Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I1 R1 750 51.0 2595496 93.6% 2429384 106.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,400,093 G→A A332V (GCG→GTG)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,400,0930GA100.0% 174.9 / NA 54A332V (GCG→GTG) mreBcell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/25);  total (29/25)

GGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTC  >  NC_000913/3399971‑3400226
                                                                                                                          |                                                                                                                                     
ggCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       <  1:479044/139‑1 (MQ=255)
                     tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  >  2:197064/1‑139 (MQ=255)
                     tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  >  1:1160596/1‑139 (MQ=255)
                     tctcGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTACGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTc                                                                                                  <  2:47153/139‑1 (MQ=255)
                        cGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTc                                                                                               >  2:1107192/1‑139 (MQ=255)
                         gTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       >  2:93795/1‑114 (MQ=255)
                         gTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcg                                                                                                                       <  1:93795/114‑1 (MQ=255)
                            tCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGca                                                                                           >  1:39872/1‑139 (MQ=255)
                              aGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         <  2:670063/139‑1 (MQ=255)
                                aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgcc                                                                                                                                <  1:1218379/98‑1 (MQ=255)
                                aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTgcc                                                                                                                                >  2:1218379/1‑98 (MQ=255)
                                aCCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaacaa                                                                                       >  2:832012/1‑139 (MQ=255)
                                    ggcaggGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGAc                                                                                   >  2:660192/1‑139 (MQ=255)
                                       aggGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTgg                                                                                <  2:1160596/139‑1 (MQ=255)
                                          gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTTGCCGCCGTGCATGTCGATCATGTCTAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCGACAACGACTGGAAt                                                                             >  1:793765/1‑139 (MQ=255)
                                          gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     >  1:226797/1‑115 (MQ=255)
                                          gTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     <  2:226797/115‑1 (MQ=255)
                                                   cacTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttc                                                                    >  2:1233450/1‑139 (MQ=255)
                                                      ttCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttcttc                                                                 <  1:832012/139‑1 (MQ=255)
                                                         cccTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCAt                                                              >  2:668529/1‑139 (MQ=255)
                                                                  cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCtt                                                                                                >  2:1179620/1‑96 (MQ=255)
                                                                  cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCtt                                                                                                <  1:1179620/96‑1 (MQ=255)
                                                                  cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTccc                                                               <  2:807227/129‑2 (MQ=255)
                                                                  cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCa                                                               >  1:807227/1‑129 (MQ=255)
                                                                  cATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACg                                                     >  2:1075539/1‑139 (MQ=255)
                                                                    tCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGt                                                   <  1:1075539/139‑1 (MQ=255)
                                                                       gATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCaa                                                <  1:445555/139‑1 (MQ=255)
                                                                        aTTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAg                                               >  2:564907/1‑139 (MQ=255)
                                                                              ctTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACg                                         <  1:564907/139‑1 (MQ=255)
                                                                                 cGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcag                                      <  1:660192/139‑1 (MQ=255)
                                                                                  gCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACgcagc                                     <  1:1107192/139‑1 (MQ=255)
                                                                                    tGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCAGACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   <  2:793765/139‑1 (MQ=255)
                                                                                      aaCAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTg                                 >  2:1243030/1‑139 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCAcc                                                                                                                          <  1:838315/45‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCAcc                                                                                                                          >  2:838315/1‑45 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgcg                                                                                                                     <  1:1041475/50‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACcgcgcg                                                                                                                     >  2:1041475/1‑50 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     >  2:443515/1‑66 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCgg                                                                                                     <  1:443515/66‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATg                                                                            >  2:1189237/1‑91 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATg                                                                            <  1:1189237/91‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTcc                                                                <  1:1016351/103‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTcc                                                                >  2:1016351/1‑103 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   >  1:543519/1‑132 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGcagcag                                   <  2:543519/132‑1 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                            >  2:869405/1‑139 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGcca                            >  2:420494/1‑139 (MQ=255)
                                                                                           gTCGCCGCCGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGC‑‑CACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGccacc                          >  2:56566/1‑139 (MQ=255)
                                                                                              gccgccGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttctt                                                                  >  2:1296196/1‑98 (MQ=255)
                                                                                              gccgccGTGCATGTCGATCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTttctt                                                                  <  1:1296196/98‑1 (MQ=255)
                                                                                                               tCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         >  1:1200577/1‑58 (MQ=255)
                                                                                                               tCATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGcaac                                                                                         <  2:1200577/58‑1 (MQ=255)
                                                                                                                cATTTCCAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCcg       <  2:39872/139‑1 (MQ=255)
                                                                                                                     ccAGCACTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTc  >  1:1010821/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                     
GGCTTCATAAGTTATGCGTATTCTCGTATCAGACCAGGCAGGGTAAACAGACACTTCCCCTGCCTGCATCCGATTACTCTTCGCTGAACAGGTCGCCGCCGTGCATGTCGATCATTTCCAGCGCTTTGCCGCCACCGCGCGCCACACAGGTCAGCGGGTCTTCAGCAACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTC  >  NC_000913/3399971‑3400226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A