Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I1 R1 757 76.1 3598070 93.4% 3360597 108.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,358,163 G→A A228V (GCG→GTG)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,358,1630GA100.0% 219.6 / NA 68A228V (GCG→GTG) rhmA2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/29);  total (39/29)

CTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAA  >  NC_000913/2358034‑2358296
                                                                                                                                 |                                                                                                                                     
cTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCAtt                                                                                                                              >  1:17500/1‑139 (MQ=255)
     atatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  2:889851/139‑1 (MQ=255)
      tatCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTgcg                                                                                                                        <  2:17500/139‑1 (MQ=255)
                tACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAgg                                                                                                              >  1:108315/1‑139 (MQ=255)
                  ccTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAg                                                                                                            >  2:695397/1‑139 (MQ=255)
                    ttttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGcc                                                                                                          >  1:524619/1‑139 (MQ=255)
                      ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCcac                                                                                                        >  1:916946/1‑139 (MQ=255)
                      ttATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCcac                                                                                                        >  2:123039/1‑139 (MQ=255)
                             tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  2:549527/1‑139 (MQ=255)
                             tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  1:559433/1‑139 (MQ=255)
                             tGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGa                                                                                                 >  2:711161/1‑139 (MQ=255)
                                    tttttGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          >  2:1010576/1‑139 (MQ=255)
                                          ccTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          <  1:1542709/133‑1 (MQ=255)
                                          ccTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAg                                                                                          >  2:1542709/1‑133 (MQ=255)
                                           cTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCttt                                                                                   <  2:916946/139‑1 (MQ=255)
                                              aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  1:1021903/1‑139 (MQ=255)
                                              aTTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  2:953980/1‑139 (MQ=255)
                                                ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  2:524619/139‑1 (MQ=255)
                                                ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  1:700988/139‑1 (MQ=255)
                                                ttAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCg                                                                              <  1:713914/139‑1 (MQ=255)
                                                     cATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCgcagca                                                                         <  1:695397/139‑1 (MQ=255)
                                                        cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                                  >  2:1390264/1‑79 (MQ=255)
                                                        cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAgg                                                                                                                                  <  1:1390264/79‑1 (MQ=255)
                                                        cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:208232/88‑1 (MQ=255)
                                                        cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:208232/1‑88 (MQ=255)
                                                        cGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACgg                                                                      >  1:544632/1‑139 (MQ=255)
                                                         gCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGa                                                                     >  2:618444/1‑139 (MQ=255)
                                                         gCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGa                                                                     >  2:1254918/1‑139 (MQ=255)
                                                             gTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 <  1:123039/139‑1 (MQ=255)
                                                                        aGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTgg                                                      >  2:1246367/1‑139 (MQ=255)
                                                                         gggCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     >  1:748481/1‑139 (MQ=255)
                                                                              tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAGAGTTCGCTCCCCACAcc                                                                                                                                     <  1:1771555/54‑1 (MQ=255)
                                                                              tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                      >  1:1674922/1‑69 (MQ=255)
                                                                              tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGcc                                                                                                                      <  2:1674922/69‑1 (MQ=255)
                                                                              tCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaa                                                <  1:711161/139‑1 (MQ=255)
                                                                               cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1430682/65‑1 (MQ=255)
                                                                               cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1430682/1‑65 (MQ=255)
                                                                               cGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCaat                                               >  1:660520/1‑139 (MQ=255)
                                                                                       agCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCt                                       <  2:23628/139‑1 (MQ=255)
                                                                                         cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:863957/1‑55 (MQ=255)
                                                                                         cATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:863957/55‑1 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1402974/1‑52 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1040050/52‑1 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         >  2:1040050/1‑52 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATtgctgc                                                                                                                         <  1:1402974/52‑1 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                <  2:1243746/93‑1 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTAcc                                                                                >  1:1243746/1‑93 (MQ=255)
                                                                                            cGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACt                                  >  1:1281342/1‑139 (MQ=255)
                                                                                              tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  2:18240/1‑139 (MQ=255)
                                                                                              tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  1:988210/1‑139 (MQ=255)
                                                                                              tgtCAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTc                                >  1:1124361/1‑139 (MQ=255)
                                                                                                 cAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 >  2:807071/1‑103 (MQ=255)
                                                                                                 cAACGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATccg                                                                 <  1:807071/103‑1 (MQ=255)
                                                                                                   aCGCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGt                           >  2:187324/1‑139 (MQ=255)
                                                                                                     gCCAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGc                         <  2:988210/139‑1 (MQ=255)
                                                                                                      ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGcc                        <  1:618444/139‑1 (MQ=255)
                                                                                                      ccAACAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGcc                        <  1:1246367/139‑1 (MQ=255)
                                                                                                         aCAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCgg                     >  2:1706972/1‑139 (MQ=255)
                                                                                                          cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGc                    <  2:1124361/139‑1 (MQ=255)
                                                                                                          cAGCGACAAAGTTCGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGc                    <  1:1706972/139‑1 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACt                                                        <  1:951553/90‑1 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACt                                                        >  2:951553/1‑90 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     >  1:762352/1‑93 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGt                                                     <  2:762352/93‑1 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAg       >  1:526784/1‑139 (MQ=255)
                                                                                                                       cGCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAg       >  2:1500376/1‑139 (MQ=255)
                                                                                                                        gCTCCCCACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGc      >  2:891434/1‑139 (MQ=255)
                                                                                                                            cccACACCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCAGGTAGCCCaa  <  1:18240/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
CTTTGATATCAATAACTACCTTTTATGCGTGGCCCATTTTTGCCTGATTTAAACATCGCCAGTCGTTGATCCAGGGCATCGCTGTAGAGCATCGTGTCAACGCCAACAGCGACAAAGTTCGCTCCCCACGCCAGGCATTGCTGCGCCATATCAGGAGCCACAGCCAGAAAACCAGCCGCTTTACCCGCAGCACGGATCCGCCGAATACTGGTTTCAATAATTCGCTGCACTTCCGGGTGCCCGGCGTTATCCGGGTAGCCCAA  >  NC_000913/2358034‑2358296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A