Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I2 R1
|
764 |
42.0 |
2018750 |
95.5% |
1927906 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,761,149 |
G→A |
H215Y (CAT→TAT) |
sufD ← |
component of SufBCD Fe‑S cluster assembly scaffold |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,761,149 | 0 | G | A | 100.0%
| 84.8
/ NA
| 27 | H215Y (CAT→TAT) | sufD | component of SufBCD Fe‑S cluster assembly scaffold |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/14); total (13/14) |
CGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCG > NC_000913/1761015‑1761278
|
cGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATAtactgc > 2:269591/1‑139 (MQ=255)
gtgtTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCACCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTggg > 1:377127/1‑139 (MQ=255)
gTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGc > 2:26782/1‑139 (MQ=255)
acTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGt < 1:269591/139‑1 (MQ=255)
gcgcCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGa > 1:212857/1‑139 (MQ=255)
tgGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAg > 2:999112/1‑96 (MQ=255)
tgGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAg < 1:999112/96‑1 (MQ=255)
aaTGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc < 1:26782/139‑1 (MQ=255)
cGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGACAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCCGTAAAATGACGAGc < 2:613744/139‑1 (MQ=255)
tGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGcccc > 2:78924/1‑121 (MQ=255)
tGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGcccc < 1:78924/121‑1 (MQ=255)
gCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc < 2:402697/130‑1 (MQ=255)
gCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGAc > 1:402697/1‑130 (MQ=255)
tCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCa > 1:745005/1‑139 (MQ=255)
tCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCa > 2:723488/1‑139 (MQ=255)
agccagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTg > 1:433670/1‑139 (MQ=255)
ccagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGAc < 1:376757/139‑1 (MQ=255)
cagcAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACa < 2:745005/139‑1 (MQ=255)
caacaaATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACaaaa > 2:767707/1‑139 (MQ=255)
gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg < 1:924272/85‑1 (MQ=255)
gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg < 1:908802/85‑1 (MQ=255)
gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg > 2:908802/1‑85 (MQ=255)
gCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTg > 2:924272/1‑85 (MQ=255)
aCTGAGTGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTGGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGAt > 2:529518/1‑120 (MQ=255)
aCTGAGCGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGAt < 1:529518/120‑1 (MQ=255)
cGGGTTTTCAAACGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGcc < 2:377127/139‑1 (MQ=255)
aaaCGCCAGCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGccagatccaga < 2:212857/139‑1 (MQ=255)
gCTTGATATACTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCg < 1:723488/139‑1 (MQ=255)
|
CGTACTGGTGTTGTGTCGTAACACTGCGCCACCCAGCAGGAAACTGTGGCTAAATGCGGTGGCATCCTCAGCCAGCAACAAATCGTTATGAGCAAAGTGGTGACTGAGCGGGTTTTCAAACGCCAGCTTGATATGCTGCAAGTGGGCATTCGCTGCGACGTTGATAGTGAACCGTGCCCCGGTAAAATGACGAGCATCATTCAGGCTGACAAAATGTTCGATCACCGTTGCTTCGGCACCTTCCGCCAGATCCAGATGATGTCG > NC_000913/1761015‑1761278
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
