Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	750	51.0	2595496	93.6%	2429384	106.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,094,234	T→C	T283A (ACA→GCA)	yggR ←	putative PilT family AAA+ ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,094,234	0	T	C	100.0%	163.3 / NA	45	T283A (ACA→GCA)	yggR	putative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21)

GTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGG  >  NC_000913/3094099‑3094340

gTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGtt                                                                                                         >  2:183853/1‑139 (MQ=255)

       aCGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                  <  1:183853/139‑1 (MQ=255)

          cccTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAAt                                                                                               <  2:407697/139‑1 (MQ=255)

                         gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                    >  2:1141434/1‑119 (MQ=255)

                         gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                    <  1:1141434/119‑1 (MQ=255)

                               aTAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                <  2:57539/133‑1 (MQ=255)

                               aTAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                >  1:57539/1‑133 (MQ=255)

                                          gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                               <  2:636323/139‑1 (MQ=255)

                                          gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                               <  1:1120865/139‑1 (MQ=255)

                                             aCGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCAc                                                            >  1:107417/1‑139 (MQ=255)

                                              cGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttc                                                                        >  2:1181691/1‑126 (MQ=255)

                                              cGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttc                                                                        <  1:1181691/126‑1 (MQ=255)

                                                ttATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTc                                                         >  2:742488/1‑139 (MQ=255)

                                                     atcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCt                                                    >  2:1172529/1‑139 (MQ=255)

                                                       ccccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTg                                                                     >  1:389622/1‑120 (MQ=255)

                                                       ccccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTg                                                                     <  2:389622/120‑1 (MQ=255)

                                                       ccccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCttt                                                  <  1:833026/139‑1 (MQ=255)

                                                       ccccACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCttt                                                  >  2:1210757/1‑139 (MQ=255)

                                                       cccaACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAAGTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCttt                                                  >  2:833026/1‑139 (MQ=255)

                                                           aCCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTtgtg                                              >  2:707055/1‑139 (MQ=255)

                                                                ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                          >  1:693346/1‑106 (MQ=255)

                                                                ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                          <  2:693346/106‑1 (MQ=255)

                                                                 tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                              >  2:264543/1‑101 (MQ=255)

                                                                 tgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                              <  1:264543/101‑1 (MQ=255)

                                                                  gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                       >  1:919879/1‑139 (MQ=255)

                                                                  gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                       <  1:1210757/139‑1 (MQ=255)

                                                                  gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                       <  2:919879/139‑1 (MQ=255)

                                                                   cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACt                                      >  2:293817/1‑139 (MQ=255)

                                                                     cGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGc                                    <  2:107417/139‑1 (MQ=255)

                                                                                      ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                   >  2:1030509/1‑139 (MQ=255)

                                                                                              gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                              >  1:1008825/1‑72 (MQ=255)

                                                                                              gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                              <  2:1008825/72‑1 (MQ=255)

                                                                                              gtggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTa           >  1:18378/1‑139 (MQ=255)

                                                                                               tggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                       >  2:473528/1‑78 (MQ=255)

                                                                                               tggGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                       <  1:473528/78‑1 (MQ=255)

                                                                                                gggTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACg         >  1:371692/1‑139 (MQ=255)

                                                                                                   ttttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                <  1:830600/65‑1 (MQ=255)

                                                                                                   ttttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                >  2:830600/1‑65 (MQ=255)

                                                                                                       cccTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgggg  <  1:707055/139‑1 (MQ=255)

                                                                                                            cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg    >  2:1058242/1‑132 (MQ=255)

                                                                                                            cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg    <  1:1058242/132‑1 (MQ=255)

                                                                                                                           ccACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTa                               >  2:263033/1‑90 (MQ=255)

                                                                                                                           ccACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTa                               <  1:263033/90‑1 (MQ=255)

                                                                                                                             aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCa       >  2:599900/1‑112 (MQ=255)

                                                                                                                             aCCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCa       <  1:599900/112‑1 (MQ=255)

GTCACAAACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGG  >  NC_000913/3094099‑3094340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A