Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
757 |
76.1 |
3598070 |
93.4% |
3360597 |
108.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,934,813 |
A→G |
intergenic (‑209/+26) |
edd ← / ← zwf |
6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,934,813 | 0 | A | G | 100.0%
| 158.0
/ NA
| 44 | intergenic (‑209/+26) | edd/zwf | 6‑phosphogluconate dehydratase/glucose‑6‑phosphate 1‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/23); total (21/23) |
GTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATC > NC_000913/1934684‑1934944
|
gTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATaa > 2:1701307/1‑139 (MQ=255)
ccGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGa < 1:1353138/139‑1 (MQ=255)
cGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGAt < 2:622117/139‑1 (MQ=255)
cTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcg < 1:534422/139‑1 (MQ=255)
aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc < 2:401504/130‑1 (MQ=255)
aaaaaCGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc > 1:401504/1‑130 (MQ=255)
ttCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTc > 2:711012/1‑139 (MQ=255)
gtgtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAgg < 1:711012/139‑1 (MQ=255)
gtAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGaa > 2:609919/1‑139 (MQ=255)
aaaaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCAt < 1:1701307/139‑1 (MQ=255)
aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTaa < 2:1309221/86‑1 (MQ=255)
aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTaa > 1:1309221/1‑86 (MQ=255)
aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc < 2:1246600/103‑1 (MQ=255)
aaaGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAgcgc > 1:1246600/1‑103 (MQ=255)
ccTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACggg < 1:1493446/139‑1 (MQ=255)
tGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCg < 1:1021613/139‑1 (MQ=255)
aCGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAAc > 1:300306/1‑65 (MQ=255)
aCGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAAc < 2:300306/65‑1 (MQ=255)
ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt > 2:924606/1‑93 (MQ=255)
ggCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt < 1:924606/93‑1 (MQ=255)
gCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACg > 1:514385/1‑139 (MQ=255)
aGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc < 1:609919/139‑1 (MQ=255)
tgtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAg > 2:568489/1‑139 (MQ=255)
gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCa > 1:295613/1‑52 (MQ=255)
gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCa < 2:295613/52‑1 (MQ=255)
gtAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAgg > 1:942772/1‑139 (MQ=255)
tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc < 1:1367667/135‑1 (MQ=255)
tAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTcccc > 2:1367667/1‑135 (MQ=255)
aaaaTTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc < 2:942772/139‑1 (MQ=255)
ttGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGc < 2:514385/139‑1 (MQ=255)
gcgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCg > 1:1449938/1‑139 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt > 2:796898/1‑60 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt < 1:796898/60‑1 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACggg > 2:1621182/1‑109 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACggg < 1:1621182/109‑1 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc > 1:1777685/1‑120 (MQ=255)
cgcAAGATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTc < 2:1777685/120‑1 (MQ=255)
gATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGatat < 1:1552867/45‑1 (MQ=255)
gATCATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGatat > 2:1552867/1‑45 (MQ=255)
cATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACg > 2:63280/1‑65 (MQ=255)
cATGTTACCGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACg < 1:63280/65‑1 (MQ=255)
cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt > 1:624556/1‑44 (MQ=255)
cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACt < 2:624556/44‑1 (MQ=255)
cGGTAAAGTAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATc > 2:1305940/1‑139 (MQ=255)
|
GTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAAAGCCTCGTGGGTGAATCCGCACGAGGCCTGAAAGTGTAAAAATTGTTCTACAATCTGCGCAAGATCATGTTACCGGTAAAATAACCATAAAGGATAAGCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATC > NC_000913/1934684‑1934944
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
