Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
750 |
51.0 |
2595496 |
93.6% |
2429384 |
106.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,931,976 |
(G)5→6 |
coding (1096/1179 nt) |
purT → |
phosphoribosylglycinamide formyltransferase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,931,971 | 1 | . | G | 100.0%
| 106.6
/ NA
| 30 | L364R (CTG→CGG) | purT | phosphoribosylglycinamide formyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (13/17); total (13/17) |
CTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCC > NC_000913/1931839‑1932088
|
cTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTGTATTTGGTAAGCCGGAAATTGGTGGAAGCCGTCGTCT‑gggggg > 2:944117/1‑138 (MQ=255)
gCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCa < 2:1133130/139‑1 (MQ=255)
ttCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACt < 2:32681/139‑1 (MQ=255)
ttCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACt < 1:865724/139‑1 (MQ=255)
ttCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑ggggggtggtact > 1:238919/1‑131 (MQ=255)
tCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTg > 1:1287407/1‑139 (MQ=255)
ccAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACg > 1:292171/1‑139 (MQ=255)
ccAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg < 1:270615/106‑2 (MQ=255)
ccAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg > 2:270615/1‑105 (MQ=255)
tCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg > 2:834856/1‑101 (MQ=255)
tCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg < 1:834856/102‑2 (MQ=255)
aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAAcgcgcg > 1:90346/1‑139 (MQ=255)
aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg < 2:667043/92‑2 (MQ=255)
aCGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑gggggg > 1:667043/1‑91 (MQ=255)
tGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCa < 1:340430/139‑1 (MQ=255)
gATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAAAGTGTTGTTGACGCCATTGAACGCGCGAAGCa < 2:768186/138‑1 (MQ=255)
gATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATCGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCTCGAAGCa > 1:768186/1‑138 (MQ=255)
gtgCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCa < 1:387782/85‑1 (MQ=255)
gtgCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCa > 2:387782/1‑85 (MQ=255)
gtgCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGaa > 2:307429/1‑121 (MQ=255)
gtgCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGaa < 1:307429/121‑1 (MQ=255)
ccGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCTGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTaa < 2:184376/139‑1 (MQ=255)
cGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTaaa > 2:881139/1‑139 (MQ=255)
tAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTAc < 1:649346/80‑1 (MQ=255)
tAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTAc > 2:649346/1‑80 (MQ=255)
ggCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGTACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTa < 2:238919/139‑1 (MQ=255)
tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCTGTCGTCTGGGGGGTGGCa < 2:553587/53‑1 (MQ=255)
tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCTGTCGTCTGGGGGGTGGCa > 1:553587/1‑53 (MQ=255)
tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCt < 2:361321/42‑1 (MQ=255)
tCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCt > 1:361321/1‑42 (MQ=255)
ttATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaa < 2:90346/139‑1 (MQ=255)
tATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGTCaaaaaa > 1:80217/1‑139 (MQ=255)
ggTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaaa > 1:1295732/1‑135 (MQ=255)
ggTAAGCCGGAAATTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaaa < 2:1295732/135‑1 (MQ=255)
aaTTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGTCAAAAAAACGCTTCAAAAATGcc < 2:80217/139‑1 (MQ=255)
aaTTGATGGCAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGcc < 1:881139/139‑1 (MQ=255)
cAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGa < 1:37506/80‑1 (MQ=255)
cAGCCGTCGTCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGa > 2:37506/1‑80 (MQ=255)
cgtcgtCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACa > 1:1211200/1‑78 (MQ=255)
cgtcgtCTGGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACa < 2:1211200/78‑1 (MQ=255)
|
CTGCAGCTTCTGCCGTTATTCTGCCACAACTGACCAGTCAGAATGTCACGTTTGATAATGTGCAGAATGCCGTAGGCGCAGATTTGCAGATTCGTTTATTTGGTAAGCCGGAAATTGATGGCAGCCGTCGTCT‑GGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCC > NC_000913/1931839‑1932088
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
