Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	594,382	C→T	G130D (GGC→GAC)	cusS ←	copper‑sensing histidine kinase in two‑component regulatory system with CusR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	594,382	0	C	T	100.0%	146.6 / NA	46	G130D (GGC→GAC)	cusS	copper‑sensing histidine kinase in two‑component regulatory system with CusR
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/22); total (24/22)

AATCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGCCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAT  >  NC_000913/594248‑594517

aaTCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGa                                                                                                                                     >  2:160649/1‑139 (MQ=255)

 atcgatcgAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGaa                                                                                                                                    <  1:67264/139‑1 (MQ=255)

     atcgaAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGga                                                                                                                                >  1:102829/1‑139 (MQ=255)

                 aTGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGc                                                                                                                    <  1:93935/139‑1 (MQ=255)

                            ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  2:872120/1‑139 (MQ=255)

                            ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  2:317980/1‑139 (MQ=255)

                            ataAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCg                                                                                                         >  1:866798/1‑139 (MQ=255)

                                 tCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCttt                                                                                                    >  1:1003813/1‑139 (MQ=255)

                                   ggTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  >  2:977946/1‑139 (MQ=255)

                                    gTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATc                                                                                                 <  1:977946/139‑1 (MQ=255)

                                     tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATa                                                                                                                            <  2:964875/111‑1 (MQ=255)

                                     tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATa                                                                                                                            >  1:964875/1‑111 (MQ=255)

                                     tttGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCg                                                                                                >  2:185630/1‑139 (MQ=255)

                                                      ggCCAACCGGCAAGTTAATCATCCGCCAGTCGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCt                                                                                                      <  1:1010157/116‑1 (MQ=255)

                                                      ggCCAACCGGCAAGTTAATCATCCGCCAGTCGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCt                                                                                                      >  2:1010157/1‑116 (MQ=255)

                                                            ccGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTAACGCGTAAACTc                                                                         >  2:630751/1‑139 (MQ=255)

                                                                      aaTCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  >  1:839107/1‑104 (MQ=255)

                                                                      aaTCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTAt                                                                                                  <  2:839107/104‑1 (MQ=255)

                                                                        tcatcCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCgg                                                             <  1:795652/139‑1 (MQ=255)

                                                                            ccGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCa                                                         <  1:317980/139‑1 (MQ=255)

                                                                            ccGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCa                                                         >  1:308097/1‑139 (MQ=255)

                                                                             cGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCAc                                                        <  1:738045/139‑1 (MQ=255)

                                                                                cAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCgg                                                     <  2:102829/139‑1 (MQ=255)

                                                                                            tCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATaca                                         <  2:185728/139‑1 (MQ=255)

                                                                                                tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTcg                                                                        <  1:873790/104‑1 (MQ=255)

                                                                                                tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTcg                                                                        >  2:873790/1‑104 (MQ=255)

                                                                                                tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATc                                                                <  2:123583/112‑1 (MQ=255)

                                                                                                tatGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATc                                                                >  1:123583/1‑112 (MQ=255)

                                                                                                      cGTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTAcc                               <  1:433129/139‑1 (MQ=255)

                                                                                                       gTGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTAccc                              <  1:872120/139‑1 (MQ=255)

                                                                                                        tGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCt                             >  1:721147/1‑139 (MQ=255)

                                                                                                        tGACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCt                             >  2:247006/1‑139 (MQ=255)

                                                                                                        tGACCGTGGCCTGGCAGCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGCGTGATACACCGTTTTACCCt                             <  1:185630/139‑1 (MQ=255)

                                                                                                         gACCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTg                            >  1:393362/1‑139 (MQ=255)

                                                                                                          aCCGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGa                           >  1:51995/1‑139 (MQ=255)

                                                                                                            cGTGGCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACt                         >  1:700960/1‑139 (MQ=255)

                                                                                                               ggCCTGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATc                      <  2:916400/139‑1 (MQ=255)

                                                                                                                   tGGCATCATCATCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGcc                  >  1:564707/1‑139 (MQ=255)

                                                                                                                      catcatcatCGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAgg               <  2:393362/139‑1 (MQ=255)

                                                                                                                          atcatcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGaaa           >  2:880441/1‑139 (MQ=255)

                                                                                                                           tcatcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAAt          >  1:425541/1‑139 (MQ=255)

                                                                                                                             atcGTCGGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCa        <  1:160649/139‑1 (MQ=255)

                                                                                                                              tcgtcgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCaa       <  1:360929/139‑1 (MQ=255)

                                                                                                                               cgtcgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAac      >  1:762990/1‑139 (MQ=255)

                                                                                                                                  cgGGTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAAcaca   <  2:700960/139‑1 (MQ=255)

                                                                                                                                   gggTCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAt  <  2:308097/139‑1 (MQ=255)

AATCGATCGAAAGCGCGATGTAGAGCGTATAAATCGGTTTGCCGTCCACCAACGGGCCAACCGGCAAGTTAATCATCCGCCAGTTGCTGTGTTCCATATGCCCGTGACCGTGGCCTGGCATCATCATCGTCGGGCCGGAAAGGAGATACACCTCGCCACCCTGAGCGTCTTTATCGGGTATGGCGTCACGCGTAAACTCGCGGATATCCGGCGCACCGGGGGAGTGATACACCGTTTTACCCTGACTATCTGCCAGGGAAATCAACACAT  >  NC_000913/594248‑594517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A