Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	376,245	(G)_5→6	coding (787/1212 nt)	mhpT →	3‑hydroxyphenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	376,240	1	.	G	100.0%	120.3 / NA	34	M261R (ATG→AGG)	mhpT	3‑hydroxyphenylpropionic transporter
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (17/17); total (17/17)

CTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAAT‑GGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGT  >  NC_000913/376113‑376359

ctgctgTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCa                                                                                                               >  1:679603/1‑139 (MQ=255)

        gTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGAc                                                                                                       >  1:73740/1‑139 (MQ=255)

          tgtgTTATTTCTTCACTCAGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGt                                                                                                     <  2:679603/139‑1 (MQ=255)

              ttATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAAt                                                                                                 >  2:518519/1‑139 (MQ=255)

                     ttcACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgc                                                                                          <  2:73740/139‑1 (MQ=255)

                           ctgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCgg                                                                                                          <  2:610037/117‑1 (MQ=255)

                           ctgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCgg                                                                                                          >  1:610037/1‑117 (MQ=255)

                            tgctgGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGa                                                                                   >  1:405903/1‑139 (MQ=255)

                                      cTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCt                                                                         >  2:84208/1‑139 (MQ=255)

                                         cATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCg                                                                      <  1:84208/139‑1 (MQ=255)

                                         cATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCg                                                                      <  2:872413/139‑1 (MQ=255)

                                              tGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCggg                                                                                                         >  2:298302/1‑99 (MQ=255)

                                              tGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCggg                                                                                                         <  1:298302/99‑1 (MQ=255)

                                                aTCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGAGTCCAGCCATCGCAGGCGGCCGGGGGGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTa                                                               >  1:521097/1‑139 (MQ=255)

                                                   aaCTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAAcc                                                            >  1:88549/1‑139 (MQ=255)

                                                          tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     >  1:818871/1‑139 (MQ=255)

                                                          tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     <  2:537492/139‑1 (MQ=255)

                                                          tACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     <  2:821071/139‑1 (MQ=255)

                                                          tACCGCTACTTTTGGTGGAGCAAGGATTACAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGc                                                     >  2:557197/1‑139 (MQ=255)

                                                            ccGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         >  1:583583/1‑101 (MQ=255)

                                                            ccGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         <  2:583583/101‑1 (MQ=255)

                                                             cGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTAc                                                  >  2:400320/1‑139 (MQ=255)

                                                                            aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATTTTTGCACTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         >  2:675384/1‑85 (MQ=255)

                                                                            aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGgcg                                                                                         <  1:675384/85‑1 (MQ=255)

                                                                            aGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCa                                   >  1:353083/1‑139 (MQ=255)

                                                                               aaGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGTTACTGATTTATAGCTGCATGt                                <  1:557197/139‑1 (MQ=255)

                                                                                      ccAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTc                         <  2:540243/139‑1 (MQ=255)

                                                                                        aGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgc                       <  2:353083/139‑1 (MQ=255)

                                                                                            aTCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgctgct                   <  2:818871/139‑1 (MQ=255)

                                                                                            aTCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCgctgct                   <  1:518519/139‑1 (MQ=255)

                                                                                             tCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGctgctg                  <  1:400320/139‑1 (MQ=255)

                                                                                                             tGATGTTTGCCCTGCAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGgggt  >  2:91988/1‑139 (MQ=255)

                                                                                                                           cAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTc                                                                    >  2:459449/1‑59 (MQ=255)

                                                                                                                           cAAATGGGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTc                                                                    <  1:459449/59‑1 (MQ=255)

CTGCTGTGGTTGTGTTATTTCTTCACTCTGCTGGTGGTCTACATGTTGATCAACTGGCTACCGCTACTTTTGGTGGAGCAAGGATTCCAGCCATCGCAGGCGGCAGGGGTGATGTTTGCCCTGCAAAT‑GGGGGCGGCAAGCGGGACGTTAATGTTGGGCGCATTGATGGATAAGCTGCGTCCAGTAACCATGTCGCTACTGATTTATAGCGGCATGTTAGCTTCGCTGCTGGCGCTTGGAACGGTGT  >  NC_000913/376113‑376359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A