Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,814,218	C→T	172 (TAG→TAA)	luxS ←	S‑ribosylhomocysteine lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,814,218	0	C	T	100.0%	134.1 / NA	42	172 (TAG→TAA)	luxS	S‑ribosylhomocysteine lyase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/21); total (21/21)

GTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCAC  >  NC_000913/2814083‑2814351

gTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                    >  2:1273015/1‑139 (MQ=255)

     gctgGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGAtgtg                                                                                                                               >  2:776593/1‑139 (MQ=255)

         gggCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGt                                                                                                                           <  1:296590/139‑1 (MQ=255)

                              ccGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                      <  2:995814/139‑1 (MQ=255)

                                 ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAAc                                                                                                                  >  2:776887/1‑124 (MQ=255)

                                 ccATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAAc                                                                                                                  <  1:776887/124‑1 (MQ=255)

                                         cgcAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGttc                                                                                           <  2:23609/139‑1 (MQ=255)

                                              gTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGt                                                                                      >  1:1056743/1‑139 (MQ=255)

                                                  cggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                    <  2:88357/89‑1 (MQ=255)

                                                  cggcggAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAg                                                                                                                                    >  1:88357/1‑89 (MQ=255)

                                                        aGGCGGTGCGCACTAGGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt                                                                            >  2:703966/1‑139 (MQ=255)

                                                                 gcACTAGGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTc                                                                   <  1:703966/139‑1 (MQ=255)

                                                                  cACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                                  <  2:251732/139‑1 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTTTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                    <  2:798444/138‑1 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTTTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                    >  1:798444/1‑138 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                                                                                                 <  2:1067745/93‑1 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGc                                                                                                 >  1:1067745/1‑93 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                    <  2:1138633/138‑1 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCt                                                    >  1:1138633/1‑138 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                   >  1:972129/1‑139 (MQ=255)

                                                                                 aGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                   >  2:1078795/1‑139 (MQ=255)

                                                                                 aGCCAGTACATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTa                                                   >  2:848899/1‑139 (MQ=255)

                                                                                   ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                      <  2:1027273/86‑1 (MQ=255)

                                                                                   ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGc                                                                                                      >  1:1027273/1‑86 (MQ=255)

                                                                                   ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                                  <  2:998901/122‑1 (MQ=255)

                                                                                   ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCa                                                                  >  1:998901/1‑122 (MQ=255)

                                                                                   ccAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTAcg                                                 >  2:384743/1‑139 (MQ=255)

                                                                                      gTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACgcgc                                              >  2:197423/1‑139 (MQ=255)

                                                                                       ttCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCa                                             >  2:1261599/1‑139 (MQ=255)

                                                                                               aaCTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTg                                     <  1:186141/139‑1 (MQ=255)

                                                                                                     cTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt                                                                            <  1:11020/94‑1 (MQ=255)

                                                                                                     cTTTTTTCAATTAATTGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGAt                                                                            >  2:11020/1‑94 (MQ=255)

                                                                                                              attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGa                      >  1:1274524/1‑139 (MQ=255)

                                                                                                              attaattGTGAAGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGa                      >  1:384305/1‑139 (MQ=255)

                                                                                                                         aGATAGTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGt           <  2:1274524/139‑1 (MQ=255)

                                                                                                                              gTTTACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTg      <  1:1261599/139‑1 (MQ=255)

                                                                                                                               tttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                                                           >  2:342562/1‑85 (MQ=255)

                                                                                                                               tttACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCa                                                           <  1:342562/85‑1 (MQ=255)

                                                                                                                                 tACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCca   <  1:124783/139‑1 (MQ=255)

                                                                                                                                 tACTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCca   <  1:1078795/139‑1 (MQ=255)

                                                                                                                                  aCTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTgcg                                   >  2:244302/1‑106 (MQ=255)

                                                                                                                                  aCTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTgcg                                   <  1:244302/106‑1 (MQ=255)

                                                                                                                                  aCTGATTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCcac  <  2:972129/139‑1 (MQ=255)

GTCCTGCTGGGGCTGGTGTGGTTTGCTAAACCGCCATTTGGCGCAGGTGGCGGCGGAGGCGGTGCGCACTAAGTACAACTAAGCCAGTTCATTTGAACTGGCTTTTTTCAATTAATTGTGAAGATAGTTTACTGACTAGATGTGCAGTTCCTGCAACTTCTCTTTCGGCAGTGCCAGTTCTTCGTTGCTGTTGATGCGTACGTCACGTTCCAGAATGCTACGCGCAATATCCTGCGCTTCCTGCAACGAGTGCATCTGGTAAGTGCCAC  >  NC_000913/2814083‑2814351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A