Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,569,309	G→A	pseudogene (312/921 nt)	yjiP →	pseudogene, transposase_31 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,569,309	0	G	A	100.0%	124.1 / NA	39	pseudogene (312/921 nt)	yjiP	pseudogene, transposase_31 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/16); total (23/16)

CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGAC  >  NC_000913/4569173‑4569431

cgcTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          <  2:526534/139‑1 (MQ=255)

         ccGATATTTCATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATaga                                                                                                                 <  2:494777/139‑1 (MQ=255)

          cGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTACATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATagag                                                                                                                <  1:332291/139‑1 (MQ=255)

                      gTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGc                                                                                                    >  1:179886/1‑139 (MQ=255)

                         ggTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAg                                                                                                 >  1:606966/1‑139 (MQ=255)

                            aaaGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCg                                                                                              <  2:179886/139‑1 (MQ=255)

                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:216507/1‑139 (MQ=255)

                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:220260/1‑139 (MQ=255)

                                     cgAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTg                                                                                     >  1:32688/1‑139 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  2:478221/1‑139 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  1:377160/1‑139 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  2:327769/1‑139 (MQ=255)

                                                          tgtgGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTAt                                                                >  1:213713/1‑139 (MQ=255)

                                                              gtgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACg                                                            >  1:793704/1‑139 (MQ=255)

                                                               tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           <  2:377160/139‑1 (MQ=255)

                                                               tgATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           >  1:620740/1‑139 (MQ=255)

                                                                gATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  2:620740/139‑1 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                          <  2:256691/122‑1 (MQ=255)

                                                                 aTTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGAt                                                                          >  1:256691/1‑122 (MQ=255)

                                                                    gAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           >  2:105470/1‑134 (MQ=255)

                                                                    gAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACgg                                                           <  1:105470/134‑1 (MQ=255)

                                                                            gagCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCt                                              <  2:793704/139‑1 (MQ=255)

                                                                             agCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCtt                                             >  2:817004/1‑139 (MQ=255)

                                                                                cgcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  2:28442/1‑123 (MQ=255)

                                                                                cgcgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  1:28442/123‑1 (MQ=255)

                                                                                  cgAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTAcccc                                        >  2:525309/1‑139 (MQ=255)

                                                                                   gAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCt                                       <  1:478221/139‑1 (MQ=255)

                                                                                    aGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                      >  2:1707/1‑139 (MQ=255)

                                                                                    aGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTg                                      >  1:260090/1‑139 (MQ=255)

                                                                                             atatGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCtgtg                             <  1:525309/139‑1 (MQ=255)

                                                                                                  gCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                         >  1:147932/1‑90 (MQ=255)

                                                                                                  gCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTgctggc                        <  1:817004/139‑1 (MQ=255)

                                                                                                  gCCTTTCCCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATg                                                                         <  2:147932/90‑1 (MQ=255)

                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          <  2:571249/40‑1 (MQ=255)

                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          >  1:571249/1‑40 (MQ=255)

                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  1:643205/1‑104 (MQ=255)

                                                                                                   ccTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  2:643205/104‑1 (MQ=255)

                                                                                                    cTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTgctggctg                      >  2:416261/1‑139 (MQ=255)

                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          >  2:202811/1‑102 (MQ=255)

                                                                                                     tttCGCCTGATGCGCTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGt                                                          <  1:202811/102‑1 (MQ=255)

                                                                                                       tCGCCTGATGCGCTATTCCATGGCGGTGATGTAacg                                                                                                                          >  1:49551/1‑36 (MQ=255)

                                                                                                                   cTATTCCATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGccgac       >  1:95013/1‑139 (MQ=255)

                                                                                                                        ccATGGCGGTGATGTAACGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGccgacccgac  <  2:260090/139‑1 (MQ=255)

CGCTACACTCCGATATTTTATGGTCGGTAAAGACCCGCGAAGGCGATGGCTATATCTATGTGGTGATTGAACATCAGAGCCGCGAGGACATTCATATGGCCTTTCGCCTGATGCGCTATTCCATGGCGGTGATGTAGCGCCATATAGAGCATGATAAACGCCAGCCGCTACCGTTGGTCATCCCGATGCTATTTTATCACGGTAGCCGTAGTCCTTACCCCTGGTCCCTGTGCTGGCTGGACGAATTTGCCGACCCGAC  >  NC_000913/4569173‑4569431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A