Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 765 53.2 2691310 93.4% 2513683 103.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,930,941 G→A G21S (GGT→AGT)  purT → phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,930,9410GA100.0% 194.4 / NA 60G21S (GGT→AGT) purTphosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/27);  total (33/27)

ATCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAT  >  NC_000913/1930811‑1931075
                                                                                                                                  |                                                                                                                                      
aTCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  1:671143/1‑136 (MQ=255)
aTCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  2:671143/136‑1 (MQ=255)
         aaaGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAaagaa                                                                                                                       >  1:480963/1‑139 (MQ=255)
                  cGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATc                                                                                                              <  1:482841/139‑1 (MQ=255)
                       aCGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAgtg                                                                                                         >  1:617611/1‑139 (MQ=255)
                                         cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTaga                                                                                       >  1:114261/1‑139 (MQ=255)
                                         cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTaga                                                                                       >  2:1299960/1‑139 (MQ=255)
                                            gcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGt                                                                                    <  2:617611/139‑1 (MQ=255)
                                                          gggATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  2:414037/78‑1 (MQ=255)
                                                          gggATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  1:414037/1‑78 (MQ=255)
                                                          gggATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATc                                                                      >  2:59841/1‑139 (MQ=255)
                                                               atTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTAt                                                                 >  1:1110848/1‑139 (MQ=255)
                                                                   gTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCg                                                             <  2:114261/139‑1 (MQ=255)
                                                                   gTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCg                                                             <  2:17723/139‑1 (MQ=255)
                                                                     tATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGa                                                            >  2:1110249/1‑138 (MQ=255)
                                                                     tATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGa                                                            <  1:1110249/138‑1 (MQ=255)
                                                                        gACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGtct                                                                                                >  1:269836/1‑99 (MQ=255)
                                                                        gACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGtct                                                                                                <  2:269836/99‑1 (MQ=255)
                                                                        gACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCa                                                        >  2:792060/1‑139 (MQ=255)
                                                                         aCGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCAc                                                       <  2:56923/139‑1 (MQ=255)
                                                                         aCGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCAc                                                       <  2:1110848/139‑1 (MQ=255)
                                                                          cGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  2:1040651/1‑62 (MQ=255)
                                                                          cGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  1:1040651/62‑1 (MQ=255)
                                                                               ttaGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCca                                                 >  1:397550/1‑139 (MQ=255)
                                                                                     aCTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCcatgcatg                                           >  1:666027/1‑139 (MQ=255)
                                                                                       tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGc                                                                                                                  <  2:206692/66‑1 (MQ=255)
                                                                                       tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGc                                                                                                                  >  1:206692/1‑66 (MQ=255)
                                                                                       tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTc                                         >  1:627881/1‑139 (MQ=255)
                                                                                       tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTc                                         >  1:26881/1‑139 (MQ=255)
                                                                                        gcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGcc                                                              <  1:985886/117‑1 (MQ=255)
                                                                                        gcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGcc                                                              >  2:985886/1‑117 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  2:457647/47‑1 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  1:457647/1‑47 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGAt                                                                       <  2:980907/107‑1 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGAt                                                                       >  1:980907/1‑107 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  2:535106/1‑139 (MQ=255)
                                                                                         cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  1:379169/1‑139 (MQ=255)
                                                                                          gctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgcg                                      <  1:450730/139‑1 (MQ=255)
                                                                                            tgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCa                                    >  2:1219858/1‑139 (MQ=255)
                                                                                                     cagcaACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCa                           <  2:26881/139‑1 (MQ=255)
                                                                                                         aaCTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTc                       <  1:792060/139‑1 (MQ=255)
                                                                                                               cgTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAat                 <  1:535106/139‑1 (MQ=255)
                                                                                                                       tattaGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGa         <  2:397550/139‑1 (MQ=255)
                                                                                                                            ggCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGt                        >  1:760457/1‑119 (MQ=255)
                                                                                                                            ggCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGt                        <  2:760457/119‑1 (MQ=255)
                                                                                                                             gCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGa         <  2:353823/133‑1 (MQ=255)
                                                                                                                             gCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGa         >  1:353823/1‑133 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCg                                                                                           <  2:167769/50‑1 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCg                                                                                           >  1:167769/1‑50 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGcc                                                              >  2:58889/1‑79 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGcc                                                              <  1:58889/79‑1 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGa                                                            >  1:1120590/1‑81 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGa                                                            <  2:1120590/81‑1 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTcc                             <  2:203273/112‑1 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTcc                             >  1:203273/1‑112 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCa                           <  2:1272584/114‑1 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCa                           >  1:1272584/1‑114 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:479519/1‑139 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  1:280300/1‑139 (MQ=255)
                                                                                                                              cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  1:1106288/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                      
ATCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAT  >  NC_000913/1930811‑1931075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A