Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,078	C→T	35 (TAG→TAA)	yoaI ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,078	0	C	T	100.0%	70.8 / NA	23	35 (TAG→TAA)	yoaI	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/12); total (11/12)

CCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG  >  NC_000913/1873946‑1874188

ccTCTGCTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCg                                                                                                          <  2:669181/139‑1 (MQ=255)

         ttCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCg                                                                                                 >  1:411031/1‑139 (MQ=255)

                                                         tCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGAt                                                 >  1:762357/1‑139 (MQ=255)

                                                                          cgcgGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAgtgt                                >  2:669442/1‑139 (MQ=255)

                                                                                       cgcattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACAtt                   <  1:669442/139‑1 (MQ=255)

                                                                                         cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  <  2:370802/74‑1 (MQ=255)

                                                                                         cattgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  >  1:370802/1‑74 (MQ=255)

                                                                                           ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAGCGATGACGTGATAATCAGTGTCTAGACAAACATTTGAt               <  2:40454/139‑1 (MQ=255)

                                                                                           ttgcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               <  2:411031/139‑1 (MQ=255)

                                                                                             gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               >  2:335753/1‑137 (MQ=255)

                                                                                             gcgcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGAt               <  1:335753/137‑1 (MQ=255)

                                                                                               gcaACCAGAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGtt           <  1:565528/139‑1 (MQ=255)

                                                                                                      gAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                               <  2:264108/48‑1 (MQ=255)

                                                                                                      gAACTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGAt                                                                                               >  1:264108/1‑48 (MQ=255)

                                                                                                        aCTGGTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATg  >  2:535346/1‑139 (MQ=255)

                                                                                                           ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACaaaaa                                                                             <  1:515731/61‑2 (MQ=255)

                                                                                                           ggTCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAAc                                                                             >  2:515731/1‑61 (MQ=255)

                                                                                                             tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                       >  2:208212/1‑49 (MQ=255)

                                                                                                             tCAATTTCCGCCGCACTGCGTAATTAGCCGGTCCGTTCGATAAgaagaa                                                                                       <  1:208212/49‑1 (MQ=255)

                                                                                                                      gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  >  1:731898/1‑45 (MQ=255)

                                                                                                                      gccgcACTGCGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGAc                                                                                  <  2:731898/45‑1 (MQ=255)

                                                                                                                               cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaa                                                        >  2:400840/1‑62 (MQ=255)

                                                                                                                               cGTAATTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCaaa                                                        <  1:400840/62‑1 (MQ=255)

CCTCTACTCTTCGAACGATTTGATGAAATCGCCTTCAATGCAGGCATGCTGGATAAATCCGTTATTTTGAAAACCGCGGATTATTTGCGCATTGCGCAACCAGAACTGGTCAATTTCCGCCGCACTGCGTAACTAGCCGGTCCGTTCGATAAGAAGAACGGACAAAACCAGTACAACAGCAATGGCAAAAAACGATGACGTGATAATCAGTGTCTCGACAAACATTTGATCGTTCATAGCATG  >  NC_000913/1873946‑1874188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A