Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I2 R1
|
774 |
49.2 |
2112398 |
96.9% |
2046913 |
118.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,533,129 |
C→T |
T26T (ACC→ACT) |
pptA → |
4‑oxalocrotonate tautomerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,533,129 | 0 | C | T | 100.0%
| 213.0
/ NA
| 65 | T26T (ACC→ACT) | pptA | 4‑oxalocrotonate tautomerase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (36/29); total (36/29) |
ACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAA > NC_000913/1532993‑1533261
|
acacATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa < 1:516887/139‑1 (MQ=255)
aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa > 1:157453/1‑135 (MQ=255)
aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGa < 2:157453/135‑1 (MQ=255)
aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt > 1:189930/1‑139 (MQ=255)
aTGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGtt > 2:1038418/1‑139 (MQ=255)
gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat > 1:612037/1‑139 (MQ=255)
gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat < 2:189930/139‑1 (MQ=255)
gACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat < 2:925623/139‑1 (MQ=255)
tgtgtgTTAATTTTACTGACATCTATCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCCCTTGCTGCAGATATTACTGACGTTATTATTCGTCATc > 2:276428/1‑139 (MQ=255)
tgtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTg > 1:195734/1‑139 (MQ=255)
gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 1:911022/139‑1 (MQ=255)
gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 2:1046232/139‑1 (MQ=255)
gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa > 2:947449/1‑139 (MQ=255)
gtgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 1:119142/139‑1 (MQ=255)
tgtTAATTTTACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGaa > 1:475260/1‑139 (MQ=255)
tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt > 2:85842/1‑125 (MQ=255)
tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCAt < 1:85842/125‑1 (MQ=255)
tACTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGa > 1:893670/1‑139 (MQ=255)
aCTGACATCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGAc > 2:805579/1‑139 (MQ=255)
aTCTTTCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACACAAAAGACAGTTCg > 2:98785/1‑139 (MQ=255)
tCAAAAGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAg > 1:454640/1‑139 (MQ=255)
aaaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCa < 2:9644/139‑1 (MQ=255)
aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCAt < 1:975152/139‑1 (MQ=255)
aaaGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGGTCGATAAGTAt < 2:612037/139‑1 (MQ=255)
aGGAGTGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTg < 2:263294/139‑1 (MQ=255)
agtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGctc > 1:350450/4‑139 (MQ=255)
gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGAt < 2:396141/125‑1 (MQ=255)
gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGAt > 1:396141/3‑127 (MQ=255)
gtgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGctct > 1:435678/3‑139 (MQ=255)
tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa > 2:986383/2‑107 (MQ=255)
tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 1:986383/106‑1 (MQ=255)
tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTTCTCTa > 2:865069/2‑139 (MQ=255)
tgTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTa > 2:477997/2‑139 (MQ=255)
tGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc < 2:340341/90‑1 (MQ=255)
tGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc > 1:340341/1‑90 (MQ=255)
gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 1:823712/98‑1 (MQ=255)
gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa > 2:823712/1‑98 (MQ=255)
gCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGAtt > 1:205677/1‑139 (MQ=255)
ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc > 1:133817/1‑88 (MQ=255)
ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc < 2:133817/88‑1 (MQ=255)
ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTc > 1:219866/1‑139 (MQ=255)
ccGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTc > 1:646504/1‑139 (MQ=255)
gCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCaa > 1:526044/1‑139 (MQ=255)
caTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat > 2:1004790/1‑78 (MQ=255)
caTCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGttat < 1:1004790/78‑1 (MQ=255)
cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc > 1:320227/1‑80 (MQ=255)
cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTc < 2:320227/80‑1 (MQ=255)
cGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGaa < 1:1038418/139‑1 (MQ=255)
aaTGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCa < 2:1048545/139‑1 (MQ=255)
tttCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTa > 2:196279/1‑139 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAAc < 2:615929/72‑1 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAAc > 1:615929/1‑72 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCaa < 1:615087/129‑1 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCaa > 2:615087/1‑129 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTggg > 2:317913/1‑139 (MQ=255)
cgTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTggg > 2:72720/1‑139 (MQ=255)
aaCTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGc > 1:243783/1‑139 (MQ=255)
gACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa > 1:763227/1‑61 (MQ=255)
gACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGa < 2:763227/61‑1 (MQ=255)
acaaAAAGCAGCACTTGCTGCAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGccc < 2:350450/139‑1 (MQ=255)
gctgcAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGa > 2:189757/1‑139 (MQ=255)
cAGATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATaaa < 1:177581/139‑1 (MQ=255)
gATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGa < 1:474470/139‑1 (MQ=255)
gATATTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGa < 1:72720/139‑1 (MQ=255)
tatTACTGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGaaa < 2:195734/139‑1 (MQ=255)
|
ACACATGACATTCACAATGTGTGTTAATTTTACTGACATCTTTCAAAAGGAGCGTAATCATGCCGCACATCGACATTAAATGTTTTCCGCGTGAACTGGACGAACAACAAAAAGCAGCACTTGCTGCAGATATTACCGACGTTATTATTCGTCATCTGAACAGTAAAGACAGTTCGATAAGCATTGCTCTACAGCAGATTCAACCAGAATCTTGGCAAGCTATCTGGGATGCCGAAATCGCGCCCCAAATGGAGGCTTTGATAAAGAAA > NC_000913/1532993‑1533261
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
