Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,274,973 |
C→T |
T397M (ACG→ATG) |
ychO → |
putative invasin |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,274,973 | 0 | C | T | 100.0%
| 79.2
/ NA
| 26 | T397M (ACG→ATG) | ychO | putative invasin |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/12); total (14/12) |
TAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCAA > NC_000913/1274841‑1275108
|
tAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCggg > 2:608115/1‑139 (MQ=255)
tAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCggg > 1:859918/1‑139 (MQ=255)
tCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGcc < 2:375963/139‑1 (MQ=255)
cgccgTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGa > 1:156400/1‑139 (MQ=255)
ggATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTgg < 2:408251/139‑1 (MQ=255)
tCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACg < 1:608115/139‑1 (MQ=255)
ggCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGc > 1:94736/1‑139 (MQ=255)
cAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATGAAGTTTGATGCCGGGCGCACAAGCCAATAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTg > 1:636508/1‑139 (MQ=255)
taccGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGACCATGCCTGACTGGCa < 2:706358/137‑1 (MQ=255)
cGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGaa < 2:156400/139‑1 (MQ=255)
gAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACggg > 1:743506/1‑139 (MQ=255)
cAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg < 2:443746/139‑1 (MQ=255)
tCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaag > 2:177069/1‑139 (MQ=255)
tACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg < 1:278884/124‑1 (MQ=255)
tACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAgggg > 2:278884/1‑124 (MQ=255)
aTTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGt < 2:579324/139‑1 (MQ=255)
tCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGa < 1:969691/133‑1 (MQ=255)
tCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGa > 2:969691/1‑133 (MQ=255)
tGATTTGGCAGGGCGATATGCAGATATTAATTTGGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGCCGCTGATCATGCCTGACTGGCAGAAGGGGGAAGGAGCAAGCAATCACTGGCGATTGCCTgtggtggt < 2:950440/139‑1 (MQ=255)
ggCGATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAAcc > 1:778806/1‑139 (MQ=255)
gATACGCAGATATTAAGTTTGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAgg < 2:859918/139‑1 (MQ=255)
tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaagcaa < 1:595420/82‑1 (MQ=255)
tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGgcaagcaa > 2:595420/1‑82 (MQ=255)
tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa > 1:396250/1‑139 (MQ=255)
tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa > 1:344312/1‑139 (MQ=255)
tGATGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCaa > 2:85828/1‑139 (MQ=255)
|
TAACGGTGTTTCTGGCGACACCGCCGTGGGATCTAAAACCTGGCGAAACAGTGCCGCTGAAATTACAAATCCGCAGTCGTTACGGTATTCGGCAACTGATTTGGCAGGGCGATACGCAGATATTAAGTTTGACGCCGGGCGCACAAGCCAACAGCGCGGAGGGCTGGACGCTGATCATGCCTGACTGGCAGAACGGGGAAGGGGCAAGCAATCACTGGCGATTGTCTGTGGTGGTGGAAGATAACCAGGGGCAGCGTGTCTCCTCCAA > NC_000913/1274841‑1275108
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
