Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
715 |
38.4 |
1606192 |
96.6% |
1551581 |
120.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
544,754 |
C→T |
R180H (CGT→CAT) |
allC ← |
allantoate amidohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 544,754 | 0 | C | T | 100.0%
| 96.6
/ NA
| 31 | R180H (CGT→CAT) | allC | allantoate amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/8); total (23/8) |
CCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA > NC_000913/544641‑544887
|
ccGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAag > 1:275622/1‑139 (MQ=255)
acgacgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAAt > 1:737119/1‑139 (MQ=255)
cgCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGc > 1:385090/1‑139 (MQ=255)
gCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCa < 2:708391/139‑1 (MQ=255)
caccacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACa < 2:737119/139‑1 (MQ=255)
cacGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACaaaa > 1:680765/1‑139 (MQ=255)
tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt < 2:99100/130‑1 (MQ=255)
tCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt > 1:99100/1‑130 (MQ=255)
cAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTc > 1:777042/1‑139 (MQ=255)
cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGGAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc < 1:353495/139‑1 (MQ=255)
cacacaGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGc > 2:455489/1‑139 (MQ=255)
aGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCAcgtc > 1:429025/1‑139 (MQ=255)
tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt < 1:684777/115‑1 (MQ=255)
tGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCAt > 2:684777/1‑115 (MQ=255)
tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc > 1:780323/1‑139 (MQ=255)
tatGCAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGc > 1:787572/1‑139 (MQ=255)
cAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc > 2:591066/1‑102 (MQ=255)
cAGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGc < 1:591066/102‑1 (MQ=255)
aGTTCAACAAAGGTTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 1:55818/1‑139 (MQ=255)
aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 1:294009/1‑139 (MQ=255)
aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 1:467376/1‑139 (MQ=255)
aGTTCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 2:637628/1‑139 (MQ=255)
tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAGTGCTGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGcc > 1:287909/1‑139 (MQ=255)
tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa > 2:762695/1‑139 (MQ=255)
tCAACAAAGGCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCaa > 1:111525/1‑139 (MQ=255)
gCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt > 2:284340/1‑139 (MQ=255)
gCTTTAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATAtttt > 1:372176/1‑139 (MQ=255)
tttAATATCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTa < 2:467376/139‑1 (MQ=255)
tCCTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGcccc < 2:429025/139‑1 (MQ=255)
ccTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa > 2:702507/1‑139 (MQ=255)
acTGATGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCa > 2:32003/2‑139 (MQ=255)
|
CCGTATAACGACGCTGCCCGACAATTGCATTCACCACGCCAATTGATTGCCCATTACTTTCCAGCACACAGCCCTGTTCAATATGCAGTTCAACAAAGGCTTTAATATCCTGACGCGGAGTTAG‑‑‑TGGGGCGTTCGGAAGAGTAAATCCGCAAGCCTTCATCGCATCGACAAAACTATTTCCTTTGGCATCACAGATATTCCGCACGTCGTCAGGATTCGCCAGCCCAAAAATATTTTTACTGCCCCA > NC_000913/544641‑544887
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
