Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 757 38.1 1607544 96.5% 1551279 117.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,466,996 T→C pseudogene (1605/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,466,9960TC100.0% 206.4 / NA 58pseudogene (1605/2513 nt)ydbApseudogene, autotransporter homolog, interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/30);  total (28/30)

CGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACT  >  NC_000913/1466861‑1467131
                                                                                                                                       |                                                                                                                                       
cGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCAcc                                                                                                                                      >  2:428296/1‑139 (MQ=255)
      ccAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAGAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATc                                                                                                                                <  1:673348/139‑1 (MQ=255)
          gCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAGGATTCCACGGGCACCAAAATCGCgg                                                                                                                            <  1:200626/139‑1 (MQ=255)
              tCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCaa                                                                                                                        >  2:700397/1‑139 (MQ=255)
                    aTAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                                  <  2:547044/139‑1 (MQ=255)
                       aCGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAAAGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCAt                                                                                                               <  2:255164/139‑1 (MQ=255)
                         gAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTg                                                                                                             >  2:133075/1‑139 (MQ=255)
                         gAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTg                                                                                                             <  1:381546/139‑1 (MQ=255)
                           aGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTa                                                                                                           >  1:376051/1‑139 (MQ=255)
                            ggCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTaa                                                                                                          <  1:14969/139‑1 (MQ=255)
                            ggCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTaa                                                                                                          <  1:428296/139‑1 (MQ=255)
                                   gCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGa                                                                                                   >  2:464613/1‑139 (MQ=255)
                                              aaTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                        <  1:700397/139‑1 (MQ=255)
                                              aaTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                        <  1:59011/139‑1 (MQ=255)
                                              aaTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                        <  1:394084/139‑1 (MQ=255)
                                               aTGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGAc                                                                                       >  1:546494/1‑139 (MQ=255)
                                                tGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACt                                                                                      >  2:586048/1‑139 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTGACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcgcg                                                                                                                             <  1:460873/99‑1 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATcgcg                                                                                                                             >  2:460873/1‑99 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTaa                                                                                                          >  2:345396/1‑118 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTaa                                                                                                          <  1:345396/118‑1 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTg                                                                                         >  2:247511/1‑135 (MQ=255)
                                                 ggcggcACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTg                                                                                         <  1:247511/135‑1 (MQ=255)
                                                   cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                                  >  2:94288/1‑108 (MQ=255)
                                                   cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCgg                                                                                                                  <  1:94288/108‑1 (MQ=255)
                                                   cggcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGtt                                                                                   >  2:116506/1‑139 (MQ=255)
                                                     gcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc                                                                                 >  1:707171/1‑139 (MQ=255)
                                                     gcaccggcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc                                                                                 <  2:238739/139‑1 (MQ=255)
                                                           gcacTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCgg                                                                           <  2:344127/139‑1 (MQ=255)
                                                                 aGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCa                                                                     <  1:121907/139‑1 (MQ=255)
                                                                  gATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCAt                                                                    <  1:464613/139‑1 (MQ=255)
                                                                             acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGaa                                                         >  1:690125/1‑139 (MQ=255)
                                                                             acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGaa                                                         >  2:502874/1‑139 (MQ=255)
                                                                                acgCCACTGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACAt                                                      >  2:98444/1‑139 (MQ=255)
                                                                                  gCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGCTGGTAGCCTGACTGTTAc                                                                                 >  2:551258/1‑110 (MQ=255)
                                                                                  gCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTAc                                                                                 <  1:551258/110‑1 (MQ=255)
                                                                                       cgcgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                        >  2:272547/1‑98 (MQ=255)
                                                                                       cgcgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGa                                                                                        <  1:272547/98‑1 (MQ=255)
                                                                                         cgAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACaa                                             <  1:316913/139‑1 (MQ=255)
                                                                                             tAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGc                                         <  1:571730/139‑1 (MQ=255)
                                                                                              aaCAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCa                                        <  1:98444/139‑1 (MQ=255)
                                                                                                cAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACg                                      >  1:725702/1‑139 (MQ=255)
                                                                                                   tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGtt                                   >  1:548234/1‑139 (MQ=255)
                                                                                                   tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGtt                                   <  2:725702/139‑1 (MQ=255)
                                                                                                   tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGtt                                   >  2:666976/1‑139 (MQ=255)
                                                                                                      aaaaaCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTg                                                                                                             <  2:394062/62‑1 (MQ=255)
                                                                                                      aaaaaCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTg                                                                                                             >  1:394062/1‑62 (MQ=255)
                                                                                                        aaaCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaacaa                              >  1:722282/1‑139 (MQ=255)
                                                                                                          aCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAacaaca                            >  1:299084/1‑139 (MQ=255)
                                                                                                          aCCACTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAacaaca                            <  2:707171/139‑1 (MQ=255)
                                                                                                              cTGTTGATGGAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAgg                        <  2:56200/139‑1 (MQ=255)
                                                                                                                      ggAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAttg                >  2:661361/1‑139 (MQ=255)
                                                                                                                      ggAAAAGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAttg                <  2:299084/139‑1 (MQ=255)
                                                                                                                           aGATTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTgttgtt           >  2:57077/1‑139 (MQ=255)
                                                                                                                             aTTCCACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc         <  1:176315/139‑1 (MQ=255)
                                                                                                                                 cACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGa                                                >  2:526420/1‑96 (MQ=255)
                                                                                                                                 cACGGGCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGa                                                <  1:526420/96‑1 (MQ=255)
                                                                                                                                    gggCACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTGACGATagt  >  1:586572/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CGGCGACCAGGCCATCGTCAATAACGAAGGCGAGAGCACTATCACCAATGGCGGCACCGGCACTCAGATCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACT  >  NC_000913/1466861‑1467131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A