Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I2 R1	764	42.0	2018750	95.5%	1927906	109.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	35 (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	118.6 / NA	37	35 (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/21); total (16/21)

CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAC  >  NC_000913/1873952‑1874212

ctctTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGt                                                                                                                            <  1:754398/139‑1 (MQ=255)

    tCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGa                                                                                                                        >  1:909954/1‑139 (MQ=255)

       aaCGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaa                                                                                                                     <  2:222055/139‑1 (MQ=255)

                atgaAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACgg                                                                                                            <  2:674366/139‑1 (MQ=255)

                     aTCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaa                                                                                                       <  1:50609/139‑1 (MQ=255)

                      tCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTAGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaa                                                                                                      >  1:654791/1‑139 (MQ=255)

                          cTTGAATGCAGCCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAg                                                                                                  <  2:654791/139‑1 (MQ=255)

                                          cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGCCAAAACCAGTACAACAGCAATGGCa                                                                                  >  1:290430/1‑139 (MQ=255)

                                          cTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCa                                                                                  >  1:360977/1‑139 (MQ=255)

                                            ggATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaa                                                                                <  2:909954/139‑1 (MQ=255)

                                                    ccGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATg                                                                        >  2:121352/1‑139 (MQ=255)

                                                      gTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGAc                                                                      <  2:360977/139‑1 (MQ=255)

                                                       ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACg                                                                     >  1:873452/1‑139 (MQ=255)

                                                       ttATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTACCGCCGCACTGCGTAATTAGCCGGTCCGTACGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACg                                                                     >  1:664532/1‑139 (MQ=255)

                                                                    cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                                        >  1:626655/1‑139 (MQ=255)

                                                                      cgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaag                                                                                                                    >  2:889272/1‑77 (MQ=255)

                                                                      cgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATaag                                                                                                                    <  1:889272/77‑1 (MQ=255)

                                                                        gATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCg                                                    <  2:321361/139‑1 (MQ=255)

                                                                          ttattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                  <  1:794842/139‑1 (MQ=255)

                                                                           tattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                                        <  1:224507/100‑1 (MQ=255)

                                                                           tattTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCa                                                                                        >  2:224507/1‑100 (MQ=255)

                                                                                     ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                        <  1:758849/138‑1 (MQ=255)

                                                                                     ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGa                                        >  2:758849/1‑138 (MQ=255)

                                                                                            aCCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGATAAACATTTGATCGTTCAt                                <  2:664532/139‑1 (MQ=255)

                                                                                             ccAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                  <  1:853030/120‑1 (MQ=255)

                                                                                             ccAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGAc                                                  >  2:853030/1‑120 (MQ=255)

                                                                                                       tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                          >  1:933835/1‑54 (MQ=255)

                                                                                                       tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                                          <  2:933835/54‑1 (MQ=255)

                                                                                                       tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTc                                  >  2:431442/1‑126 (MQ=255)

                                                                                                       tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTc                                  <  1:431442/126‑1 (MQ=255)

                                                                                                                   gcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTg         <  2:588980/139‑1 (MQ=255)

                                                                                                                         cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAcc                                                                                                    >  1:90899/1‑42 (MQ=255)

                                                                                                                         cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAcc                                                                                                    <  2:90899/42‑1 (MQ=255)

                                                                                                                         cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAc                                                                            <  2:189577/66‑1 (MQ=255)

                                                                                                                         cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAAc                                                                            >  1:189577/1‑66 (MQ=255)

                                                                                                                          gTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAc  <  2:290430/139‑1 (MQ=255)

                                                                                                                          gTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAc  <  2:626655/139‑1 (MQ=255)

CTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATGCCCCCGGGAGATAGCTGTTTTCAC  >  NC_000913/1873952‑1874212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A