Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,032,528 |
G→T |
G130G (GGG→GGT) |
hyaA → |
hydrogenase 1, small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,032,528 | 0 | G | T | 100.0%
| 80.5
/ NA
| 27 | G130G (GGG→GGT) | hyaA | hydrogenase 1, small subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/11); total (16/11) |
TCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGGATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCCGCG > NC_000913/1032394‑1032645
|
tCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGt < 2:122414/139‑1 (MQ=255)
gccgccGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTg > 2:97752/1‑139 (MQ=255)
gCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTg > 2:518789/1‑139 (MQ=255)
ggAAGAAGTCTTTGAAGACATCATCACACAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGcc < 2:687341/139‑1 (MQ=255)
tGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGcc < 1:628116/139‑1 (MQ=255)
catcatCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGcgcg > 2:834590/1‑139 (MQ=255)
atcatcACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGa > 2:126435/1‑139 (MQ=255)
aaTACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTg > 2:1027130/1‑139 (MQ=255)
tACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTggg > 2:381352/1‑139 (MQ=255)
tACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTggg > 2:1008252/1‑139 (MQ=255)
aTGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAAc < 1:25592/139‑1 (MQ=255)
aaaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg < 1:834590/139‑1 (MQ=255)
aaaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg < 1:381352/139‑1 (MQ=255)
aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATcagcag < 1:821647/64‑1 (MQ=255)
aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATcagcag > 2:821647/1‑64 (MQ=255)
aaTATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcgc > 2:902391/1‑139 (MQ=255)
ccTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTgg > 1:950143/1‑139 (MQ=255)
cAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTg > 2:833173/1‑139 (MQ=255)
tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg < 1:607359/113‑1 (MQ=255)
tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg > 2:607359/1‑113 (MQ=255)
tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg > 2:147305/1‑139 (MQ=255)
tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg > 2:358610/1‑139 (MQ=255)
tCCGCCGCTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCcgcg < 1:1027130/139‑1 (MQ=255)
cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg < 1:988181/108‑1 (MQ=255)
cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg < 2:502697/108‑1 (MQ=255)
cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg > 1:502697/1‑108 (MQ=255)
cgcTGGGCGAGCAGGGTATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTgcg > 2:988181/1‑108 (MQ=255)
|
TCCCTCGATTACGACGATACTTTGATGGCTGCCGCCGGAACCCAGGCGGAAGAAGTCTTTGAAGACATCATCACGCAATACAATGGCAAATATATCCTCGCAGTAGAAGGTAATCCGCCGCTGGGCGAGCAGGGGATGTTCTGTATCAGCAGCGGTCGACCGTTTATTGAGAAACTCAAACGTGCCGCTGCCGGAGCCAGCGCGATTATCGCCTGGGGAACCTGCGCGTCCTGGGGCTGCGTGCAGGCCGCG > NC_000913/1032394‑1032645
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
