Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,741,640 |
(T)6→7 |
coding (228/1149 nt) |
cfa → |
cyclopropane fatty acyl phospholipid synthase, SAM‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,741,634 | 1 | . | T | 100.0%
| 118.5
/ NA
| 38 | M74I (ATG→ATT) | cfa | cyclopropane fatty acyl phospholipid synthase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (18/20); total (18/20) |
TATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATG‑TTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGC > NC_000913/1741496‑1741767
|
tataGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATg > 2:216161/1‑139 (MQ=255)
aaCGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATG‑tttttttagc > 2:631100/1‑135 (MQ=255)
tGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCtt < 1:631100/139‑1 (MQ=255)
cccGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcg < 1:216161/139‑1 (MQ=255)
cggcggATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg < 1:406281/139‑1 (MQ=255)
tatTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct > 2:141812/1‑139 (MQ=255)
gtgtGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTATGCGCAGGTCTCgaga < 2:92668/139‑1 (MQ=255)
gtgtGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCgaga < 1:916955/139‑1 (MQ=255)
aTTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCcatc < 2:305438/139‑1 (MQ=255)
agaagGCTCTTTGGGGGTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTAt > 2:573397/1‑139 (MQ=255)
ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACgcgc < 1:255602/85‑1 (MQ=255)
ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACgcgc > 2:255602/1‑85 (MQ=255)
ctctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCgg > 1:534029/1‑139 (MQ=255)
ctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCa < 2:969561/98‑1 (MQ=255)
ctTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCa > 1:969561/1‑98 (MQ=255)
aGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg < 1:704321/72‑1 (MQ=255)
aGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTAcgcg > 2:704321/1‑72 (MQ=255)
ggCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct < 2:5808/78‑1 (MQ=255)
ggCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGtct > 1:5808/1‑78 (MQ=255)
gCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCa < 1:130636/139‑1 (MQ=255)
tatGGATGGCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCagag > 1:963387/1‑139 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCca < 2:462104/78‑1 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCca > 1:462104/1‑78 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa > 1:247592/1‑139 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa > 1:1006787/1‑139 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa > 2:50664/1‑139 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa < 2:467478/139‑1 (MQ=255)
ggCTGGTGGGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTaaaaaa > 1:467478/1‑139 (MQ=255)
gtggGAATGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGc > 2:220301/1‑139 (MQ=255)
aTGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGAt > 1:662663/1‑139 (MQ=255)
aTGTGACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGAt < 2:963387/139‑1 (MQ=255)
tgtgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCag > 2:158215/1‑118 (MQ=255)
tgtgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCag < 1:158215/118‑1 (MQ=255)
tgACCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATGATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGt < 1:929438/139‑1 (MQ=255)
aCCGACTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCg > 1:687099/1‑139 (MQ=255)
cTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCaaa < 2:247592/139‑1 (MQ=255)
cTGGATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGATCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCaaa < 2:534029/139‑1 (MQ=255)
gATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTg > 1:912198/1‑103 (MQ=255)
gATATGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTg < 2:912198/103‑1 (MQ=255)
atatGTTTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGc < 2:761935/139‑1 (MQ=255)
|
TATAGCCATTAACGGTTCTGCCCCGGCGGATATTCGTGTGAAAAACCCCGATTTTTTTAAACGCGTTCTGCAAGAAGGCTCTTTGGGGTTAGGCGAAAGTTATATGGATGGCTGGTGGGAATGTGACCGACTGGATATG‑TTTTTTAGCAAAGTCTTACGCGCAGGTCTCGAGAACCAACTCCCCCATCATTTCAAAGACACGCTGCGTATTGCCGGCGCTCGTCTCTTCAATCTGCAGAGTAAAAAACGTGCCTGGATAGTCGGCAAAGAGC > NC_000913/1741496‑1741767
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
