Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A11 F1 I1 R1 | 757 | 76.1 | 3598070 | 93.4% | 3360597 | 108.6 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,547,061 | C→T | A37T (GCA→ACA) | yddG ← | aromatic amino acid exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,547,061 | 0 | C | T | 100.0% | 192.7 / NA | 59 | A37T (GCA→ACA) | yddG | aromatic amino acid exporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/30); total (29/30) | |||||||||||
AGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTT > NC_000913/1546928‑1547196 | aGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGtgccgc > 2:839203/1‑139 (MQ=255) gcgcCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTgccgcc > 2:554774/1‑139 (MQ=255) agaTTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGcc < 1:1690073/139‑1 (MQ=255) tttCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAg < 2:833742/139‑1 (MQ=255) ttCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCTGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGc < 2:621778/139‑1 (MQ=255) gACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat < 1:279453/139‑1 (MQ=255) gACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat > 1:1527124/1‑139 (MQ=255) aCTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatc < 1:554774/139‑1 (MQ=255) tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGAcc < 2:1239646/107‑1 (MQ=255) tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGAcc > 1:1239646/1‑107 (MQ=255) tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatcaa < 1:839203/139‑1 (MQ=255) tCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaatcaa < 2:147832/139‑1 (MQ=255) aGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCa < 2:1527124/139‑1 (MQ=255) tAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATc > 1:831981/1‑139 (MQ=255) aaTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGt > 2:231594/1‑139 (MQ=255) aTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg < 2:1612397/139‑1 (MQ=255) aTAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg < 1:795000/139‑1 (MQ=255) ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat > 2:1526610/1‑116 (MQ=255) ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaat < 1:1526610/116‑1 (MQ=255) ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg < 1:426003/132‑1 (MQ=255) ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTg > 2:426003/1‑132 (MQ=255) ttCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCcaca > 2:1115396/1‑139 (MQ=255) ggAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc > 1:1673760/1‑114 (MQ=255) ggAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc < 2:1673760/114‑1 (MQ=255) ccAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg < 1:231594/139‑1 (MQ=255) aaGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCttttt > 1:1574213/1‑139 (MQ=255) aGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg < 1:873750/118‑1 (MQ=255) aGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg > 2:873750/1‑118 (MQ=255) tAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCATTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg > 2:609535/1‑114 (MQ=255) tAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg < 1:609535/114‑1 (MQ=255) gcagcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCAt > 2:1326436/1‑139 (MQ=255) cagcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATg > 1:1525232/1‑139 (MQ=255) agcCCGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGc < 1:1326436/139‑1 (MQ=255) cccGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCg > 2:97934/1‑38 (MQ=255) cccGCTTAATGAATAGATAGCAGCTGTGCCGCCGACCg < 1:97934/38‑1 (MQ=255) cTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg < 2:1394705/101‑1 (MQ=255) cTTAATGAATAGATAGCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAg > 1:1394705/1‑101 (MQ=255) tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCg < 1:727466/127‑1 (MQ=255) tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCg > 2:727466/1‑127 (MQ=255) tagatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGtctc > 1:1502409/1‑139 (MQ=255) agatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa > 2:1033718/1‑51 (MQ=255) agatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGaa < 1:1033718/51‑1 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc < 1:877597/70‑1 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTc > 2:877597/1‑70 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa > 1:955050/1‑80 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa < 2:955050/80‑1 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg < 2:1074432/101‑1 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCg > 1:1074432/1‑101 (MQ=255) gatagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGtctctc > 1:1184931/1‑139 (MQ=255) atagCAGCTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTctctct < 2:1184931/139‑1 (MQ=255) agcagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTa < 1:1751547/56‑1 (MQ=255) agcagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTa > 2:1751547/1‑56 (MQ=255) cagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa > 2:890031/1‑75 (MQ=255) cagcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGa < 1:890031/75‑1 (MQ=255) agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGa > 2:662041/1‑71 (MQ=255) agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGa < 1:662041/71‑1 (MQ=255) agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGt < 1:1735967/128‑1 (MQ=255) agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGt > 2:1735967/1‑128 (MQ=255) agcTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCg > 2:351026/1‑139 (MQ=255) cTGTGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGtt > 1:1599210/1‑139 (MQ=255) | AGCGCCAGACAGATTTCATAGCTGACGAATAACAGACTCCCGGCGAGTAAATAGCCTTTCGGGATTTGCCGAATACGCGGAAATCCAACCGTGAAGATTAACAGCAGCCCGCTTAATGAATAGATAGCAGCTGCGCCGCCGACCGGGCCGAGCCCCTCACTGACACCGCGAATCAATCCTACCATCGTGCTCCACAGGACGATCGCTATCAGCCCTATGAGCGTTGCTTTTTGTCGTGTCATGCTCGCTGTTTTGTCTCTCTTGCCGTT > NC_000913/1546928‑1547196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |