Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,467,921 |
C→T |
*302* (TAG→TAA) |
insD1 ← |
IS2 transposase TnpB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,467,921 | 0 | C | T | 100.0%
| 101.8
/ NA
| 34 | *302* (TAG→TAA) | insD1 | IS2 transposase TnpB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/17); total (17/17) |
CTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT > NC_000913/1467793‑1468042
|
ctctGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa > 1:1104593/1‑139 (MQ=255)
ctctGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa < 2:1104593/139‑1 (MQ=255)
tATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa < 2:438544/139‑1 (MQ=255)
tAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc > 2:410172/1‑103 (MQ=255)
tAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc < 1:410172/103‑1 (MQ=255)
tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCa > 1:1252277/1‑139 (MQ=255)
aaTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc < 2:534560/111‑1 (MQ=255)
aaTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc > 1:534560/1‑111 (MQ=255)
gcgcTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg > 2:847508/1‑134 (MQ=255)
gcgcTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg < 1:847508/134‑1 (MQ=255)
gctacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga < 2:1252277/139‑1 (MQ=255)
tacgctgACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGt < 2:852954/84‑1 (MQ=255)
tacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGt > 1:852954/1‑84 (MQ=255)
aaCAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg > 1:476218/1‑139 (MQ=255)
cAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg < 2:476218/139‑1 (MQ=255)
gCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt > 1:1047900/1‑139 (MQ=255)
atTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg > 2:182159/1‑90 (MQ=255)
atTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg < 1:182159/90‑1 (MQ=255)
tGACAGTTTTGAACATCATGGGACATGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg > 2:144337/1‑126 (MQ=255)
tGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGGCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg < 1:144337/126‑1 (MQ=255)
ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa < 1:776786/48‑1 (MQ=255)
ttGAACATCATGGGACAAGAACCCTGGATATGCCCTTATATTTCCAGa > 2:776786/1‑48 (MQ=255)
tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa > 2:1142983/1‑47 (MQ=255)
tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa < 1:1142983/47‑1 (MQ=255)
tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCa > 2:1012766/1‑114 (MQ=255)
tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCGGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCa < 1:1012766/114‑1 (MQ=255)
catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt > 2:25239/1‑54 (MQ=255)
catGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt < 1:25239/54‑1 (MQ=255)
gACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa < 1:55849/139‑1 (MQ=38)
gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa < 1:887376/39‑1 (MQ=255)
gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa > 2:887376/1‑39 (MQ=14)
gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt < 1:902583/44‑1 (MQ=255)
gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct > 1:287212/1‑139 (MQ=18)
gAACCCTGGATTTACCCTTATATTTCCAGACATCTGTTATCACt > 2:902583/1‑44 (MQ=14)
|
CTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT > NC_000913/1467793‑1468042
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
