Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,681	C→T	G529S (GGC→AGC)	eptC ←	LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,681	0	C	T	100.0%	173.9 / NA	55	G529S (GGC→AGC)	eptC	LPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/31); total (24/31)

ATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAC  >  NC_000913/4148550‑4148815

aTCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCaaa                                                                                                                                 >  2:731664/1‑139 (MQ=255)

  cGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATc                                                                                                                               >  2:983915/1‑139 (MQ=255)

      aTAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                                           <  1:868332/139‑1 (MQ=255)

               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  2:361541/1‑139 (MQ=255)

               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  1:954418/1‑139 (MQ=255)

               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  2:119294/1‑139 (MQ=255)

               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  1:448212/1‑139 (MQ=255)

               tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                                  >  2:727672/1‑139 (MQ=255)

                gtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                                 >  2:559812/1‑139 (MQ=255)

                gtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                                 <  2:122300/139‑1 (MQ=255)

                         tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATACGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCg                                                                                                        >  1:664823/1‑139 (MQ=255)

                           gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                      >  1:268437/1‑139 (MQ=255)

                           gATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGcc                                                                                                      <  1:488749/139‑1 (MQ=255)

                            aTCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTGGTTTCTTTGAACTGCGGATTCACCACTGAACAGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          >  1:649140/1‑118 (MQ=255)

                            aTCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                                          <  2:649140/118‑1 (MQ=255)

                             tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                             <  2:856360/130‑1 (MQ=255)

                             tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                             >  1:856360/1‑130 (MQ=255)

                              cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                                   <  1:983915/139‑1 (MQ=255)

                              cAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                                   <  2:763411/139‑1 (MQ=255)

                               aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  2:278602/139‑1 (MQ=255)

                                       tttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                                          <  1:225361/139‑1 (MQ=255)

                                        tacTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAATTCCGCCAGGCTGTATTTAc                                                                                         <  2:621271/137‑1 (MQ=255)

                                          cTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGa                                                                                       <  1:217554/139‑1 (MQ=255)

                                                   gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATcc                                                                              >  1:463660/1‑139 (MQ=255)

                                                    ttACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCt                                                                             >  1:232632/1‑139 (MQ=255)

                                                     tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                                 <  2:901205/102‑1 (MQ=255)

                                                     tACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGa                                                                                                                 >  1:901205/1‑102 (MQ=255)

                                                      aCCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGc                                                                           >  2:482912/1‑139 (MQ=255)

                                                                cGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCATGCGAGAAATCAc                                                                 >  2:472594/1‑139 (MQ=255)

                                                                 gggTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACg                                                                <  2:668328/139‑1 (MQ=255)

                                                                     aGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGa                                                            <  1:1043380/139‑1 (MQ=255)

                                                                                   gCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCa                                              >  1:770671/1‑139 (MQ=255)

                                                                                    cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCAt                                             >  1:594033/1‑139 (MQ=255)

                                                                                    cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCAt                                             >  1:1008538/1‑139 (MQ=255)

                                                                                         tCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAgg                                                                                                                       >  2:354506/1‑60 (MQ=255)

                                                                                         tCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAgg                                                                                                                       <  1:354506/60‑1 (MQ=255)

                                                                                             cacTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGAc                                    <  2:770671/139‑1 (MQ=255)

                                                                                                  aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCca                               <  1:361541/139‑1 (MQ=255)

                                                                                                            aTCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAAc                     >  2:377818/1‑139 (MQ=255)

                                                                                                               gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                  <  1:731664/139‑1 (MQ=255)

                                                                                                               gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                  <  1:358782/139‑1 (MQ=255)

                                                                                                               gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa                  <  2:594033/139‑1 (MQ=255)

                                                                                                                tgaCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGCCGTCCACAGCAGGAACGGaa                 <  2:620975/137‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:276516/139‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  2:857902/139‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:559812/139‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:482912/139‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  2:448212/139‑1 (MQ=255)

                                                                                                                tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:377818/139‑1 (MQ=255)

                                                                                                                     gtcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGgtg            <  2:268437/139‑1 (MQ=255)

                                                                                                                      tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 <  1:983888/133‑1 (MQ=255)

                                                                                                                      tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa                 >  2:983888/1‑133 (MQ=255)

                                                                                                                               aaGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  >  1:969685/1‑43 (MQ=255)

                                                                                                                               aaGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                                  <  2:969685/43‑1 (MQ=255)

                                                                                                                               aaGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAc  <  2:463660/139‑1 (MQ=255)

ATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGGTGTACATATGAC  >  NC_000913/4148550‑4148815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A