Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,842,141 A→G intergenic (‑6/‑230) ynjH ← / → gdhA DUF1496 family protein/glutamate dehydrogenase, NADP‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,842,1410AG100.0% 237.0 / NA 67intergenic (‑6/‑230)ynjH/gdhADUF1496 family protein/glutamate dehydrogenase, NADP‑specific
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/34);  total (33/34)

TGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCA  >  NC_000913/1842008‑1842260
                                                                                                                                     |                                                                                                                       
tGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCt                                                                                                                    <  1:631276/139‑1 (MQ=255)
tGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCt                                                                                                                    >  1:926268/1‑139 (MQ=255)
 gcgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGTTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCtt                                                                                                                   <  2:706629/139‑1 (MQ=255)
  cgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTCCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGTACTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGATCCTTa                                                                                                                  >  2:370104/1‑139 (MQ=255)
  cgcTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  1:618065/1‑139 (MQ=255)
  cgcTGACTACCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  1:665776/1‑139 (MQ=255)
          gCCCGCCTGAACTGAACTCTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAtt                                                                                                          <  1:370104/139‑1 (MQ=255)
              gCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGtct                                                                                                      <  1:1048256/139‑1 (MQ=255)
                 tgaactgaacACTTCTGGCTGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTg                                                                                                   <  2:665776/139‑1 (MQ=255)
                 tgaactgaacACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTg                                                                                                   <  1:845261/139‑1 (MQ=255)
                    actgaacACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                <  2:618065/139‑1 (MQ=255)
                                       aCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGt                                                                             >  1:112352/1‑139 (MQ=255)
                                       aCGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGt                                                                             >  2:216766/1‑139 (MQ=255)
                                                tCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTATGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACt                                                                    >  1:376504/1‑139 (MQ=255)
                                                    cGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAGATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAg                                                                >  2:293298/1‑139 (MQ=255)
                                                    cGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAg                                                                >  2:501990/1‑139 (MQ=255)
                                                        cGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  >  2:457811/1‑85 (MQ=255)
                                                        cGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTa                                                                                                                  <  1:457811/85‑1 (MQ=255)
                                                               tAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTa                                                     >  2:987833/1‑139 (MQ=255)
                                                               tAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTa                                                     <  1:216766/139‑1 (MQ=255)
                                                                     gggTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  1:501990/139‑1 (MQ=255)
                                                                     gggTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  1:987833/139‑1 (MQ=255)
                                                                       gTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCt                                                                                                 >  1:317740/1‑87 (MQ=255)
                                                                       gTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCt                                                                                                 <  2:317740/87‑1 (MQ=255)
                                                                              aGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATg                                      <  1:1342934/139‑1 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCa                                                                         >  1:204723/1‑102 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCa                                                                         <  2:204723/102‑1 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             <  1:141641/114‑1 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             >  2:141641/1‑114 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCt                                                           >  1:114991/1‑116 (MQ=255)
                                                                                tGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCt                                                           <  2:114991/116‑1 (MQ=255)
                                                                                 gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCa                                                >  1:92529/1‑126 (MQ=255)
                                                                                 gCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCa                                                <  2:92529/126‑1 (MQ=255)
                                                                                      tAATGGAGATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTAct                              <  1:293298/139‑1 (MQ=255)
                                                                                           gAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGt                         <  2:112352/139‑1 (MQ=255)
                                                                                             aaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAt                                                                                                           >  1:984394/1‑55 (MQ=255)
                                                                                             aaTGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTAt                                                                                                           <  2:984394/55‑1 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                >  1:268278/1‑53 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTa                                                                                                <  2:268278/53‑1 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTa                                                                 >  2:405889/1‑84 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTa                                                                 <  1:405889/84‑1 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             >  1:951759/1‑88 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGtt                                                      <  1:307658/95‑1 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGtt                                                      >  2:307658/1‑95 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               <  1:1240475/134‑1 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               >  2:1240475/1‑134 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  1:474405/1‑139 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  1:278551/1‑139 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTc          >  2:421467/1‑139 (MQ=255)
                                                                                                          cGGCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTCTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGt                                                             <  2:951759/88‑1 (MQ=255)
                                                                                                           ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAAc                                                                                                                <  2:282865/36‑1 (MQ=255)
                                                                                                           ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAAc                                                                                                                >  1:282865/1‑36 (MQ=255)
                                                                                                           ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               >  2:822922/1‑133 (MQ=255)
                                                                                                           ggCGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTg               <  1:822922/133‑1 (MQ=255)
                                                                                                             cGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               <  2:1216408/99‑1 (MQ=255)
                                                                                                             cGAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCAt                                               >  1:1216408/1‑99 (MQ=255)
                                                                                                              gAACAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTgg      <  2:376504/139‑1 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  1:14708/1‑116 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  1:372441/116‑1 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  2:372441/1‑116 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  2:14708/116‑1 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGttatta                    <  1:1258516/124‑1 (MQ=255)
                                                                                                               aaCAATGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGttatta                    >  2:1258516/1‑124 (MQ=255)
                                                                                                                  aaTGCTCGACTCACAGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCa  >  1:344847/1‑139 (MQ=255)
                                                                                                                              acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTg                                                          <  1:124419/71‑1 (MQ=255)
                                                                                                                              acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTg                                                          >  2:124419/1‑71 (MQ=255)
                                                                                                                              acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            >  2:509504/1‑101 (MQ=255)
                                                                                                                              acaGGGAGCTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTActct                            <  1:509504/101‑1 (MQ=255)
                                                                                                                                     |                                                                                                                       
TGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGGGTTAGCCAGTGCAATTAATGGAAATGCTAATACTACGGCGAACAATGCTCGACTCACAGGGAACTCCTTAACGTTATTGTCTCTGCTACTGATAACGGTAGCCGGGTGGCAAAACTTTAGCGTCTGAGTTATCGCATTTGGTTATGAGATTACTCTCGTTATTAATTTGCTTTCCTGGGTCA  >  NC_000913/1842008‑1842260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A