Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
765 |
53.2 |
2691310 |
93.4% |
2513683 |
103.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,980,188 |
C→T |
*475* (TAG→TAA) |
otsA ← |
trehalose‑6‑phosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,980,188 | 0 | C | T | 100.0%
| 138.1
/ NA
| 44 | *475* (TAG→TAA) | otsA | trehalose‑6‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/25); total (19/25) |
TCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCA > NC_000913/1980055‑1980318
|
tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGAGAGATTAACCGCTCTTTCGc < 1:914124/139‑1 (MQ=255)
catTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCa < 1:18981/139‑1 (MQ=255)
gTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAg > 2:733855/1‑139 (MQ=255)
gATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGt < 1:1109851/139‑1 (MQ=255)
tttGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAg < 2:570826/139‑1 (MQ=255)
tcacaatcacaGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATc < 1:1310588/139‑1 (MQ=255)
gTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCgctg > 1:1093977/1‑134 (MQ=255)
gTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCgctg < 2:1093977/134‑1 (MQ=255)
tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCttt > 1:789739/1‑139 (MQ=255)
gcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt < 2:789739/139‑1 (MQ=255)
aGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg < 2:97200/139‑1 (MQ=255)
aGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg > 2:1128045/1‑139 (MQ=255)
aTCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg < 1:733855/139‑1 (MQ=255)
cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt < 1:529188/139‑1 (MQ=255)
aaaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCttt < 1:1128045/139‑1 (MQ=255)
tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc > 2:59643/1‑57 (MQ=255)
tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc < 1:59643/57‑1 (MQ=255)
tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg > 1:1035956/1‑139 (MQ=255)
aaGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa > 2:877791/1‑139 (MQ=255)
gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACt < 1:1331529/139‑1 (MQ=255)
aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGa > 1:37692/1‑54 (MQ=255)
aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGa < 2:37692/54‑1 (MQ=255)
aTGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGt > 2:1339204/1‑139 (MQ=255)
tgtgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTcc < 1:80399/131‑1 (MQ=255)
tgtgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTcc > 2:80399/1‑131 (MQ=255)
gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTgg < 1:630154/49‑1 (MQ=255)
gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTgg > 2:630154/1‑49 (MQ=255)
tGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa < 1:1006582/131‑1 (MQ=255)
tGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa > 2:1006582/1‑131 (MQ=255)
aGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa > 2:1330794/1‑102 (MQ=255)
aGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTa < 1:1330794/102‑1 (MQ=255)
ccACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa < 2:844334/123‑1 (MQ=255)
ccACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa > 1:844334/1‑123 (MQ=255)
ttACGGGAGATTGACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc < 2:1263386/109‑1 (MQ=255)
ttACGGGAGATTGACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc > 1:1263386/1‑109 (MQ=255)
ttACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc > 1:1293298/1‑109 (MQ=255)
ttACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc < 2:1293298/109‑1 (MQ=255)
gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATGGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt < 2:103973/82‑1 (MQ=255)
gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTAt > 1:103973/1‑82 (MQ=255)
gaTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa > 2:800934/1‑111 (MQ=255)
gaTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa < 1:800934/111‑1 (MQ=255)
aaCCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCa < 1:111531/139‑1 (MQ=255)
gCTCTTACGCAAGCTTTGGAAAGGCAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg > 2:1092563/1‑64 (MQ=255)
gCTCTTACGCAAGCTTTGGAAAGGCAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCgg < 1:1092563/64‑1 (MQ=255)
|
TCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCA > NC_000913/1980055‑1980318
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
