Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,266,086 |
C→G |
Q359E (CAG→GAG) |
prfA → |
peptide chain release factor RF‑1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,266,086 | 0 | C | G | 100.0%
| 109.8
/ NA
| 31 | Q359E (CAG→GAG) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (14/17); total (14/17) |
CGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACT > NC_000913/1265957‑1266204
|
ttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGaataat < 1:748529‑M2/13‑1 (MQ=255)
gatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATgg < 2:19677‑M2/15‑1 (MQ=255)
tgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATc < 1:592558‑M2/21‑1 (MQ=255)
gccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAac < 1:1007253‑M2/24‑1 (MQ=255)
ccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAaca < 1:169117‑M2/25‑1 (MQ=255)
ccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAaca < 2:226536‑M2/25‑1 (MQ=255)
ccaacgaaatcacgtcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAaca < 1:324186‑M2/25‑1 (MQ=255)
aatcacttcaacaggccgcccgtgacggtgataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGaa > 2:456780‑M2/108‑116 (MQ=255)
aatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacttcattttctttttttacagggtgGAGGAGGaa < 1:456780‑M2/9‑1 (MQ=255)
ttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGaa > 1:114748‑M2/102‑139 (MQ=255)
gccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATgg < 1:187793‑M2/15‑1 (MQ=255)
gccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATgg > 2:187793‑M2/94‑108 (MQ=255)
cggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAgg < 2:200823‑M2/57‑1 (MQ=255)
atattctgcgcgacagtctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGt > 2:488838‑M2/63‑139 (MQ=255)
tgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCt > 2:899667‑M2/57‑139 (MQ=255)
gcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTg > 2:501597‑M2/56‑139 (MQ=255)
cgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGa > 1:626231‑M2/55‑139 (MQ=255)
tctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCctgc < 1:488838‑M2/93‑1 (MQ=255)
cctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCctgc < 1:918203‑M2/93‑1 (MQ=255)
cgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTgg < 1:501597‑M2/96‑1 (MQ=255)
gtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTa < 2:228204‑M2/105‑1 (MQ=255)
acatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg > 1:883487‑M2/27‑102 (MQ=255)
acatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg < 2:883487‑M2/76‑1 (MQ=255)
acatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCaga > 2:919993‑M2/27‑139 (MQ=255)
catcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCagag > 1:75643‑M2/26‑139 (MQ=255)
catcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCagag > 1:326844‑M2/26‑139 (MQ=255)
cattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGc < 2:114748‑M2/117‑1 (MQ=255)
ttttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGt > 2:12405‑M2/21‑139 (MQ=255)
tctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACt > 1:93901‑M2/18‑139 (MQ=255)
ttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTa > 1:697132‑M2/11‑115 (MQ=255)
ttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTa < 2:697132‑M2/105‑1 (MQ=255)
|
CGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACT > NC_000913/1265957‑1266204
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
