Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	757	76.1	3598070	93.4%	3360597	108.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,386,872	C→T	A833A (GCG→GCA)	mscM ←	mechanosensitive channel protein, miniconductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,386,872	0	C	T	100.0%	226.3 / NA	69	A833A (GCG→GCA)	mscM	mechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (35/34); total (35/34)

GGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATC  >  NC_000913/4386740‑4387007

ggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGcactgcaccg                                                                                                                                   >  1:826835/1‑139 (MQ=255)

 gtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGa                                                                                                                                  <  2:855831/139‑1 (MQ=255)

    atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                               >  1:334283/1‑139 (MQ=255)

    atggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAggg                                                                                                                               >  1:716748/1‑139 (MQ=255)

      ggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTa                                                                                                                             >  1:707819/1‑139 (MQ=255)

            gatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGc                                                                                                                       >  1:229852/1‑139 (MQ=255)

                     aCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACtt                                                                                                              >  1:934407/1‑139 (MQ=255)

                     aCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACtt                                                                                                              >  1:597689/1‑139 (MQ=255)

                     aCCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACtt                                                                                                              >  1:1253544/1‑139 (MQ=255)

                        cGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                           <  2:229852/139‑1 (MQ=255)

                          tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcactgcacc                                                                                                                                    <  2:1646894/112‑1 (MQ=255)

                          tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAgcactgcacc                                                                                                                                    >  1:1646894/1‑112 (MQ=255)

                          tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc                                                                                                         >  1:192531/1‑139 (MQ=255)

                           aCCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                        <  1:886016/139‑1 (MQ=255)

                             ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                      <  1:1235898/139‑1 (MQ=255)

                                gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTg                                                                                                   >  1:480664/1‑139 (MQ=255)

                                         ccAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGa                                                                                          <  2:480664/139‑1 (MQ=255)

                                              tgctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa                                                                                     <  1:14337/139‑1 (MQ=255)

                                                   cAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCg                                                                                              <  2:334234/125‑1 (MQ=255)

                                                   cAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCg                                                                                              >  1:334234/1‑125 (MQ=255)

                                                   cAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATcc                                                                                >  2:722810/1‑139 (MQ=255)

                                                          gCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCg                                                                         <  2:716748/139‑1 (MQ=255)

                                                                       gcgcGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAg                                                            <  1:722810/139‑1 (MQ=255)

                                                                        cgcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAgg                                                           <  2:826835/139‑1 (MQ=255)

                                                                         gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                              >  2:1197875/1‑119 (MQ=255)

                                                                         gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                              <  1:1197875/119‑1 (MQ=255)

                                                                         gcgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGa                                                          >  1:491606/1‑139 (MQ=255)

                                                                          tgggCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCACCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGAGAAATATTTTCGAGGaa                                                         >  2:1106047/2‑139 (MQ=255)

                                                                          cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAg                                                                                                                  >  1:647270/1‑82 (MQ=255)

                                                                          cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAg                                                                                                                  <  2:647270/82‑1 (MQ=255)

                                                                          cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaa                                                                       >  1:1022228/1‑125 (MQ=255)

                                                                          cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGaa                                                                       <  2:1022228/125‑1 (MQ=255)

                                                                          cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGaa                                                         >  2:1614654/1‑139 (MQ=255)

                                                                               aaGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGa                                                    <  1:1614654/139‑1 (MQ=255)

                                                                               aaGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGa                                                    <  2:860059/139‑1 (MQ=255)

                                                                                    gcgcACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCg                                               <  2:762225/139‑1 (MQ=255)

                                                                                           aGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa                                        <  2:1253544/139‑1 (MQ=255)

                                                                                            gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                              <  1:1729041/100‑1 (MQ=255)

                                                                                            gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca                                                                              >  2:1729041/1‑100 (MQ=255)

                                                                                               gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                           >  1:1028851/1‑68 (MQ=255)

                                                                                               gCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                           <  2:1028851/68‑1 (MQ=255)

                                                                                                   ggtgATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGAc                                <  2:494990/139‑1 (MQ=255)

                                                                                                      gatgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACcac                             <  2:12556/139‑1 (MQ=255)

                                                                                                       atgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                                                                                                 >  2:1282230/1‑70 (MQ=255)

                                                                                                       atgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa                                                                                                 <  1:1282230/70‑1 (MQ=255)

                                                                                                        tgatAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAg                           <  2:334283/139‑1 (MQ=255)

                                                                                                          atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                         >  2:1796422/1‑139 (MQ=255)

                                                                                                          atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                         <  2:534486/139‑1 (MQ=255)

                                                                                                          atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                         <  1:1796422/139‑1 (MQ=255)

                                                                                                          atAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGca                         <  1:815629/139‑1 (MQ=255)

                                                                                                               cacCAGAATGGCAATCGGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATg                    >  2:1136340/1‑139 (MQ=255)

                                                                                                               cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                        >  1:1332289/1‑55 (MQ=255)

                                                                                                               cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg                                                                                                        <  2:1332289/55‑1 (MQ=255)

                                                                                                               cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATg                    >  1:639981/1‑139 (MQ=255)

                                                                                                                 ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                           <  1:718952/50‑1 (MQ=255)

                                                                                                                 ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt                                                                                                           >  2:718952/1‑50 (MQ=255)

                                                                                                                 ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGAc                  >  1:1040830/1‑139 (MQ=255)

                                                                                                                 ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGAc                  >  2:145321/1‑139 (MQ=255)

                                                                                                                 ccAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGAc                  <  2:491606/139‑1 (MQ=255)

                                                                                                                  cAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACa                 <  2:934407/139‑1 (MQ=255)

                                                                                                                   aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAgg                                                           >  2:684936/1‑96 (MQ=255)

                                                                                                                   aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAgg                                                           <  1:684936/96‑1 (MQ=255)

                                                                                                                   aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGAttt                                     >  2:682561/1‑118 (MQ=255)

                                                                                                                   aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGAttt                                     <  1:682561/118‑1 (MQ=255)

                                                                                                                   aGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAg                >  2:1752776/1‑139 (MQ=255)

                                                                                                                    gAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTcc                                                                                                                    >  2:749205/1‑38 (MQ=255)

                                                                                                                    gAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTcc                                                                                                                    <  1:749205/38‑1 (MQ=38)

                                                                                                                     aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                      <  2:440142/51‑1 (MQ=255)

                                                                                                                     aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc                                                                                                      >  1:440142/1‑51 (MQ=255)

                                                                                                                       tGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAg            >  1:407416/1‑139 (MQ=255)

                                                                                                                          cAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAg         >  2:221388/1‑139 (MQ=255)

                                                                                                                               aGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGa    >  2:131774/1‑139 (MQ=255)

                                                                                                                                 cactgcaccgAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCTACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATc  <  1:1136340/139‑1 (MQ=255)

GGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCGATC  >  NC_000913/4386740‑4387007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A