Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,993,949 T→C D253G (GAC→GGC)  uvrC ← excinuclease UvrABC, endonuclease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,993,9490TC100.0% 204.1 / NA 57D253G (GAC→GGC) uvrCexcinuclease UvrABC, endonuclease subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/28);  total (29/28)

CTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGT  >  NC_000913/1993818‑1994080
                                                                                                                                   |                                                                                                                                   
cTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtc                                                                                                                              <  2:762398/139‑1 (MQ=255)
         cGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGt                                                                                                                     >  1:1132241/1‑139 (MQ=255)
              aGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTgg                                                                                                                >  1:1773072/1‑139 (MQ=255)
                ttCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCg                                                                                                                       <  1:1122790/130‑1 (MQ=255)
                ttCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCg                                                                                                                       >  2:1122790/1‑130 (MQ=255)
                   cGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACg                                                                                                           >  2:514991/1‑139 (MQ=255)
                     tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGtcgtcg                                                                                                                          >  1:423264/1‑122 (MQ=255)
                     tACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGtcgtcg                                                                                                                          <  2:423264/122‑1 (MQ=255)
                      aCCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAAt                                                                                                        <  1:1067184/139‑1 (MQ=255)
                             ggCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                 <  1:1374479/139‑1 (MQ=255)
                             ggCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGttttt                                                                                                 >  2:1773557/1‑139 (MQ=255)
                                                tGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                            <  2:1231650/93‑1 (MQ=255)
                                                tGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGgtcgt                                                                                                                            >  1:1231650/1‑93 (MQ=255)
                                                  cGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  <  2:442683/133‑1 (MQ=255)
                                                  cGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  >  1:442683/1‑133 (MQ=255)
                                                      tGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAt                                                                        >  1:698724/1‑139 (MQ=255)
                                                          gAGCACTTTGCCCTGACGAATGAACAAGACGTGTACACAGGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTgg                                                                    <  1:514991/139‑1 (MQ=255)
                                                             cACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTcg                                                                 <  1:1339658/139‑1 (MQ=255)
                                                             cACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTcg                                                                 <  1:1773557/139‑1 (MQ=255)
                                                                  tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                        <  2:108572/79‑1 (MQ=255)
                                                                  tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGcc                                                                                                                        >  1:108572/1‑79 (MQ=255)
                                                                  tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGa                                                                                                               >  1:1343537/1‑88 (MQ=255)
                                                                  tGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGa                                                                                                               <  2:1343537/88‑1 (MQ=255)
                                                                                          ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTAt                                                                                                                   >  1:1613645/1‑60 (MQ=255)
                                                                                          ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTAt                                                                                                                   <  2:1613645/60‑1 (MQ=255)
                                                                                          ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                    >  2:1684781/1‑139 (MQ=255)
                                                                                          ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCCTCAAACTCCAg                                    >  2:1460620/1‑139 (MQ=255)
                                                                                          ggACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGCCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAg                                    >  1:529444/1‑139 (MQ=255)
                                                                                            acacAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAt                                  <  2:1781055/139‑1 (MQ=255)
                                                                                            acacAAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGAt                                  >  2:1551373/1‑139 (MQ=255)
                                                                                             cacaAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCCTCAAACTCCAGAtt                                 <  1:1460620/139‑1 (MQ=255)
                                                                                               caAGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTct                               >  2:1471828/1‑139 (MQ=255)
                                                                                                 aGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCa                                            <  1:1537299/124‑1 (MQ=255)
                                                                                                 aGCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCa                                            >  2:1537299/1‑124 (MQ=255)
                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAtt                                                                       >  2:534209/1‑96 (MQ=255)
                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAAtt                                                                       <  1:534209/96‑1 (MQ=255)
                                                                                                  gCCATGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggc                            >  2:832397/1‑139 (MQ=255)
                                                                                                      tGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                   >  1:310820/1‑112 (MQ=255)
                                                                                                      tGCCCGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGc                                                   <  2:310820/112‑1 (MQ=255)
                                                                                                         ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                     <  2:1531545/139‑1 (MQ=255)
                                                                                                         ccGCATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGt                     <  1:324591/139‑1 (MQ=255)
                                                                                                            cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  >  2:662940/1‑75 (MQ=255)
                                                                                                            cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCa                                                                                  <  1:662940/75‑1 (MQ=255)
                                                                                                            cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                         <  2:815732/132‑1 (MQ=255)
                                                                                                            cATCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTctggctgg                         >  1:815732/1‑132 (MQ=255)
                                                                                                             aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  2:1711611/1‑139 (MQ=255)
                                                                                                             aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  1:1747357/1‑139 (MQ=255)
                                                                                                             aTCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTcc                 >  1:1621425/1‑139 (MQ=255)
                                                                                                              tCGAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCa                <  2:698724/139‑1 (MQ=255)
                                                                                                                gAACGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATa              <  1:1711611/139‑1 (MQ=255)
                                                                                                                  aCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGtt                    <  1:346316/131‑1 (MQ=255)
                                                                                                                  aCGCCACACCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGtt                    >  2:346316/1‑131 (MQ=255)
                                                                                                                         acCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                         <  1:673687/39‑1 (MQ=255)
                                                                                                                         acCAATAACGCCGAGGTCGTCGCCGGTATTGGAAACGaa                                                                                                         >  2:673687/1‑39 (MQ=38)
                                                                                                                            aaTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGt  <  2:177234/139‑1 (MQ=255)
                                                                                                                             aTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                           <  1:1311521/81‑1 (MQ=255)
                                                                                                                             aTAACGCCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATa                                                           >  2:1311521/1‑81 (MQ=255)
                                                                                                                                   |                                                                                                                                   
CTACCACCTCGCTCAGTTCCGTACCGCCAGGCACTTTCGGGAAATAGCTGCGGCTGCCGAGCACTTTGCCCTGACGAATGAACAATACGTGGACACAAGCCATGCCCGCATCGAACGCCACACCAATAACGTCGAGGTCGTCGCCGGTATTGGAAACGAATTGTTTTTCGGTGACGCGTCGCACCGCCTGAATTTGGTCGCGAATACGTGCAGCTTCTTCAAACTCCAGATTCTGGCTGGCAGTTTCCATACGACTAATGAGT  >  NC_000913/1993818‑1994080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A