Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,549,457	A→G	Y217C (TAC→TGC)	fimH →	minor component of type 1 fimbriae

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,549,457	0	A	G	100.0%	140.0 / NA	39	Y217C (TAC→TGC)	fimH	minor component of type 1 fimbriae
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/17); total (22/17)

TAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACA  >  NC_000913/4549323‑4549579

taatGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCctc                                                                                                                        >  1:633674/1‑139 (MQ=255)

                          gttGCGATGTTTCTGCTCGTGATGTCACTGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGcac                                                                                                                <  1:260577/119‑1 (MQ=255)

                          gcTGCGATGTTTCTGCTCGTGATGTCACTGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGcac                                                                                                                >  2:260577/1‑121 (MQ=255)

                                 tGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  2:633674/139‑1 (MQ=255)

                                                     ccGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGtt                                                                   <  2:789524/139‑1 (MQ=255)

                                                      cGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  1:632084/118‑1 (MQ=255)

                                                      cGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACAGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       >  2:632084/1‑118 (MQ=255)

                                                                   gACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAgg                                                     >  1:800633/1‑139 (MQ=255)

                                                                                  gTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGt                                      >  2:298834/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGt                                                 <  2:292243/127‑1 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGt                                                 >  1:292243/1‑127 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  2:21209/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:91185/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:857932/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:743939/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:258219/1‑139 (MQ=255)

                                                                                   tGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGtt                                     >  1:1003222/1‑139 (MQ=255)

                                                                                        aTTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGAcgc                                >  1:267994/1‑139 (MQ=255)

                                                                                            ctctTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCaa                            <  1:21209/139‑1 (MQ=255)

                                                                                               ttACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCCTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACgg                         <  2:743939/139‑1 (MQ=255)

                                                                                                tACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGt                        <  2:800633/139‑1 (MQ=255)

                                                                                                           gtgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTcc             >  1:663062/1‑139 (MQ=255)

                                                                                                            tgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCa            >  1:928708/1‑139 (MQ=255)

                                                                                                            tgCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCa            <  2:20301/139‑1 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCggg                                                                                                 <  1:598436/53‑1 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCggg                                                                                                 >  2:598436/1‑53 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       >  2:988599/1‑63 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGAtt                                                                                       <  1:988599/63‑1 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              >  2:113187/1‑120 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              <  1:113187/120‑1 (MQ=255)

                                                                                                             gCGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAg           >  2:106611/1‑139 (MQ=255)

                                                                                                              cGAATAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              <  1:508630/119‑1 (MQ=255)

                                                                                                              cGAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACgcgc                              >  2:508630/1‑119 (MQ=255)

                                                                                                               gAAAAGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCg         >  2:110364/1‑139 (MQ=255)

                                                                                                                aaaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGa        >  1:376625/1‑139 (MQ=255)

                                                                                                                 aaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGaa       <  1:386026/139‑1 (MQ=255)

                                                                                                                 aaaGCCAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGaa       <  1:110364/139‑1 (MQ=255)

                                                                                                                      cAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca  <  1:422692/139‑1 (MQ=255)

                                                                                                                      cAAAACCTGGGGTATTGCCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAaca  <  2:928708/139‑1 (MQ=255)

TAATGATGTGGTGGTGCCTACTGGCGGCTGCGATGTTTCTGCTCGTGATGTCACCGTTACTCTGCCGGACTACCCTGGTTCAGTGCCAATTCCTCTTACCGTTTATTGTGCGAAAAGCCAAAACCTGGGGTATTACCTCTCCGGCACAACCGCAGATGCGGGCAACTCGATTTTCACCAATACCGCGTCGTTTTCACCTGCACAGGGCGTCGGCGTACAGTTGACGCGCAACGGTACGATTATTCCAGCGAATAACA  >  NC_000913/4549323‑4549579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A