Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	774	49.2	2112398	96.9%	2046913	118.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,569,309	G→A	pseudogene (312/921 nt)	yjiP →	pseudogene, transposase_31 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,569,309	0	G	A	100.0%	220.0 / NA	68	pseudogene (312/921 nt)	yjiP	pseudogene, transposase_31 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/32); total (36/32)

CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGAAG  >  NC_000913/4569173‑4569444

cgcTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                                       <  1:167260/139‑1 (MQ=255)

  cTACAGTCCGCTATTTTAGAGTTGGTCAAGAGCCGCGAAGGTGATGGCTATATCTATGTGGTGATTGAAGATCAGAGCCGCGATGGCATTCATACGGCCTTTCGCCTGATGCGGTATTCCATGGCCGTGATGTAACGcc                                                                                                                                     <  2:636496/139‑1 (MQ=255)

        tCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATag                                                                                                                               <  1:298874/139‑1 (MQ=255)

            atatTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                                       >  1:993607/1‑127 (MQ=255)

            atatTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCa                                                                                                                           <  1:331680/139‑1 (MQ=255)

                  tATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATaa                                                                                                                     >  1:840624/1‑139 (MQ=255)

                    tGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAAc                                                                                                                   >  1:172685/1‑139 (MQ=255)

                      gTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                                       >  1:215814/1‑117 (MQ=255)

                      gTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                                       <  2:215814/117‑1 (MQ=255)

                        cGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCa                                                                                                               <  1:145310/139‑1 (MQ=255)

                        cGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGAAACGCCATATAGAGCATGATAAACGCCa                                                                                                               >  1:633455/1‑139 (MQ=255)

                         ggTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAg                                                                                                              >  2:828467/1‑139 (MQ=255)

                            aaaGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCg                                                                                                           <  1:1049392/139‑1 (MQ=255)

                                   cgcgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGt                                                                                                    >  1:475637/1‑139 (MQ=255)

                                            gATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATcc                                                                                           <  2:992613/139‑1 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCAtata                                                                                                                                >  2:533653/1‑88 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCAtata                                                                                                                                <  1:533653/88‑1 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCa                                                                                                               >  1:312789/1‑105 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCa                                                                                                               <  2:312789/105‑1 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                                       <  2:154107/129‑1 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                                       >  1:154107/1‑129 (MQ=255)

                                                           gtggtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGa                                                                                        <  1:692754/127‑1 (MQ=255)

                                                           gtggtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGa                                                                                        >  2:692754/1‑127 (MQ=255)

                                                           gtggtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATc                                                                            >  1:335770/1‑139 (MQ=255)

                                                              gtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACg                                                                         >  2:834128/1‑139 (MQ=255)

                                                               tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                                        >  2:813248/1‑139 (MQ=255)

                                                                gATTGAACATCAGAGCCGCGAGTACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                                       >  1:483289/1‑139 (MQ=255)

                                                                gATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                                       >  1:385362/1‑139 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCg                                                                                         >  1:319049/1‑120 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCg                                                                                         <  2:319049/120‑1 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTa                                                                      >  1:660121/1‑139 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTa                                                                      >  1:75838/1‑139 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTa                                                                      >  2:505101/1‑139 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTa                                                                      >  1:928671/1‑139 (MQ=255)

                                                                     aaCATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCg                                                                  <  1:828467/139‑1 (MQ=255)

                                                                            gagCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                                           <  2:385362/139‑1 (MQ=255)

                                                                             agCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                             >  2:53398/1‑120 (MQ=255)

                                                                             agCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                             <  1:53398/120‑1 (MQ=255)

                                                                             agCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCtt                                                          >  2:338206/1‑139 (MQ=255)

                                                                               ccGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTAc                                                        >  1:584282/1‑139 (MQ=255)

                                                                                 gcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCg                                                                  >  2:586689/1‑127 (MQ=255)

                                                                                 gcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCg                                                                  <  1:586689/127‑1 (MQ=255)

                                                                                  cgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTa                                                                >  1:1027823/1‑128 (MQ=255)

                                                                                  cgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTa                                                                <  2:1027823/128‑1 (MQ=255)

                                                                                        cATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTcc                                               <  1:338206/139‑1 (MQ=255)

                                                                                        cATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTcc                                               <  1:505101/139‑1 (MQ=255)

                                                                                         aTTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTccc                                              >  1:517654/1‑139 (MQ=255)

                                                                                                tGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                                   <  2:145520/127‑1 (MQ=255)

                                                                                                tGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                                   >  1:145520/1‑127 (MQ=255)

                                                                                                    cTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGcc                                                                                                                                     <  2:751883/41‑1 (MQ=255)

                                                                                                    cTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGcc                                                                                                                                     >  1:751883/1‑41 (MQ=255)

                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCa                                                                                                               <  2:629321/62‑1 (MQ=255)

                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCa                                                                                                               >  1:629321/1‑62 (MQ=255)

                                                                                                      ttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                                           <  2:563713/113‑1 (MQ=255)

                                                                                                      ttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                                           >  1:563713/1‑113 (MQ=255)

                                                                                                       tCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCg                                                                                                           <  2:870109/64‑1 (MQ=255)

                                                                                                       tCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCg                                                                                                           >  1:870109/1‑64 (MQ=255)

                                                                                                                gcgcTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGcc                       <  1:813248/139‑1 (MQ=255)

                                                                                                                           tGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTAc            <  2:584282/139‑1 (MQ=255)

                                                                                                                           tGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTAc            >  2:841852/1‑139 (MQ=255)

                                                                                                                              cGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTa                                                                <  2:929959/84‑1 (MQ=255)

                                                                                                                              cGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTa                                                                >  1:929959/1‑84 (MQ=255)

                                                                                                                                gTGATGTTACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCAc       >  1:918243/1‑139 (MQ=255)

                                                                                                                                gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCa                                                                                              <  1:729608/52‑1 (MQ=255)

                                                                                                                                gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCa                                                                                              >  2:729608/1‑52 (MQ=255)

                                                                                                                                gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCAc       >  2:273882/1‑139 (MQ=255)

                                                                                                                                gTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCAc       >  2:367603/1‑139 (MQ=255)

                                                                                                                                   aTGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGa    <  1:841852/139‑1 (MQ=255)

                                                                                                                                   aTGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGa    <  2:413830/139‑1 (MQ=255)

                                                                                                                                   aTGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGa    <  1:3562/139‑1 (MQ=255)

                                                                                                                                     gTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGAAg  <  1:273882/139‑1 (MQ=255)

                                                                                                                                     gTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGAAg  <  1:936836/139‑1 (MQ=255)

CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGACTACCGCACGGAAG  >  NC_000913/4569173‑4569444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A