Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I2 R1 774 49.2 2112398 96.9% 2046913 118.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 896,680 A→G T183A (ACC→GCC)  potH → putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913896,6800AG100.0% 212.1 / NA 59T183A (ACC→GCC) potHputrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)

CCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAA  >  NC_000913/896553‑896815
                                                                                                                               |                                                                                                                                       
ccTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGcc                                                                                                                              >  2:401306/1‑139 (MQ=255)
  tGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGt                                                                                                                            >  2:288528/1‑139 (MQ=255)
  tGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGt                                                                                                                            >  2:564394/1‑139 (MQ=255)
             aCCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 <  1:1011675/139‑1 (MQ=255)
              ccTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCAt                                                                                                                <  2:414009/139‑1 (MQ=255)
              ccTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCAt                                                                                                                >  2:580414/1‑139 (MQ=255)
                 cGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 >  1:542046/1‑135 (MQ=255)
                 cGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCa                                                                                                                 <  2:542046/135‑1 (MQ=255)
                    ttCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGtt                                                                                                            <  2:273715/137‑1 (MQ=255)
                    ttCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGtt                                                                                                            >  1:273715/1‑137 (MQ=255)
                         aTCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCtt                                                                                                     <  2:342975/139‑1 (MQ=255)
                           ccGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTAc                                                                                                   >  1:545329/1‑139 (MQ=255)
                              cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:982803/1‑139 (MQ=255)
                              cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:911759/1‑139 (MQ=255)
                              cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  1:580414/139‑1 (MQ=255)
                              cgTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  2:982803/139‑1 (MQ=255)
                                   aTGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAAt                                                                                                                                    >  1:643345/1‑98 (MQ=255)
                                   aTGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAAt                                                                                                                                    <  2:643345/98‑1 (MQ=255)
                                             gggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACGATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  1:863702/139‑1 (MQ=255)
                                             gggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  2:833337/139‑1 (MQ=255)
                                              ggAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGc                                                                                <  2:323059/139‑1 (MQ=255)
                                               gAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGGATGCCAATCTGGCCGTTTATATCgg                                                                                                                   >  2:674679/1‑103 (MQ=255)
                                               gAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCgg                                                                                                                   <  1:674679/103‑1 (MQ=255)
                                                  tatTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGAt                                                                            <  1:216996/139‑1 (MQ=255)
                                                    ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  1:486831/117‑1 (MQ=255)
                                                    ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGAttt                                                                          <  1:401306/139‑1 (MQ=255)
                                                    ttAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACATg                                                                                                >  2:486831/1‑117 (MQ=255)
                                                     tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                <  2:148827/116‑1 (MQ=255)
                                                     tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTg                                                                                                >  1:148827/1‑116 (MQ=255)
                                                     tAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTta                                                                         >  1:67447/1‑139 (MQ=255)
                                                            caacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCGAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACcgcg                                                                  >  2:607589/1‑139 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTatat                                                                                                                      >  1:55382/1‑86 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTatat                                                                                                                      <  2:55382/86‑1 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGcc                                                                                              >  2:962684/1‑110 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGcc                                                                                              <  1:962684/110‑1 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGt                                                                 >  1:877038/1‑139 (MQ=255)
                                                             aacGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGt                                                                 >  2:55940/1‑139 (MQ=255)
                                                              acGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGtt                                                                >  2:236228/1‑139 (MQ=255)
                                                                 gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:917044/139‑1 (MQ=255)
                                                                 gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:911759/139‑1 (MQ=255)
                                                                 gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  1:55940/139‑1 (MQ=255)
                                                                 gtgtGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  1:564394/139‑1 (MQ=255)
                                                                    tgCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCg                                                          >  2:985887/1‑139 (MQ=255)
                                                                         aataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTg                                                     >  2:637924/1‑139 (MQ=255)
                                                                          ataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTa                                                                                         <  2:411309/102‑1 (MQ=255)
                                                                          ataatTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTa                                                                                         >  1:411309/1‑102 (MQ=255)
                                                                               tttCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               <  1:906299/139‑1 (MQ=255)
                                                                                     ctgTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCtgg                                         >  2:95390/1‑139 (MQ=255)
                                                                                                ggTTATCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATTTTGGCATTGTTTGCGCTTACGTGCCGTTTATGGTATTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAgcg                              <  2:258038/139‑1 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCt                                                                                                      <  1:1004125/63‑1 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCt                                                                                                      >  2:1004125/1‑63 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 <  1:797736/84‑1 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTg                                                                                 >  2:797736/1‑84 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               <  1:81143/118‑1 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGattatt                                               >  2:81143/1‑118 (MQ=255)
                                                                                                    aTCGATCAACCGCTGACCATTCTGCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTgg                          >  1:116735/1‑139 (MQ=255)
                                                                                                                           gCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             >  1:979283/1‑81 (MQ=255)
                                                                                                                           gCATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGAt                                                             <  2:979283/81‑1 (MQ=255)
                                                                                                                            cATGCCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGaaaa  <  1:95390/139‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
CCTGCCGTCGTGGACCTCGTTTCTGATCCGCGTTTATGCCTGGATGGGAATATTAAAAAACAACGGTGTGCTGAATAATTTTCTGCTGTGGCTGGGGGTTATCGATCAACCGCTGACCATTCTGCATACCAATCTGGCCGTTTATATCGGCATTGTTTACGCTTACGTGCCGTTTATGGTACTGCCGATTTATACCGCGTTGATTCGTATTGATTATTCGCTGGTGGAAGCAGCGCTGGATCTCGGTGCACGACCGCTGAAAA  >  NC_000913/896553‑896815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A