Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,093	G→A	L200L (CTG→TTG)	yeeO ←	putative multdrug exporter, MATE family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,093	0	G	A	100.0%	149.9 / NA	47	L200L (CTG→TTG)	yeeO	putative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (26/21); total (26/21)

AGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAGGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTC  >  NC_000913/2058957‑2059228

aGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGc                                                                                                                                       <  2:383054/139‑1 (MQ=255)

  cACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTa                                                                                                                                     >  1:975509/1‑139 (MQ=255)

     ccACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  <  1:845110/139‑1 (MQ=255)

                   cTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGc                                                                                                                                       <  2:934140/120‑1 (MQ=255)

                    tGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAG‑AAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTa                                                                                                                  <  1:185171/139‑1 (MQ=255)

                     gcgcCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTa                                                                                                                  >  2:482708/1‑139 (MQ=255)

                            tataACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGt                                                                                                           <  1:38500/139‑1 (MQ=255)

                               aaCGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTAtt                                                                                                        <  2:649846/139‑1 (MQ=255)

                                     aaaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGc                                                                                                  >  1:513989/1‑139 (MQ=255)

                                      aaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  <  2:652797/106‑1 (MQ=255)

                                      aaTGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCg                                                                                                                                  >  1:652797/1‑106 (MQ=255)

                                        tGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGcacc                                                                                               >  1:852241/1‑139 (MQ=255)

                                          gTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGcaccac                                                                                             >  2:697531/1‑139 (MQ=255)

                                                  cAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGcc                                                                                     <  2:393457/139‑1 (MQ=255)

                                                   aGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGGAGTCAAGAAATATGGGGATAATAATAATTATAAACATATGCCAGTTACGGTTAACAAATAGGGGTAGTTTTCTATTCCCTCCGCAACGAAGTGccc                                                                                    >  2:691350/1‑139 (MQ=255)

                                                   aGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGccc                                                                                    <  2:44123/139‑1 (MQ=255)

                                                          ccccGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTAcc                                                                             >  1:519040/1‑139 (MQ=255)

                                                          ccccGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTAcc                                                                             >  1:892469/1‑139 (MQ=255)

                                                                          tCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    <  1:349318/68‑1 (MQ=255)

                                                                          tCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    >  2:349318/1‑68 (MQ=255)

                                                                               gCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAgctgc                                                        >  1:964904/1‑139 (MQ=255)

                                                                                ccAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAgctgct                                                       <  2:16517/139‑1 (MQ=255)

                                                                                    gagaAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    <  1:1020357/58‑1 (MQ=255)

                                                                                    gagaAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTAc                                                                                                                                    >  2:1020357/1‑58 (MQ=255)

                                                                                         aaGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAAATg                                              >  2:1039356/1‑139 (MQ=255)

                                                                                              cGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTAc                                         >  1:85619/1‑139 (MQ=255)

                                                                                                 aaaTCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGt                                      >  2:661527/1‑139 (MQ=255)

                                                                                                    tCAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAg                                   <  2:892469/139‑1 (MQ=255)

                                                                                                     cAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGc                                  <  2:666772/139‑1 (MQ=255)

                                                                                                     cAATATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGc                                  <  2:551607/139‑1 (MQ=255)

                                                                                                      aaTATGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCt                                 <  1:661527/139‑1 (MQ=255)

                                                                                                       atatGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTCTTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGc                                                              <  2:745098/109‑1 (MQ=255)

                                                                                                       atatGCCGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGc                                                              >  1:745098/1‑109 (MQ=255)

                                                                                                            ccGCTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGAt                           <  2:513989/139‑1 (MQ=255)

                                                                                                               cTAATAATAATATTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAg                        >  1:357302/1‑139 (MQ=255)

                                                                                                                         tatTAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAg              <  1:482708/139‑1 (MQ=255)

                                                                                                                            tAAGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGc           >  1:218935/1‑139 (MQ=255)

                                                                                                                              aGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt         >  2:718690/1‑139 (MQ=255)

                                                                                                                              aGAATATTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGACAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCtt         >  1:455281/1‑139 (MQ=255)

                                                                                                                                  tatTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCa               <  2:475458/129‑1 (MQ=255)

                                                                                                                                  tatTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCa               >  1:475458/1‑129 (MQ=255)

                                                                                                                                   atTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACt    >  2:866288/1‑139 (MQ=255)

                                                                                                                                   atTCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACt    >  1:241718/1‑139 (MQ=255)

                                                                                                                                    ttCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCt                                                                                <  1:844323/62‑1 (MQ=255)

                                                                                                                                    ttCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCt                                                                                >  2:844323/1‑62 (MQ=255)

                                                                                                                                     tCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTc  >  1:513938/1‑139 (MQ=255)

                                                                                                                                     tCAAGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTc  >  2:316235/1‑139 (MQ=255)

AGCACCCACAAAATTGCAACTGCGCCAATATAACGAGAAATGGTTAAACCCAGCCCTGCCCCGACAAATCCCAGTCCCGGCCAGGAGAAAAGGCCGTAAATCAATATGCCGCTAATAATAATATTAAGAATATTCAGGCTACCGTTAATCAATAGCGGTATTTTCGTATTCCCTGCACCACGAAGTGCCCCGCTACCAATAAGAGTGATGGCAGCTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTC  >  NC_000913/2058957‑2059228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A