Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,466,996 |
T→C |
pseudogene (1605/2513 nt) |
ydbA → |
pseudogene, autotransporter homolog, interrupted by IS2 and IS30 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,466,996 | 0 | T | C | 100.0%
| 171.1
/ NA
| 49 | pseudogene (1605/2513 nt) | ydbA | pseudogene, autotransporter homolog, interrupted by IS2 and IS30 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/25); total (24/25) |
CATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTT > NC_000913/1466873‑1467122
|
cATAGTCAAGAACGAAGGCGAGTGCACAATCACCAATGGCGGAACGGGCACTCAGATCAACGGTAACGGCACCACCGCGATTAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCggga < 2:1338837/139‑2 (MQ=255)
aTCGTCAATAACGAAGGCGAGAGCACCATCACCAACGGCGGCACCGGCACCCAGATCAACGGTGACGACGCCACCGCAAATAACAACGGAAAAA‑CCACTGTTGACGGAAAAGATTCCACCGGAACGGAAATCGCgggaa > 2:64878/1‑137 (MQ=11)
tCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCaa < 2:1315017/139‑1 (MQ=255)
aTAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg > 2:780817/1‑139 (MQ=255)
aTAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg < 1:780817/139‑1 (MQ=255)
tAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGCCGCCACCGCGAATAACAGTGGAAAAACCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg < 2:814411/139‑1 (MQ=255)
aTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa > 1:1047300/1‑138 (MQ=255)
aTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa < 2:1047300/138‑1 (MQ=255)
cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCg < 2:1283425/107‑1 (MQ=255)
cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCg > 1:1283425/1‑107 (MQ=255)
cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt > 1:1192407/1‑139 (MQ=255)
cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt > 2:1239037/1‑139 (MQ=255)
gcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc > 1:130008/1‑139 (MQ=255)
accggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGc < 2:1255783/96‑1 (MQ=255)
accggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGc > 1:1255783/1‑96 (MQ=255)
gcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCgg > 1:895009/1‑139 (MQ=255)
cacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGt < 2:346690/139‑1 (MQ=255)
cacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGt < 2:895009/139‑1 (MQ=255)
aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:130008/139‑1 (MQ=255)
aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 1:1239037/139‑1 (MQ=255)
aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:1192407/139‑1 (MQ=255)
aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:35035/139‑1 (MQ=255)
aTCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATg < 2:469054/139‑1 (MQ=255)
acgacgCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGaa > 2:644417/1‑139 (MQ=255)
gCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa > 1:451323/1‑103 (MQ=255)
gCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa < 2:451323/103‑1 (MQ=255)
aCCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:1322239/119‑1 (MQ=255)
aCCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa > 1:1035268/1‑119 (MQ=255)
aCCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:1035268/119‑1 (MQ=255)
aCCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa > 1:1322239/1‑119 (MQ=255)
tGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTga > 2:524888/1‑114 (MQ=255)
tGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTga < 1:524888/114‑1 (MQ=255)
gAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACAt < 1:771555/118‑1 (MQ=255)
gAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACAGTGGTGACAACGGCAAGGTTaa > 1:1056643/1‑139 (MQ=255)
gAAAAA‑CCACTGTTGATAGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGAATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACAt > 2:771555/1‑118 (MQ=255)
aa‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaacaac < 2:1059129/139‑1 (MQ=255)
a‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGt < 2:415158/93‑1 (MQ=255)
a‑CCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGt > 1:415158/1‑93 (MQ=255)
aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAg > 2:1288244/1‑139 (MQ=255)
aCTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAg > 2:661665/1‑139 (MQ=255)
tGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa > 1:1031408/1‑93 (MQ=255)
tGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa < 2:1031408/93‑1 (MQ=255)
ttGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATc < 1:762258/44‑1 (MQ=255)
ttGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATc > 2:762258/1‑44 (MQ=255)
aaaGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAttgttg > 1:1174637/1‑139 (MQ=255)
aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATgg < 2:1208913/54‑1 (MQ=255)
aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATgg > 1:1208913/1‑54 (MQ=255)
aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTgttgtt > 2:997097/1‑139 (MQ=255)
aCGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc < 2:282013/62‑1 (MQ=255)
aCGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc > 1:282013/1‑62 (MQ=255)
|
CATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAA‑CCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTT > NC_000913/1466873‑1467122
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
