Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	765	53.2	2691310	93.4%	2513683	103.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,455	C→T	N409N (AAC→AAT)	sdaC →	putative serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,455	0	C	T	100.0%	182.9 / NA	57	N409N (AAC→AAT)	sdaC	putative serine transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (30/27); total (30/27)

ACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAT  >  NC_000913/2929321‑2929580

aCCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAt                                                                                                                           >  2:1071903/1‑139 (MQ=255)

     gAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGtt                                                                                                                      >  2:572265/1‑139 (MQ=255)

            aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGATGTCGTGa                                                                                                               >  2:1325832/1‑139 (MQ=255)

              cATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                             <  2:205469/139‑1 (MQ=255)

                 ccTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGt                                                                                                          <  1:432317/139‑1 (MQ=255)

                            ttGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAt                                                                                               >  1:938989/1‑139 (MQ=255)

                                 aCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          >  1:648315/1‑139 (MQ=255)

                                     tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                         >  2:1224540/1‑120 (MQ=255)

                                     tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                         <  1:1224540/120‑1 (MQ=255)

                                     tGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      <  2:938989/139‑1 (MQ=255)

                                     tGGGCGGCCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      >  2:1002605/1‑139 (MQ=255)

                                          ggTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtcttct                                                                                 <  2:648315/139‑1 (MQ=255)

                                            tCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAc                                                                               >  1:695065/1‑139 (MQ=255)

                                             ccAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAct                                                                              <  2:695065/139‑1 (MQ=255)

                                                      gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    <  1:196062/92‑1 (MQ=255)

                                                      gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    >  2:196062/1‑92 (MQ=255)

                                                             tCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    <  2:350414/85‑1 (MQ=255)

                                                             tCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGt                                                                                                                    >  1:350414/1‑85 (MQ=255)

                                                                      tGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgcg                                                     <  1:429184/139‑1 (MQ=255)

                                                                       gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActc                                                                             <  2:697623/114‑1 (MQ=255)

                                                                       gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActc                                                                             >  1:697623/1‑114 (MQ=255)

                                                                       gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  >  1:1088970/1‑125 (MQ=255)

                                                                       gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  <  2:1088970/125‑1 (MQ=255)

                                                                        aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                >  2:123219/1‑78 (MQ=255)

                                                                        aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                <  1:123219/78‑1 (MQ=255)

                                                                        aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActct                                                                            >  2:287388/1‑114 (MQ=255)

                                                                        aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTActct                                                                            <  1:287388/114‑1 (MQ=255)

                                                                        aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGcgcc                                                   >  1:114978/1‑139 (MQ=255)

                                                                          gCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGcgccgc                                                 >  2:532348/1‑139 (MQ=255)

                                                                            cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCtt                                               >  1:760031/1‑139 (MQ=255)

                                                                            cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCtt                                               >  1:669295/1‑139 (MQ=255)

                                                                            cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTACAATCTCCGCAATCTTCTActct                                                                            >  1:550490/1‑110 (MQ=255)

                                                                            cGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTACAATCTCCGCAATCTTCTActct                                                                            <  2:550490/110‑1 (MQ=255)

                                                                                        ttCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      <  1:210317/88‑1 (MQ=255)

                                                                                        ttCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                      >  2:210317/1‑88 (MQ=255)

                                                                                                       cAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGCGCGGCGCTTCCTCCGTTTTAGcg                    >  1:394917/1‑139 (MQ=255)

                                                                                                        aaTGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcgc                   <  2:114978/139‑1 (MQ=255)

                                                                                                           gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  <  1:602914/89‑1 (MQ=255)

                                                                                                           gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCt                                                                  >  2:602914/1‑89 (MQ=255)

                                                                                                           gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGAt                >  1:486646/1‑139 (MQ=255)

                                                                                                               aaGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt            <  1:532348/139‑1 (MQ=255)

                                                                                                                   aCAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAGCGCGATGTATTTcc        <  2:394917/139‑1 (MQ=255)

                                                                                                                    cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGCCTGATTGCAATCTCCGCAATCTTCTAGTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAATGCGATGTATTTCCt       <  1:817423/139‑1 (MQ=255)

                                                                                                                    cAGCGGTCACATCAGCAATGTATTCGTTGCCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCt       <  1:572265/139‑1 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGTATTCGTTGTCGTGAt                                                                                                              <  1:1338177/35‑1 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          >  2:1297241/1‑55 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                          <  1:1297241/55‑1 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgc                                                      >  1:1081830/1‑91 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgc                                                      <  2:1081830/91‑1 (MQ=255)

                                                                                                                     aGCGGTCACATCAGCAATGCATTCGTTGTCGTGAt                                                                                                              >  2:1338177/1‑35 (MQ=25)

                                                                                                                       cGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATg    >  1:850032/1‑139 (MQ=255)

                                                                                                                         gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                        <  1:1147753/53‑1 (MQ=255)

                                                                                                                         gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                        >  2:1147753/1‑53 (MQ=255)

                                                                                                                         gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAt  <  2:669295/139‑1 (MQ=255)

                                                                                                                         gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAt  >  2:860469/1‑139 (MQ=255)

                                                                                                                          tCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGgcggcg                                     >  1:1222286/1‑103 (MQ=255)

                                                                                                                          tCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGgcggcg                                     <  2:1222286/103‑1 (MQ=255)

ACCCTGAACCCGAGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGAT  >  NC_000913/2929321‑2929580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A