Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F1 I2 R1	777	59.2	2955938	92.5%	2734242	106.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,678	G→A	L222L (CTG→CTA)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,678	0	G	A	100.0%	148.6 / NA	46	L222L (CTG→CTA)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/22); total (24/22)

TGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCG  >  NC_000913/4156545‑4156808

tGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               <  1:1167599/139‑1 (MQ=255)

      ccGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         <  2:901145/139‑1 (MQ=255)

                                        aCGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTgg                                                                                       >  1:18843/1‑139 (MQ=255)

                                         cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                                      >  2:1040196/1‑139 (MQ=255)

                                         cGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGc                                                                                      >  1:1363737/1‑139 (MQ=255)

                                              tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               <  2:507947/93‑1 (MQ=255)

                                              tGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGc                                                                                                                               >  1:507947/1‑93 (MQ=255)

                                               gCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATg                                                                                <  2:1015263/139‑1 (MQ=255)

                                                      tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                                                     >  1:1032819/1‑127 (MQ=255)

                                                      tGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCg                                                                                     <  2:1032819/127‑1 (MQ=255)

                                                        aCCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGagcagc                                                                       >  2:445269/1‑139 (MQ=255)

                                                                gAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  2:608523/139‑1 (MQ=255)

                                                                     cAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGtt                                                          <  1:350015/139‑1 (MQ=255)

                                                                        aacaacTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  1:445269/139‑1 (MQ=255)

                                                                             cTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            <  2:633356/81‑1 (MQ=255)

                                                                             cTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            >  1:633356/1‑81 (MQ=255)

                                                                                  tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  2:767340/121‑1 (MQ=255)

                                                                                  tGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               >  1:767340/1‑121 (MQ=255)

                                                                                     gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                          >  1:1392298/1‑59 (MQ=255)

                                                                                     gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGccc                                                                                                                          <  2:1392298/59‑1 (MQ=255)

                                                                                     gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               <  1:11700/118‑1 (MQ=255)

                                                                                     gCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCa                                                               >  2:11700/1‑118 (MQ=255)

                                                                                         ggCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACg                                                     <  1:801608/124‑1 (MQ=255)

                                                                                         ggCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACg                                                     >  2:801608/1‑124 (MQ=255)

                                                                                                   cTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         <  2:1300978/46‑1 (MQ=255)

                                                                                                   cTGACGGCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCg                                                                                                                         >  1:1300978/1‑46 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            >  1:265882/1‑54 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGt                                                                                                            <  2:265882/54‑1 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       >  1:76788/1‑107 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  2:76788/107‑1 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       <  2:990132/139‑1 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:990132/1‑139 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:720754/1‑139 (MQ=255)

                                                                                                        ggCATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCtt                       >  1:1303841/1‑139 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                                   <  2:1123317/45‑1 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCt                                                                                                                   >  1:1123317/1‑45 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGc                                                                <  2:1187580/96‑1 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGc                                                                >  1:1187580/1‑96 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                                   <  2:187798/109‑1 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGt                                                   >  1:187798/1‑109 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa                     >  2:1041492/1‑139 (MQ=255)

                                                                                                          cATGATAGTGAAAGTGAACGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTCAACGGTATGCCGATGGGTACGCGGATTTTAGCTTaa                     >  2:923611/1‑139 (MQ=255)

                                                                                                                          aaCGCGGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAg     >  1:874772/1‑139 (MQ=255)

                                                                                                                            cgcgGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       >  2:330079/1‑87 (MQ=255)

                                                                                                                            cgcgGCGCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTaa                                                       <  1:330079/87‑1 (MQ=255)

                                                                                                                             gcggcgCTAGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGgcg  <  1:1041492/139‑1 (MQ=255)

TGCTCTCCGACGTCAGCGGCATTCTCGACGGCAAAGGGCAACGCATTGCCGAAATGACCGCCGCGAAAGCAGAACAACTGATTGAGCAGGGCATTATTACTGACGGCATGATAGTGAAAGTGAACGCGGCGCTGGATGCGGCCCGCACGCTGGGCCGTCCGGTAGATATCGCCTCCTGGCGTCATGCGGAGCAGCTTCCGGCACTGTTTAACGGTATGCCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCG  >  NC_000913/4156545‑4156808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A