Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I2 R1	762	35.5	1545288	95.5%	1475750	117.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,094,234	T→C	T283A (ACA→GCA)	yggR ←	putative PilT family AAA+ ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,094,234	0	T	C	100.0%	151.5 / NA	43	T283A (ACA→GCA)	yggR	putative PilT family AAA+ ATPase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/23); total (20/23)

AACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTC  >  NC_000913/3094105‑3094348

aaCGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAg                                                                                                           <  2:748618/139‑1 (MQ=255)

       ttCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCa                                                                                                    >  1:665943/1‑139 (MQ=255)

                   gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                            <  1:247087/119‑1 (MQ=255)

                   gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCa                                                                                                            >  2:247087/1‑119 (MQ=255)

                   gTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                        <  2:665943/139‑1 (MQ=255)

                    tGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgc                                                                                       >  1:157167/1‑139 (MQ=255)

                    tGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgc                                                                                       >  1:441843/1‑139 (MQ=255)

                     gCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcgcg                                                                                      <  1:769165/139‑1 (MQ=255)

                         aTAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcct                                                                                  >  1:727617/1‑139 (MQ=255)

                          tAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGGTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtcctt                                                                                 <  2:441843/139‑1 (MQ=255)

                           aaCTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttc                                                                                <  2:519284/139‑1 (MQ=255)

                              tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                               >  2:645242/1‑121 (MQ=255)

                              tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                               <  1:645242/121‑1 (MQ=255)

                              tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTg                                                                             >  1:49156/1‑139 (MQ=255)

                              tctGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTg                                                                             >  1:723258/1‑139 (MQ=255)

                                 gctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                               <  1:547691/118‑1 (MQ=255)

                                 gctgAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAg                                                                                               >  2:547691/1‑118 (MQ=255)

                                    gAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATc                                                                       <  2:98639/139‑1 (MQ=255)

                                            atcatcCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCa                                                               <  2:727617/139‑1 (MQ=255)

                                                       ctgctgCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGAc                                                    >  1:522465/1‑139 (MQ=255)

                                                          ctgcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGc                                                 >  1:700791/1‑139 (MQ=255)

                                                            gcCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCAc                                               <  1:368292/139‑1 (MQ=255)

                                                             cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                          >  1:15606/1‑79 (MQ=255)

                                                             cccGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGc                                                                                                          <  2:15606/79‑1 (MQ=255)

                                                               cGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGc                                            <  2:49156/139‑1 (MQ=255)

                                                                  tttGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTa                                                                                                                <  1:485214/68‑1 (MQ=255)

                                                                  tttGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTa                                                                                                                >  2:485214/1‑68 (MQ=255)

                                                                       aTAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCACGGGTGCGTTAATCAGCAATTCAAGTAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAAc                                    >  1:472507/1‑139 (MQ=255)

                                                                           cATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGt                                                                                     <  1:743973/86‑1 (MQ=255)

                                                                           cATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGt                                                                                     >  2:743973/1‑86 (MQ=255)

                                                                             tGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTg                              <  1:580277/139‑1 (MQ=255)

                                                                                ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                        >  1:592294/1‑78 (MQ=255)

                                                                                ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCAcg                                                                                        <  2:592294/78‑1 (MQ=255)

                                                                                ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                               >  2:399321/1‑87 (MQ=255)

                                                                                ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGtccttcc                                                                               <  1:399321/87‑1 (MQ=255)

                                                                                ggTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCa                           <  1:686543/139‑1 (MQ=255)

                                                                                  tAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTTCCTGCCAGt                         <  2:119913/139‑1 (MQ=255)

                                                                                     cTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGTCCGTAAACTACCTGCCAGTTGa                      >  1:194237/1‑139 (MQ=255)

                                                                                            gTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCa               >  1:633924/1‑139 (MQ=255)

                                                                                               ttCCCTTCGCGAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACgg            <  2:633924/139‑1 (MQ=255)

                                                                                                      cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTGCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCATTTGATTACGCACGGGGt         <  2:442014/135‑1 (MQ=255)

                                                                                                      cgcgAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGt         >  1:442014/1‑135 (MQ=255)

                                                                                                         gAATCAAATTCCCCACCGCGGGTGCGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTc  <  2:366246/139‑1 (MQ=255)

AACGCCCTTCCCCCACCCGGTGCTGATAACTCTGCTGAAACGTTATCATCCCCACCTGCTGCCCGGTTTGAATAACATGCGGTAACTGGTGGGTTTTCCCTTCGCGAATCAAATTCCCCACCGCGGGTGTGTTAATCAGCAATTCAAATAGCGCCACGCGTCCTTCCTGTTTATCCACTTCCAGCTTTTGTGACAGCACTGCCCGTAAACTACCTGCCAGTTGATTACGCACGGGGTCTTTTTC  >  NC_000913/3094105‑3094348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A