Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,864	T→C	S168G (AGT→GGT)	fruK ←	fructose‑1‑phosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,864	0	T	C	100.0%	147.1 / NA	41	S168G (AGT→GGT)	fruK	fructose‑1‑phosphate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/27); total (14/27)

GTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG  >  NC_000913/2261729‑2261985

gTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        >  1:768003/1‑139 (MQ=255)

 tAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCa                                                                                                                       >  1:453961/1‑139 (MQ=255)

    cgcATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAg                                                                                                                    >  1:878501/1‑139 (MQ=255)

      cATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGAt                                                                                                                  <  1:214619/139‑1 (MQ=255)

            cAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCa                                                                                                            <  2:154398/139‑1 (MQ=255)

              gCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAg                                                                                                          <  2:210033/139‑1 (MQ=255)

                 tCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGac                                                                                                       <  2:1048355/139‑1 (MQ=255)

                  cAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACAAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGaca                                                                                                      >  1:378991/1‑139 (MQ=255)

                    aTCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACt                                                                                                    <  1:164372/139‑1 (MQ=255)

                      cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGa                                                                                                  <  1:691107/139‑1 (MQ=255)

                      cacaTCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGa                                                                                                  <  2:240705/139‑1 (MQ=255)

                         aTCTTTCATTTCAGGC‑GTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTAc                                                                                              >  2:947811/1‑139 (MQ=255)

                              tcatttcaGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACAAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAg                                                                                          <  2:378991/139‑1 (MQ=255)

                               catttcaGGC‑GTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGc                                                                                        <  1:947811/139‑1 (MQ=255)

                                      ggCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATaa                                                                                                                <  2:509659/109‑1 (MQ=255)

                                      ggCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATaa                                                                                                                >  1:509659/1‑109 (MQ=255)

                                       gCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCAt                                                                                 >  2:398547/1‑139 (MQ=255)

                                        cAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATc                                                                                <  2:903110/139‑1 (MQ=255)

                                        cAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATc                                                                                <  2:768003/139‑1 (MQ=255)

                                         aGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATcc                                                                               <  2:1043034/139‑1 (MQ=255)

                                          gTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        >  2:560249/1‑97 (MQ=255)

                                          gTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATc                                                                                                                        <  1:560249/97‑1 (MQ=255)

                                                   cTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTCCGCAGGCGAGTCATCCAGTCGGTGaa                                                                     <  2:367209/139‑1 (MQ=255)

                                                   cTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGaa                                                                     <  2:976073/139‑1 (MQ=255)

                                                     gCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACg                                                                   >  1:411467/1‑139 (MQ=255)

                                                                     cgcgGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGcc                                                   <  2:922494/139‑1 (MQ=255)

                                                                         gcggTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGAc                                               <  2:878501/139‑1 (MQ=255)

                                                                                 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCt                                       <  2:942682/139‑1 (MQ=255)

                                                                                 gTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCACCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCt                                       <  2:322640/139‑1 (MQ=255)

                                                                                     cACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCg                                   <  2:449999/139‑1 (MQ=255)

                                                                                           ccACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGaca                             >  2:910261/1‑139 (MQ=255)

                                                                                                 tGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGAcc                       <  2:453961/139‑1 (MQ=255)

                                                                                                    cGCTTTCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    >  2:288686/1‑139 (MQ=255)

                                                                                                         tCAAACCTGCTACTAACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGaa               <  1:183997/139‑1 (MQ=255)

                                                                                                                  ctactaACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAg      >  2:779302/1‑139 (MQ=255)

                                                                                                                  ctactaACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAg      <  2:411467/139‑1 (MQ=255)

                                                                                                                      taACGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAg  <  1:67138/139‑1 (MQ=255)

                                                                                                                         cGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    <  2:246349/118‑1 (MQ=255)

                                                                                                                         cGCTTCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCata                    >  1:246349/1‑118 (MQ=255)

                                                                                                                             tCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGa                         >  1:956876/1‑109 (MQ=255)

                                                                                                                             tCACGGCTACCATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGa                         <  2:956876/109‑1 (MQ=255)

GTAGCGCATGTGCAGCTTCAATCACATCTTTCATTTCAGGCAGTTTACGGCCTGCCCAGATTTCCAGCTCGCGGCGGTTAGGTTTCACCAGCCACGGTGCCGCTTTCAAACCTGCTACTAACGCTTCACGGCTACTATCAAAGATAATGCAAGGACACTGACTACGCAGGCGAGTCATCCAGTCGGTGAACGCTTCCGGGCTGACGCCTGACGGTAAGCTTCCGCTGACACAGACCATATCGAACTGACCGAGCCAG  >  NC_000913/2261729‑2261985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A