Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	543,407	T→C	N214D (AAC→GAC)	allE ←	S‑ureidoglycine aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	543,407	0	T	C	100.0%	165.1 / NA	46	N214D (AAC→GAC)	allE	S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/23); total (23/23)

GTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGCATGTTCATATCAAAACCTAACTCTTTGGGCAGAAA  >  NC_000913/543273‑543539

gTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATaa                                                                                                                                  >  1:981956/1‑139 (MQ=255)

    cgGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAAccc                                                                                                                              >  2:369476/1‑139 (MQ=255)

              cTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGa                                                                                                                    <  1:121345/139‑1 (MQ=255)

              cTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGa                                                                                                                    <  2:96278/139‑1 (MQ=255)

               tttCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGGTGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGaa                                                                                                                   <  2:675932/139‑1 (MQ=255)

               tttCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGaa                                                                                                                   <  2:879771/139‑1 (MQ=255)

                                  aaCGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGtt                                                                                                >  1:320067/1‑139 (MQ=255)

                                    cGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGaa                                                                                                                   <  1:250722/118‑1 (MQ=255)

                                    cGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGaa                                                                                                                   >  2:250722/1‑118 (MQ=255)

                                      cTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTg                                                                                            <  1:886141/139‑1 (MQ=255)

                                         caccacGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAAc                                                                                         <  2:981956/139‑1 (MQ=255)

                                               gCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACgtgtgt                                                                                   <  2:90334/139‑1 (MQ=255)

                                                cccTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGtt                                                                                  >  2:640928/1‑139 (MQ=255)

                                                 ccTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGttt                                                                                 <  1:345862/139‑1 (MQ=255)

                                                       ccATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGatat                                                                           >  2:682774/1‑139 (MQ=255)

                                                          tAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGTGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAc                                                                        >  1:109627/1‑139 (MQ=255)

                                                           aaCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGttt                                                                                 <  1:262844/129‑1 (MQ=255)

                                                           aaCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGttt                                                                                 >  2:262844/1‑129 (MQ=255)

                                                           aaCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                                                                       <  2:751147/139‑1 (MQ=255)

                                                           aaCCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAAcc                                                                       >  2:606645/1‑139 (MQ=255)

                                                             ccAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGt                                                                     >  2:315351/1‑139 (MQ=255)

                                                                 ccTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggct                                                                 >  1:221322/1‑139 (MQ=255)

                                                                  cTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggctg                                                                >  2:171633/1‑139 (MQ=255)

                                                                  cTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGtggctg                                                                <  1:862439/139‑1 (MQ=255)

                                                                     tAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCa                                                             >  2:814843/1‑139 (MQ=255)

                                                                     tAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCa                                                             >  1:397291/1‑139 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCAcc                                                                                                     >  1:967670/1‑89 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCAcc                                                                                                     <  2:967670/89‑1 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCaa                                                   <  2:76124/139‑1 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCaa                                                   <  2:545229/139‑1 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCaa                                                   <  1:96240/139‑1 (MQ=255)

                                                                               gcgcCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCaa                                                   >  1:261910/1‑139 (MQ=255)

                                                                                   ccATAAAGATGTAATCGCCTTTTTTCACCGTGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAaga                                               <  2:109627/139‑1 (MQ=255)

                                                                                     aTAAAGATGTAATCTCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGTCCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGAGGCTGGCACCTGGTGCCAAagaga                                             <  1:646576/139‑1 (MQ=255)

                                                                                        aaGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGa                                          <  1:682774/139‑1 (MQ=255)

                                                                                             gTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGc                                     >  1:849204/1‑139 (MQ=255)

                                                                                             gTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTCGTGCAAAAGAGAGGATATGc                                     >  2:874640/1‑139 (MQ=255)

                                                                                                      ttttttCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGAcc                                                                                                                       >  1:935847/1‑48 (MQ=255)

                                                                                                      ttttttCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGAcc                                                                                                                       <  2:935847/48‑1 (MQ=255)

                                                                                                         tttCACCGGGATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCATCTGGTGCAAAAGAGAGGATATGCATGTTCATATCa                         <  1:846294/139‑1 (MQ=255)

                                                                                                                 ggATCCAGTTATTGTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGCATGTTCATATCAAAACCTaa                 >  1:57505/1‑139 (MQ=255)

                                                                                                                              gTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAaga                                               <  2:835682/96‑1 (MQ=255)

                                                                                                                              gTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAaga                                               >  1:835682/1‑96 (MQ=255)

                                                                                                                              gTCGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGCATGTTCATATCAAAACCTAACTCTTTGGGCAGa    >  2:610793/1‑139 (MQ=255)

                                                                                                                                cGAGGTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGCATGTTCATATCAAAACCTAACTCTTTGGGCAGaaa  >  1:364395/1‑139 (MQ=255)

                                                                                                                                   ggTCATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAgg                                           <  2:469451/95‑1 (MQ=255)

GTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCTGTAAAGAATAAGCGCCCATAAAGATGTAATCGCCTTTTTTCACCGGGATCCAGTTATTGTCGAGGTTATAAACCCCCTGACCGGAAAGAATATAGGCACCGTGTTCCTGAACGTGTGTTTCGATATAACCGTGGCTGGCACCTGGTGCAAAAGAGAGGATATGCATGTTCATATCAAAACCTAACTCTTTGGGCAGAAA  >  NC_000913/543273‑543539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A