 1:162307/85‑1 (MQ=255)
tAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCtt > 2:162307/1‑85 (MQ=255)
aTTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTaa < 2:1218098/139‑1 (MQ=255)
ggCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAg > 2:806003/1‑139 (MQ=255)
cATGTGGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTa < 2:779319/139‑1 (MQ=255)
tgGTCTCAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCTATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCa < 1:806003/139‑1 (MQ=255)
tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGt > 1:727939/1‑39 (MQ=255)
tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGt < 2:727939/39‑1 (MQ=255)
tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca < 1:187248/51‑1 (MQ=255)
tctcAAGCCCAAAGGAAGAGTGGGGCGAGTCAGTCGCGTAATGCTTAGGca > 2:187248/1‑51 (MQ=255)
gagTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACt > 1:244936/1‑121 (MQ=255)
gagTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACt < 2:244936/121‑1 (MQ=255)
gagTGGGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGaa > 1:1285824/1‑139 (MQ=255)
|
TTTTGTCCTAAACTTGATCTCGACGAAATGGCTGCACCTAAATCGTGATGAAAATCACATTTTTATCGTAATTGCCCTTTAAAATTCGGGGCGCCGACCCCATGTGGTCTCAAGCCCAAAGGAAGAGTGAGGCGAGTCAGTCGCGTAATGCTTAGGCACAGGATTGATTTGTCGCAATGATTGACACGATTCCGCTTGACGCTGCGTAAGGTTTTTGTAATTTTACAGGCAACCTTTTATTCACTAACAAATAGCTGGTGGAA > NC_000913/1862505‑1862767
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
