Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,645,063 |
G→A |
intergenic (‑161/+56) |
rem ← / ← hokD |
Qin prophage, uncharacterized protein/Qin prophage, small toxic polypeptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,645,063 | 0 | G | A | 100.0%
| 78.2
/ NA
| 26 | intergenic (‑161/+56) | rem/hokD | Qin prophage, uncharacterized protein/Qin prophage, small toxic polypeptide |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/12); total (14/12) |
CCCCTTTAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCGTTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCGGATTCGTACCTCGCAGA > NC_000913/1644929‑1645189
|
ccccTTTAGGGAACCTTCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGa < 1:503021/139‑1 (MQ=255)
cccTTTAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAg > 1:1237152/1‑139 (MQ=255)
cATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCcaca > 1:296294/1‑139 (MQ=255)
cATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCcaca > 2:297195/1‑139 (MQ=255)
ttATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCATCGTCCAGAACGTCAAACGCGTTGAAAATAAAAACAATAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtg < 2:191106/122‑1 (MQ=255)
ttATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtg > 1:191106/1‑122 (MQ=255)
gcgcTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGAt > 2:373335/1‑139 (MQ=255)
gcgcTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGAt > 1:340741/1‑139 (MQ=255)
tttGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtgcc < 2:31989/98‑1 (MQ=255)
tttGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtgcc > 1:31989/1‑98 (MQ=255)
ttGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGtc < 2:747344/139‑1 (MQ=255)
aTTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtgcctgc < 1:1064705/97‑1 (MQ=255)
aTTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtgcctgc > 2:1064705/1‑97 (MQ=255)
ggccAGAACTGCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtg < 2:1157679/74‑1 (MQ=38)
gTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGa > 2:208770/1‑74 (MQ=255)
gTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGa < 1:208770/74‑1 (MQ=255)
gTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGAtg > 1:1157679/1‑76 (MQ=255)
gTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGt > 1:1007949/1‑139 (MQ=255)
cgcgTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAg < 2:1007949/139‑1 (MQ=255)
aacaaaaacCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACa > 1:511917/1‑123 (MQ=255)
aacaaaaacCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACa < 2:511917/123‑1 (MQ=255)
aaacCCGCCGAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGtt < 1:373335/139‑1 (MQ=255)
gccgAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCg > 1:449757/1‑135 (MQ=255)
gccgAAGCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCg < 2:449757/135‑1 (MQ=255)
gCGGGTTAAGTGCGGGTGCATTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCGGATTCGTAcc > 2:246040/1‑139 (MQ=255)
tAAGTGCGGGTGCATTGAGGATGCCTGCC‑CATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCGGATTCGTACCTCGCaga < 2:1237152/139‑1 (MQ=255)
|
CCCCTTTAAGGGACCATCCCTCTTATCCCTGCGCGCTACTTAAGTATTTTTGATTCTATTCCGGCACCGTCCAGAACTTCAAACGCGTTGAAAATAAAAACAAAAACCCGCCGAAGCGGGTTAAGTGCGGGTGCGTTGAGGATGCCTGCCACATCAGAGGTGGCGAGGGATTTCTCCCCCGCCGGGTCTCTTACTCCTCAGGTTCGTAAGCTGTGAAGACAGCGACCTCCGTCTGGCCGGTTCGGATTCGTACCTCGCAGA > NC_000913/1644929‑1645189
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
