Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,204,160	G→A	113 (TAG→TAA)	ymfM →	e14 prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,204,160	0	G	A	100.0%	149.1 / NA	46	113 (TAG→TAA)	ymfM	e14 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/23); total (23/23)

ACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCATC  >  NC_000913/1204032‑1204294

aCTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCa                                                                                                                              >  2:952964/1‑139 (MQ=255)

       aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCa                                                                                                                        >  1:228383/1‑138 (MQ=255)

       aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCa                                                                                                                        <  2:228383/138‑1 (MQ=255)

       aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCAc                                                                                                                       >  1:339642/1‑139 (MQ=255)

       aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCAc                                                                                                                       >  1:75351/1‑139 (MQ=255)

         tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                    >  2:998827/1‑124 (MQ=255)

         tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                    <  1:998827/124‑1 (MQ=255)

         tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         >  2:636539/1‑135 (MQ=255)

         tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         <  1:636539/135‑1 (MQ=255)

         tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTa                                                                                                                     >  2:451599/1‑139 (MQ=255)

          gCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCACTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTaa                                                                                                                    >  2:886306/1‑139 (MQ=255)

                   agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                    <  2:575393/114‑1 (MQ=255)

                   agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAggt                                                                                                                                    >  1:575393/1‑114 (MQ=255)

                   agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  2:239767/139‑1 (MQ=255)

                   agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  1:808482/139‑1 (MQ=255)

                   agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCACTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  1:886306/139‑1 (MQ=255)

                     tCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  2:339642/139‑1 (MQ=255)

                               ggAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTaa                                                                                                               <  1:66113/123‑1 (MQ=255)

                               ggAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTaa                                                                                                               >  2:66113/1‑123 (MQ=255)

                                    cGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGtt                                                                                          >  1:677418/1‑139 (MQ=255)

                                      gAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgc                                                                                        <  1:878485/139‑1 (MQ=255)

                                       aaaCACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgcg                                                                                       <  1:451599/139‑1 (MQ=255)

                                                           cTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAgg                                                                                                                                     >  1:882777/1‑73 (MQ=255)

                                                           cTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAgg                                                                                                                                     <  2:882777/73‑1 (MQ=255)

                                                             ccGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         >  1:968033/1‑83 (MQ=255)

                                                             ccGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         <  2:968033/83‑1 (MQ=255)

                                                                cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         >  1:962371/1‑96 (MQ=255)

                                                                cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  2:962371/96‑1 (MQ=255)

                                                                cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAg                                                              >  2:438315/1‑139 (MQ=255)

                                                                             cATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATg                                                 <  1:438315/139‑1 (MQ=255)

                                                                               tGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGc                                               >  1:472151/1‑139 (MQ=255)

                                                                                 aaTGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         >  1:176282/1‑79 (MQ=255)

                                                                                 aaTGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  2:176282/79‑1 (MQ=255)

                                                                                   tGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         <  2:315071/61‑1 (MQ=255)

                                                                                   tGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGc                                                                                                                         >  1:315071/1‑61 (MQ=255)

                                                                                         aGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCt                                     <  2:75351/139‑1 (MQ=255)

                                                                                           cccGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                         >  2:344419/1‑117 (MQ=255)

                                                                                           cccGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                         <  1:344419/117‑1 (MQ=255)

                                                                                               cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                           <  2:493099/95‑1 (MQ=255)

                                                                                               cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                           >  1:493099/1‑95 (MQ=255)

                                                                                                         ttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCTGTAATGACg                     <  2:677418/139‑1 (MQ=255)

                                                                                                         ttCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                     <  2:472151/139‑1 (MQ=255)

                                                                                                                       aGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTgg       >  2:487323/1‑139 (MQ=255)

                                                                                                                         ggAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGcc     >  2:913958/1‑139 (MQ=255)

                                                                                                                            aaTAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCATc  >  1:385766/1‑139 (MQ=255)

                                                                                                                             aTAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCAt   <  2:323786/137‑1 (MQ=255)

ACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCATC  >  NC_000913/1204032‑1204294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A