Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,362,857 |
A→G |
T39A (ACC→GCC) |
puuC → |
gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase, succinate semialdehyde dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,362,857 | 0 | A | G | 98.4%
| 218.0
/ NA
| 61 | T39A (ACC→GCC) | puuC | gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase, succinate semialdehyde dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (34/26); minor base C (1/0); total (35/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACG > NC_000913/1362725‑1362990
|
cGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTg > 2:139274/1‑139 (MQ=255)
gTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGa > 2:514478/1‑139 (MQ=255)
tGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGAt < 2:689834/139‑1 (MQ=255)
cAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGt < 1:308979/139‑1 (MQ=255)
ggAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCa > 2:784850/1‑139 (MQ=255)
gAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCAc > 1:801874/1‑139 (MQ=255)
aGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCAcc < 2:966751/139‑1 (MQ=255)
gTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCAGTTGATCCGGTCAccc > 1:1019356/1‑139 (MQ=255)
aTAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAgg > 2:451031/1‑139 (MQ=255)
aaTTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGc > 2:9578/1‑139 (MQ=255)
atcTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATAC‑GCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAAtt > 2:596382/1‑139 (MQ=255)
cTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTg > 1:103748/1‑139 (MQ=255)
gCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGccc > 1:664010/1‑139 (MQ=255)
gCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAag < 2:664010/139‑1 (MQ=255)
gCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAag < 2:103748/139‑1 (MQ=255)
aGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAagag > 1:957391/1‑139 (MQ=255)
gCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGata > 1:886495/1‑139 (MQ=255)
tAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGcc > 2:823049/1‑116 (MQ=255)
tAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGcc < 1:823049/116‑1 (MQ=255)
tAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCaa > 2:792141/1‑124 (MQ=255)
tAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCaa < 1:792141/124‑1 (MQ=255)
tAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGa > 2:903624/1‑139 (MQ=255)
aaGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGAc > 2:19981/1‑139 (MQ=255)
tGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGt > 1:1003371/1‑130 (MQ=255)
tGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGt < 2:1003371/130‑1 (MQ=255)
tatttattAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACgcgg < 1:100044/139‑1 (MQ=255)
aaCGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTg < 1:139274/139‑1 (MQ=255)
gTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAAcg > 2:567335/1‑139 (MQ=255)
aaTATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACgcgg > 2:995263/1‑139 (MQ=255)
aaTATACTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACgcgg > 1:995256/1‑139 (MQ=255)
tataCTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTg < 1:710519/73‑1 (MQ=255)
tataCTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTg > 2:710519/1‑73 (MQ=255)
tataCTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACgcggcg < 1:9578/139‑1 (MQ=255)
ataCTGCTGCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGa < 1:567335/139‑1 (MQ=255)
ctgctgCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTg < 1:204170/69‑1 (MQ=255)
ctgctgCGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTg > 2:204170/1‑69 (MQ=255)
tgctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAgcgc < 1:734581/108‑1 (MQ=255)
tgctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAgcgc > 2:734581/1‑108 (MQ=255)
tgctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTgg < 1:514478/139‑1 (MQ=255)
tgctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTgg > 1:315794/1‑139 (MQ=255)
cgctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTgg < 1:596382/138‑1 (MQ=255)
gctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGt > 1:517703/1‑139 (MQ=255)
gctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGt > 2:671856/1‑139 (MQ=255)
ctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTc < 2:580003/139‑1 (MQ=255)
ctgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTc < 2:803567/139‑1 (MQ=255)
tgcGGCGGAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCa > 1:458058/1‑139 (MQ=255)
tgcGGCGGAAAATGAAACCTTTGAAGCCGTCGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCa > 2:668674/1‑139 (MQ=255)
tgcGGCGGAAAATGAAACCTTTGAAGCCGCTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTgg > 1:150440/1‑136 (MQ=255)
tgcGGCGGAAAATGAAACCTTTGAAGCCGCTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTgg < 2:150440/136‑1 (MQ=255)
cggcggAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCAct > 1:818623/1‑139 (MQ=255)
cggcggAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCAGCGACTGGTCAct > 2:366397/1‑139 (MQ=255)
aggcggTAAATGAAACCTTTTAACCCGTTGAACCGGTCACCCAGGCACCGCTGGCGAAAATCGCCCGCGGCAAGAGCGTGTATCTCGACCGTGCGATGAGCCGAGCACGCGGGGTATTTGAACGCGGGGACTGGTCCct > 2:264969/2‑139 (MQ=255)
ggcggAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCACAGCACGCGGCGTATTTGAACGCGGCGACTGGTCActc < 1:19981/139‑1 (MQ=255)
gcggAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCAcg < 1:300533/107‑1 (MQ=255)
gcggAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCAcg > 2:300533/1‑107 (MQ=255)
gAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCAct > 1:299496/1‑134 (MQ=255)
gAAAATGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCAct < 2:299496/134‑1 (MQ=255)
aaaTGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACgcggcg > 2:672999/1‑122 (MQ=255)
aaaTGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACgcggcg < 1:672999/122‑1 (MQ=255)
aaTGAAACCTTTGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTtctc < 2:251478/139‑1 (MQ=255)
ttGAAGCCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACg < 2:801874/139‑1 (MQ=255)
|
CGTGAAACAGGAGTCATAATGAATTTTCATCATCTGGCTTACTGGCAGGATAAAGCGTTAAGTCTCGCCATTGAAAACCGCTTATTTATTAACGGTGAATATACTGCTGCGGCGGAAAATGAAACCTTTGAAACCGTTGATCCGGTCACCCAGGCACCGCTGGCGAAAATTGCCCGCGGCAAGAGCGTCGATATCGACCGTGCGATGAGCGCAGCACGCGGCGTATTTGAACGCGGCGACTGGTCACTCTCTTCTCCGGCTAAACG > NC_000913/1362725‑1362990
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
