Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I1 R1	781	46.9	2231650	93.5%	2086592	109.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,678	G→A	L222L (CTG→CTA)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,678	0	G	A	100.0%	103.1 / NA	33	L222L (CTG→CTA)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/14); total (19/14)

TCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGG  >  NC_000913/4156557‑4156799

tCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                          >  2:891571/1‑139 (MQ=255)

        aTTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTc                                                                                                  >  2:515490/1‑139 (MQ=255)

                        ggCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTggg                                                                                                       <  1:411213/118‑1 (MQ=255)

                        ggCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTggg                                                                                                       >  2:411213/1‑118 (MQ=255)

                             cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                             >  2:161542/1‑139 (MQ=255)

                                  tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCAt                                                                        <  1:515490/139‑1 (MQ=255)

                                       aaaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGa                                                                   >  1:477385/1‑139 (MQ=255)

                                       aaaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGa                                                                   >  1:960019/1‑139 (MQ=255)

                                        aaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                 >  2:598991/1‑92 (MQ=255)

                                        aaTGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                 <  1:598991/92‑1 (MQ=255)

                                          tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGagca                                                                >  2:31507/1‑139 (MQ=255)

                                                    gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  1:547519/139‑1 (MQ=255)

                                                    gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  2:147091/139‑1 (MQ=255)

                                                                acTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                          <  2:29616/139‑1 (MQ=255)

                                                                      tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCg                                    >  2:347278/1‑139 (MQ=255)

                                                                       gAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGa                                   <  2:979146/139‑1 (MQ=255)

                                                                               cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      >  2:422544/1‑112 (MQ=255)

                                                                               cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  1:422544/112‑1 (MQ=255)

                                                                               cATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTAcg                           <  2:710542/139‑1 (MQ=255)

                                                                                      aCTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGAttt                    <  2:84097/139‑1 (MQ=255)

                                                                                         gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  1:840174/1‑139 (MQ=255)

                                                                                         gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  2:342752/1‑139 (MQ=255)

                                                                                         gACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAg                 >  1:211111/1‑139 (MQ=255)

                                                                                          aCGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGc                <  1:891571/139‑1 (MQ=255)

                                                                                            ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      >  1:609583/1‑99 (MQ=255)

                                                                                            ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                      <  2:609583/99‑1 (MQ=255)

                                                                                              cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa            >  1:183571/1‑139 (MQ=255)

                                                                                              cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATACCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATCCCGATGGATACGCGGATTTTCGCTTaa            >  2:727549/1‑139 (MQ=255)

                                                                                                        aaagtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTgg  >  2:1059377/1‑139 (MQ=255)

                                                                                                           gtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGttt                                                <  2:83706/90‑1 (MQ=255)

                                                                                                           gtgaaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGttt                                                >  1:83706/1‑90 (MQ=255)

                                                                                                              aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGAt                                  <  1:436812/101‑1 (MQ=255)

                                                                                                              aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGAt                                  >  2:436812/1‑101 (MQ=255)

TCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGG  >  NC_000913/4156557‑4156799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A