Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
760 |
67.6 |
3323148 |
92.7% |
3080558 |
109.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,266,086 |
C→G |
Q359E (CAG→GAG) |
prfA → |
peptide chain release factor RF‑1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,266,086 | 0 | C | G | 100.0%
| 96.3
/ NA
| 29 | Q359E (CAG→GAG) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (11/18); total (11/18) |
GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218
|
taaccgcttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggag < 1:1078024‑M2/6‑1 (MQ=255)
atccatgcgccaacgaaatcacttcaacaggccgcccgtgaaggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGa < 1:602746‑M2/16‑1 (MQ=255)
catgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAata < 1:51111‑M2/19‑1 (MQ=255)
gccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAac < 1:1146022‑M2/24‑1 (MQ=255)
acaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGTAATAATGGAATATCAACACTGGTTACGTGAAGCaa < 2:1266097‑M2/42‑1 (MQ=255)
cgcccgtgacggggataacgatcgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatgattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGcc < 2:966146‑M2/48‑1 (MQ=255)
cccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCaa < 2:1327203‑M2/42‑1 (MQ=255)
cccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCaa > 1:1327203‑M2/90‑131 (MQ=255)
gtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACtt < 2:236777‑M2/53‑1 (MQ=255)
acggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAAATTCAg > 2:1111412‑M2/84‑139 (MQ=255)
ctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcgcg < 2:1118069‑M2/73‑1 (MQ=255)
ctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcgcg < 2:816057‑M2/73‑1 (MQ=255)
atattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGt < 1:825021‑M2/77‑1 (MQ=255)
gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATc > 2:678358‑M2/54‑74 (MQ=255)
gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATc < 1:678358‑M2/21‑1 (MQ=255)
gacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa > 2:447557‑M2/52‑138 (MQ=255)
gacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa < 1:447557‑M2/87‑1 (MQ=255)
gcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCctg < 2:122423‑M2/92‑1 (MQ=255)
gctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGc < 2:1594878‑M2/99‑1 (MQ=255)
gagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt < 1:409893‑M2/103‑1 (MQ=255)
gagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt > 2:409893‑M2/37‑139 (MQ=255)
cagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCgg > 1:1546032‑M2/31‑139 (MQ=255)
acatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACAATGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCaga > 2:1019984‑M2/27‑139 (MQ=255)
cattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGc > 1:1176264‑M2/23‑139 (MQ=255)
attttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCg < 1:1647848‑M2/118‑1 (MQ=255)
taaaaaaagagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg > 1:39115‑M2/16‑91 (MQ=255)
taaaaaaagagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg < 2:39115‑M2/76‑1 (MQ=255)
gggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTgg > 2:691643‑M2/6‑101 (MQ=255)
gggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTgg < 1:691643‑M2/96‑1 (MQ=255)
gtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCtt > 1:1146664‑M2/4‑139 (MQ=255)
|
GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
