Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	693,180	A→G	Y67H (TAT→CAT)	ybeZ ←	heat shock protein, putative NTPase, PhoH‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	693,180	0	A	G	95.1%	132.4 / ‑2.4	41	Y67H (TAT→CAT)	ybeZ	heat shock protein, putative NTPase, PhoH‑like protein
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (25/14); total (26/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

TATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATACAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGC  >  NC_000913/693048‑693314

tatTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCt                                                                                                                                  <  1:288983/139‑1 (MQ=255)

                   cTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCa                                                                                                               >  2:564812/1‑139 (MQ=255)

                      cGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGc                                                                                                                   <  2:517860/132‑1 (MQ=255)

                      cGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGc                                                                                                                   >  1:517860/1‑132 (MQ=255)

                        gCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGt                                                                                                          >  2:249675/1‑139 (MQ=255)

                           cGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGAc                                                                                                       >  2:538229/1‑139 (MQ=255)

                             ctctCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGc                                                                                                     >  2:965609/1‑139 (MQ=255)

                             ctctCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGc                                                                                                     >  2:500641/1‑139 (MQ=255)

                              tctcCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCa                                                                                                    >  1:149070/1‑139 (MQ=255)

                              tctcCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCa                                                                                                    >  2:569871/1‑139 (MQ=255)

                                  cgcgCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATc                                                                                                >  2:648903/1‑139 (MQ=255)

                                             ccAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGt                                                                                     <  1:281501/139‑1 (MQ=255)

                                             ccAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGt                                                                                     <  1:290007/139‑1 (MQ=255)

                                                  aCCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGtt                                                                                <  1:551632/139‑1 (MQ=255)

                                                          ttcttTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACgg                                                                                          <  1:718471/121‑1 (MQ=255)

                                                          ttcttTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGAATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACgg                                                                                          >  2:718471/1‑121 (MQ=255)

                                                            cttTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGtt                                                                      >  1:183306/1‑139 (MQ=255)

                                                                      aaGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTa                                                                                                                                 <  2:650455/70‑1 (MQ=255)

                                                                      aaGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTa                                                                                                                                 >  1:650455/1‑70 (MQ=255)

                                                                      aaGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGa                                                            >  1:949743/1‑139 (MQ=255)

                                                                      aaGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGa                                                            >  2:908864/1‑139 (MQ=255)

                                                                      aaGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATATCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGa                                                            >  2:95889/1‑139 (MQ=255)

                                                                       aGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGAt                                                           >  2:511810/1‑139 (MQ=255)

                                                                       aGGTGGATCTGTTCCGGTTCGATAGCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGAt                                                           >  2:714664/1‑139 (MQ=255)

                                                                             aTCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATATCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGAtct                                                     <  1:95889/139‑1 (MQ=255)

                                                                                   tCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGc                                               >  1:94776/1‑139 (MQ=255)

                                                                                   tCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGc                                               >  1:752807/1‑139 (MQ=255)

                                                                                       gTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCgag                                           >  1:1052597/1‑139 (MQ=255)

                                                                                       gTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCgag                                           >  1:829406/1‑139 (MQ=255)

                                                                                        ttCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCgaga                                          <  2:829406/139‑1 (MQ=255)

                                                                                                 cTGAATCTGACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGGCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCg                                 <  1:761889/139‑1 (MQ=255)

                                                                                                         gACCGCGCATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCtt                         >  2:211119/1‑139 (MQ=255)

                                                                                                             gcgcATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGAtg                     <  2:423095/139‑1 (MQ=255)

                                                                                                              cgcATCGGGGCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGAtgt                    >  1:834463/1‑139 (MQ=255)

                                                                                                              cgcATCGGGGCAGTATCGACATACAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTATAGTGGTTATCGCGGCGATTGATCTCGa                                                  >  2:468007/1‑109 (MQ=255)

                                                                                                              cgcATCGGGGCAGTATCGACATACAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGa                                                  <  1:468007/109‑1 (MQ=255)

                                                                                                                       gCAGTATCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCaa           <  2:149070/139‑1 (MQ=255)

                                                                                                                             tCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGa                                                                                             <  2:915925/51‑1 (MQ=255)

                                                                                                                             tCGACATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGa                                                                                             >  1:915925/1‑51 (MQ=255)

                                                                                                                                aCATGCAGGCTACGGAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGACACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGc  <  1:648903/139‑1 (MQ=255)

                                                                                                                                aCATGCAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGAAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGCTCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGc  >  2:695314/1‑139 (MQ=255)

TATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCAGTATCGACATACAGGCTACGCAGAATGTCTGCCGCAGCGGTGACGCAAATCGGACGGCCGGTCAGTTTAAAGTGGTTATCGCGGCGATTGATCTCGATGCCGAGACGGCGTTCGAGCTGCTTGATGTTGTCATCAAACGGGCCGC  >  NC_000913/693048‑693314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A