Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,386,872 |
C→T |
A833A (GCG→GCA) |
mscM ← |
mechanosensitive channel protein, miniconductance |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,386,872 | 0 | C | T | 100.0%
| 121.6
/ NA
| 38 | A833A (GCG→GCA) | mscM | mechanosensitive channel protein, miniconductance |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/19); total (19/19) |
GGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCG > NC_000913/4386740‑4387004
|
ggtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGcactgcaccg < 1:279530/139‑1 (MQ=255)
gtgatggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGa > 1:976803/1‑139 (MQ=255)
tggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGt > 2:235914/1‑139 (MQ=255)
tggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGt > 1:864018/1‑139 (MQ=255)
ggtggtgatggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTa > 1:719060/1‑139 (MQ=255)
ggCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTcc < 1:610401/139‑1 (MQ=255)
tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCTCTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc > 1:796366/1‑139 (MQ=255)
tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc > 2:537749/1‑139 (MQ=255)
tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc > 1:328035/1‑139 (MQ=255)
tACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc > 1:888568/1‑139 (MQ=255)
aCCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACg < 2:976803/139‑1 (MQ=255)
cccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGc < 2:302675/139‑1 (MQ=255)
ccGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGcc < 2:805509/139‑1 (MQ=255)
gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc < 1:377477/126‑1 (MQ=255)
gCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc > 2:377477/1‑126 (MQ=255)
aaaTCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCg < 2:541179/139‑1 (MQ=255)
aGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAgg < 2:888568/139‑1 (MQ=255)
gTGCCGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTg > 1:586042/1‑139 (MQ=255)
tgctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCAATGCACCGAGGGTAATTGGCGCCAGACTTTCTACGCCCTGTACCGTGGAGGTGa < 2:404302/139‑1 (MQ=255)
ctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGa < 1:793184/92‑1 (MQ=255)
ctgcAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGa > 2:793184/1‑92 (MQ=255)
cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa < 2:157049/99‑1 (MQ=255)
cgGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTa > 1:157049/1‑99 (MQ=255)
cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc < 1:105382/71‑1 (MQ=255)
cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGAc > 2:105382/1‑71 (MQ=255)
cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc > 2:660647/1‑78 (MQ=255)
cACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTAc < 1:660647/78‑1 (MQ=255)
gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca > 2:13685/1‑100 (MQ=255)
gCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCca < 1:13685/100‑1 (MQ=255)
aaCACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACaa < 1:235914/139‑1 (MQ=255)
aaCACCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACaa < 2:719060/139‑1 (MQ=255)
cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg < 2:382410/68‑1 (MQ=255)
cacCAGAATGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTgg > 1:382410/1‑68 (MQ=255)
aaTGGCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGaa > 1:570184/1‑139 (MQ=255)
gCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt > 2:592311/1‑42 (MQ=255)
gCAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCt < 1:592311/42‑1 (MQ=255)
cAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa > 1:884954/1‑108 (MQ=255)
cAATCAGCACTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGa < 2:884954/108‑1 (MQ=255)
cAGCTCTGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCg < 2:796366/139‑1 (MQ=255)
|
GGTGATGGTGGTGATGGCGTAACCCGTACCCGGCGTTAAATCCAGGTGCTGCAAAATCGCCAGTTCCAGCAGCGCGGGCAAGTTGCGCACCAGCTGCGTGGTGATGATAAACACCAGAATGGCAATCAGCACCGCACCGAGGGTAATTGGCTCCAGACTTTCTACGCCCTGTACCGTGGAGGTGACATCCCACAGCGAAATATTTTCGAGGAAGCCGAAAGCGGAATGGATTTCTGACCACAGCACAATGACAGAAAGCAGGGCG > NC_000913/4386740‑4387004
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
